천문동(Asparagus cochinchinensis Merrill) 부위별 추출물의 생리활성 및 기능성 물질 분석 Evaluation of Biological activity and their related compounds from Asparagus cochinchinensis extracts원문보기
This study was performed to investigate the biological activities and constituents of methanol extracts from Asparagus cochinchinensis with different parts (whole root, flesh and root bark). To evaluate the biological activities of their methanol extracts, DPPH radical, nitrite scavenging, hydroxyl ...
This study was performed to investigate the biological activities and constituents of methanol extracts from Asparagus cochinchinensis with different parts (whole root, flesh and root bark). To evaluate the biological activities of their methanol extracts, DPPH radical, nitrite scavenging, hydroxyl radical, and ABTS radical scavenging activities, total flavonoid and polyphenol contents, and inhibitory activity toward hyaluronidase were measured, respectively. Among them, root bark showed the highest level in DPPH radical, nitrite scavenging, hydroxyl radical, and ABTS radical scavenging activities, on the other hand, flesh showed the lowest level. Further total flavonoid and polyphenol contents of root bark extract were highest among methanol extracts. The polyphenol content of root bark extract showed 6.3 to 10 times higher amount compared to that of flesh extract. Among methanol extracts of each parts, inhibitory effects of hyaluronidase were not detected. To isolate and identify functional constituents. methanol extracts of each parts were fractioned into four categories using Sep-pak C18 column. Fraction Ⅱ and Fraction Ⅲ of root bark showed high level in DPPH radical scavenging activities, and root bark Fraction Ⅳ also showed anti-inflammatory activity in hyaluronidase inhibition assay. After TLC and HPLC analysis, root bark Fraction Ⅱ and and flesh and root bark Fraction Ⅳ were chosen to analyze antioxidative compound and anti-inflammatory compound, respectively. Root bark Fraction Ⅱ was fractioned into five sub-fractions using preparative HPLC. Among them, sub-fraction 5 showed the highest level in DPPH radical scavenging activity and caffeic acid was isolated and identified from sub-fraction 5 by using LC-MS. Among anti-inflammatory compounds of Fraction Ⅳ in root bark and flesh, Protodioscin was identified by using HPLC and LC-MS/MS. Based on these results, it is suggested that root bark and flesh of Asparagus cochinchinensis could be a good candidate for functional materials in food and pharmaceutical industries.
This study was performed to investigate the biological activities and constituents of methanol extracts from Asparagus cochinchinensis with different parts (whole root, flesh and root bark). To evaluate the biological activities of their methanol extracts, DPPH radical, nitrite scavenging, hydroxyl radical, and ABTS radical scavenging activities, total flavonoid and polyphenol contents, and inhibitory activity toward hyaluronidase were measured, respectively. Among them, root bark showed the highest level in DPPH radical, nitrite scavenging, hydroxyl radical, and ABTS radical scavenging activities, on the other hand, flesh showed the lowest level. Further total flavonoid and polyphenol contents of root bark extract were highest among methanol extracts. The polyphenol content of root bark extract showed 6.3 to 10 times higher amount compared to that of flesh extract. Among methanol extracts of each parts, inhibitory effects of hyaluronidase were not detected. To isolate and identify functional constituents. methanol extracts of each parts were fractioned into four categories using Sep-pak C18 column. Fraction Ⅱ and Fraction Ⅲ of root bark showed high level in DPPH radical scavenging activities, and root bark Fraction Ⅳ also showed anti-inflammatory activity in hyaluronidase inhibition assay. After TLC and HPLC analysis, root bark Fraction Ⅱ and and flesh and root bark Fraction Ⅳ were chosen to analyze antioxidative compound and anti-inflammatory compound, respectively. Root bark Fraction Ⅱ was fractioned into five sub-fractions using preparative HPLC. Among them, sub-fraction 5 showed the highest level in DPPH radical scavenging activity and caffeic acid was isolated and identified from sub-fraction 5 by using LC-MS. Among anti-inflammatory compounds of Fraction Ⅳ in root bark and flesh, Protodioscin was identified by using HPLC and LC-MS/MS. Based on these results, it is suggested that root bark and flesh of Asparagus cochinchinensis could be a good candidate for functional materials in food and pharmaceutical industries.
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