넙치(Paralychthys olivaceus)의 Streptococcus parauberis 감염증에 대한 박테리오파지의 적용 Bacteriopphage application to Streptococcus parauberis infection in olive flounder (Paralychthys olivaceus)원문보기
이번 연구의 목적은 국내 넙치 양식장에서 빈번하게 발생하는 S. parauberis 감염증에 대한 bacteriophage 치료법을 개발하는 것이다. S. parauberis에 감염된 넙치에서 현 phage의 복강접종은 세균의 증식을 억제하여 누적폐사율을 감소하였다. 그리고, 현 phage를 경구로 투여할 경우 근육을 제외한 장, 비장, 간 그리고 신장에서 ...
이번 연구의 목적은 국내 넙치 양식장에서 빈번하게 발생하는 S. parauberis 감염증에 대한 bacteriophage 치료법을 개발하는 것이다. S. parauberis에 감염된 넙치에서 현 phage의 복강접종은 세균의 증식을 억제하여 누적폐사율을 감소하였다. 그리고, 현 phage를 경구로 투여할 경우 근육을 제외한 장, 비장, 간 그리고 신장에서 투여 후 5시간째에 출현하기 시작하였다. 근육에서 phage의 출현은 10시간 후 였고 모든 장기에서 phage는 24시간까지 지속하다가 48시간 이내에서 소실되었다. 그러나, 경구투여 후 S. parauberis 감염이 유도되었을 때 그 phage는 48시간 까지 간에서 검출되었다. 또한, 현 phage를 농도 별로 투여하였을 경우 농도 의존적으로 간에서 검출되었다. 현 phage를 반복적으로 경구 투여하였을 경우 간에서 그 phage는 지속적으로 검출되었고 그 수 또한 증가하였다. 그리고, 그 방법은 효율적으로 S. parauberis 감염증에 대한 폐사를 감소할 수 있었다. 이상의 결과로 현 phage의 효용성은 적어도 S. parauberis 감염증이 빈번하게 발생하는 시기에 반복적으로 경구투여 시 그 감염증에 대한 피해를 감소할 수 있을 것으로 사료된다.
이번 연구의 목적은 국내 넙치 양식장에서 빈번하게 발생하는 S. parauberis 감염증에 대한 bacteriophage 치료법을 개발하는 것이다. S. parauberis에 감염된 넙치에서 현 phage의 복강접종은 세균의 증식을 억제하여 누적폐사율을 감소하였다. 그리고, 현 phage를 경구로 투여할 경우 근육을 제외한 장, 비장, 간 그리고 신장에서 투여 후 5시간째에 출현하기 시작하였다. 근육에서 phage의 출현은 10시간 후 였고 모든 장기에서 phage는 24시간까지 지속하다가 48시간 이내에서 소실되었다. 그러나, 경구투여 후 S. parauberis 감염이 유도되었을 때 그 phage는 48시간 까지 간에서 검출되었다. 또한, 현 phage를 농도 별로 투여하였을 경우 농도 의존적으로 간에서 검출되었다. 현 phage를 반복적으로 경구 투여하였을 경우 간에서 그 phage는 지속적으로 검출되었고 그 수 또한 증가하였다. 그리고, 그 방법은 효율적으로 S. parauberis 감염증에 대한 폐사를 감소할 수 있었다. 이상의 결과로 현 phage의 효용성은 적어도 S. parauberis 감염증이 빈번하게 발생하는 시기에 반복적으로 경구투여 시 그 감염증에 대한 피해를 감소할 수 있을 것으로 사료된다.
Olive flounder, Paralichthys olivaceus, is one of the most valuable fish on marine aquaculture industry of Korea, because its production in 2014 was 43,413 tonnes, approximately 52% of total marine cultured finfish production. In addition, the Korea is the world’s largest producer and consumer of th...
Olive flounder, Paralichthys olivaceus, is one of the most valuable fish on marine aquaculture industry of Korea, because its production in 2014 was 43,413 tonnes, approximately 52% of total marine cultured finfish production. In addition, the Korea is the world’s largest producer and consumer of the fish. Recently, some studies have showed high mortality due to a variety of infectious diseases in the farmed olive flounder. It has been suggests to hinder the development of the industry in terms of production. These infectious diseases included streptococcosis, edwardsiellosis and scuticociliatosis.
Fish streptococcosis, which was caused by Streptococcus iniae, S. parauberis and Lactococcus garvieae, has been considered as an important bacterial disease resulting in massive economic loss in farmed finfish of worldwide aquaculture industry. There is a little information about pathogenesis of S. parauberis in fish. In addition, the pathogen is little known in transmission routes. Epidemiological studies in Korea have showed S. parauberis as major etiological agent for the disease in olive flounder, especially marketed size fish. In addition, the disease by S. parauberis was frequently occurred in spring. Around the same time, the fish was intraperitoneally immunized with a commercial multivalent vaccine, such as Edwardsiella tarda and S. iniae. This could be a greater risk factor toward S. parauberis infection in the fish. On the other hand, antibiotic therapy has frequently used for control the disease in the epizootic period and vaccination. However, it is ineffective to infection due to antibiotic-resistant S. parauberis strain.
The bacteriophage is a virus with a bacterial host and it has been recommended as an alternative therapy to infections by pathogenic bacteria and/or multi-antibiotic resistant bacteria [5]. In aquaculture, bacteriophage therapy has gained increased attention to control bacterial infections during the past years and has considered as a realistic alternative to antibiotics and other antibacterial chemicals [14, 15]. There are many studies about the potential for phage control of important fish pathogens [16–20], such as Flavobacterium psychrophilum, Lactococcus garviae and Pseudomonas plecoglossicida showing subsequent reduction in fish mortality after a phage-based treatment in aquaculture.
1
In the present study, we investigated phage-based treatment to S. parauberis infection frequently occurred in olive flounder farms of Korea. Therefore, the aim of this study was (1) to examine fates of the present phage within olive flounder according to intraperitoneal and oral administrations, (2) to explore phage’s persistence in the fish during S. parauberis infection and usefulness of phage by repetitive oral administration to S. parauberis infection
Olive flounder, Paralichthys olivaceus, is one of the most valuable fish on marine aquaculture industry of Korea, because its production in 2014 was 43,413 tonnes, approximately 52% of total marine cultured finfish production. In addition, the Korea is the world’s largest producer and consumer of the fish. Recently, some studies have showed high mortality due to a variety of infectious diseases in the farmed olive flounder. It has been suggests to hinder the development of the industry in terms of production. These infectious diseases included streptococcosis, edwardsiellosis and scuticociliatosis.
Fish streptococcosis, which was caused by Streptococcus iniae, S. parauberis and Lactococcus garvieae, has been considered as an important bacterial disease resulting in massive economic loss in farmed finfish of worldwide aquaculture industry. There is a little information about pathogenesis of S. parauberis in fish. In addition, the pathogen is little known in transmission routes. Epidemiological studies in Korea have showed S. parauberis as major etiological agent for the disease in olive flounder, especially marketed size fish. In addition, the disease by S. parauberis was frequently occurred in spring. Around the same time, the fish was intraperitoneally immunized with a commercial multivalent vaccine, such as Edwardsiella tarda and S. iniae. This could be a greater risk factor toward S. parauberis infection in the fish. On the other hand, antibiotic therapy has frequently used for control the disease in the epizootic period and vaccination. However, it is ineffective to infection due to antibiotic-resistant S. parauberis strain.
The bacteriophage is a virus with a bacterial host and it has been recommended as an alternative therapy to infections by pathogenic bacteria and/or multi-antibiotic resistant bacteria [5]. In aquaculture, bacteriophage therapy has gained increased attention to control bacterial infections during the past years and has considered as a realistic alternative to antibiotics and other antibacterial chemicals [14, 15]. There are many studies about the potential for phage control of important fish pathogens [16–20], such as Flavobacterium psychrophilum, Lactococcus garviae and Pseudomonas plecoglossicida showing subsequent reduction in fish mortality after a phage-based treatment in aquaculture.
1
In the present study, we investigated phage-based treatment to S. parauberis infection frequently occurred in olive flounder farms of Korea. Therefore, the aim of this study was (1) to examine fates of the present phage within olive flounder according to intraperitoneal and oral administrations, (2) to explore phage’s persistence in the fish during S. parauberis infection and usefulness of phage by repetitive oral administration to S. parauberis infection
주제어
#Bacteriophage application to Streptococcus parauberis 박테리오파지 연쇄상구균증 적용
학위논문 정보
저자
우동욱
학위수여기관
전북대학교 일반대학원
학위구분
국내석사
학과
수의학 수생생명의학
지도교수
신기욱
발행연도
2016
총페이지
i, 33 p.
키워드
Bacteriophage application to Streptococcus parauberis 박테리오파지 연쇄상구균증 적용
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