현재 증가하는 치킨소비로 인해 L. monocytogenes의Listeriosis 발병에 대한 우려가 높아지고 있다. 농촌경제연구원의 예측 정보에 따르면 2005년부터 돼지, 계란, 닭 순으로 소비가 증가하고 있을 뿐만 아니라 소비자 가격측면에서도 가격이 한 해가 지날수록 감소된다. 이는 향후 더욱 큰 치킨의 소비 증가가 예상된다. 이에 대한 방안으로 본 연구에서는 물리적 처리방법인 UV-C light (600, 1200, 1800, and 2400 mJ/cm2), LAE (100, 200 and 400 mg/kg)와 생물학제제인 Listeria Bacteriophages를 이용하여 닭가슴살의 품질 손상 없이 Listeria monocytogenes의 살균효과를 극대화시키고자 한다. UV-C의 조사량과 LAE의 농도가 증가할수록 L. monocytogenes가 유의적으로 (p < 0.05) 감소하였고 Bacteriophages 처리의 경우 1일 경과 후 L. monocytogenes가 유의적으로 감소하였다(p < 0.05). 이에 따라 UV-C light (600, 1200, 1800, and 2400 mJ/cm2)와 bacteriophages로 병용 처리된 닭가슴살은 각각 1.48, 1.66, 1.74, 2.04 log CFU/g 감소하였다. LAE (100, 200, and 400 mg/kg)과 bacteriophages로 처리된 닭가슴살의 L. monocytogenes는 각각 1.24, 1.45, 1.96 log CFU/g 감소하였다. 두 종류의 병용처리된 닭가슴살을 4℃에서 3일 동안 저장 후 pH, ...
현재 증가하는 치킨소비로 인해 L. monocytogenes의Listeriosis 발병에 대한 우려가 높아지고 있다. 농촌경제연구원의 예측 정보에 따르면 2005년부터 돼지, 계란, 닭 순으로 소비가 증가하고 있을 뿐만 아니라 소비자 가격측면에서도 가격이 한 해가 지날수록 감소된다. 이는 향후 더욱 큰 치킨의 소비 증가가 예상된다. 이에 대한 방안으로 본 연구에서는 물리적 처리방법인 UV-C light (600, 1200, 1800, and 2400 mJ/cm2), LAE (100, 200 and 400 mg/kg)와 생물학제제인 Listeria Bacteriophages를 이용하여 닭가슴살의 품질 손상 없이 Listeria monocytogenes의 살균효과를 극대화시키고자 한다. UV-C의 조사량과 LAE의 농도가 증가할수록 L. monocytogenes가 유의적으로 (p < 0.05) 감소하였고 Bacteriophages 처리의 경우 1일 경과 후 L. monocytogenes가 유의적으로 감소하였다(p < 0.05). 이에 따라 UV-C light (600, 1200, 1800, and 2400 mJ/cm2)와 bacteriophages로 병용 처리된 닭가슴살은 각각 1.48, 1.66, 1.74, 2.04 log CFU/g 감소하였다. LAE (100, 200, and 400 mg/kg)과 bacteriophages로 처리된 닭가슴살의 L. monocytogenes는 각각 1.24, 1.45, 1.96 log CFU/g 감소하였다. 두 종류의 병용처리된 닭가슴살을 4℃에서 3일 동안 저장 후 pH, TBARS, 색차를 측정한 결과, 대조군에 비해 유의적 차이가 없었다(p < 0.05). 그러나 외관을 고려한다면 UV 1,800 mJ/cm2와 bacteriophages,200 ppm LAE와 bacteriophages의 병용처리 조건이 닭가슴살의 품질을 유지하면서 L. monocytogenes를 저감화시키는데 효과적이라고 판단된다. 본 연구결과는 닭고기의 미생물학적 안전성 확보기술 개발에 기여할 것이다.
현재 증가하는 치킨소비로 인해 L. monocytogenes의Listeriosis 발병에 대한 우려가 높아지고 있다. 농촌경제연구원의 예측 정보에 따르면 2005년부터 돼지, 계란, 닭 순으로 소비가 증가하고 있을 뿐만 아니라 소비자 가격측면에서도 가격이 한 해가 지날수록 감소된다. 이는 향후 더욱 큰 치킨의 소비 증가가 예상된다. 이에 대한 방안으로 본 연구에서는 물리적 처리방법인 UV-C light (600, 1200, 1800, and 2400 mJ/cm2), LAE (100, 200 and 400 mg/kg)와 생물학제제인 Listeria Bacteriophages를 이용하여 닭가슴살의 품질 손상 없이 Listeria monocytogenes의 살균효과를 극대화시키고자 한다. UV-C의 조사량과 LAE의 농도가 증가할수록 L. monocytogenes가 유의적으로 (p < 0.05) 감소하였고 Bacteriophages 처리의 경우 1일 경과 후 L. monocytogenes가 유의적으로 감소하였다(p < 0.05). 이에 따라 UV-C light (600, 1200, 1800, and 2400 mJ/cm2)와 bacteriophages로 병용 처리된 닭가슴살은 각각 1.48, 1.66, 1.74, 2.04 log CFU/g 감소하였다. LAE (100, 200, and 400 mg/kg)과 bacteriophages로 처리된 닭가슴살의 L. monocytogenes는 각각 1.24, 1.45, 1.96 log CFU/g 감소하였다. 두 종류의 병용처리된 닭가슴살을 4℃에서 3일 동안 저장 후 pH, TBARS, 색차를 측정한 결과, 대조군에 비해 유의적 차이가 없었다(p < 0.05). 그러나 외관을 고려한다면 UV 1,800 mJ/cm2와 bacteriophages,200 ppm LAE와 bacteriophages의 병용처리 조건이 닭가슴살의 품질을 유지하면서 L. monocytogenes를 저감화시키는데 효과적이라고 판단된다. 본 연구결과는 닭고기의 미생물학적 안전성 확보기술 개발에 기여할 것이다.
The inhibitory effect of a commercial bacteriophage preparation (ListShield) on Listeria monocytogenes, alone or in combination with treatment with lauric arginate ethyl ester (LAE), Ultraviolet-C, was studied using artificially inoculated fresh chicken breast tissue. A mixture of three L. monocytog...
The inhibitory effect of a commercial bacteriophage preparation (ListShield) on Listeria monocytogenes, alone or in combination with treatment with lauric arginate ethyl ester (LAE), Ultraviolet-C, was studied using artificially inoculated fresh chicken breast tissue. A mixture of three L. monocytogenes strains (ATCC 19113, ATCC 19115, and ATCC 13932) was inoculated in fresh chicken breast tissue at approximately 4.5 log CFU/g, followed by treatment with UV-C radiation at 260 nm (four dose 600, 1200, 1800, and 2400 mJ/cm2) and/or bacteriophage preparation Listshield. In case of LAE treatment, LAE (100, 200, and 400 mg/kg) was sprayed and/or sprayed bacteriophage preparation Listshield. Tissues samples were analyzed after 5 min, followed by storage at 4°C for 1, 2, and 3 days. The L. monocytogenes with 600-2400 mJ/cm2 of UV-C maximally reduced by 1.23, 1.47, 1.53, and 1.58 log CFU/g, respectively, compared to the untreated control samples. The counts of these bacteria were significantly difference (p < 0.05) reduced by as stepwise increasing of UV-C dosage. After a single round of treatment with bacteriophages, about 0.56, 0.84, 0.46, and 0.10 log CFU/g reduction was observed after day 0, 1, 2, and 3 days, respectively, compared to the control samples. Significant differences were noted between the treated and control samples (p < 0.05) at 0, 1, and 2 days. After combined treatment with UV-C and bacteriophage, the L. monocytogenes was reduced more significantly by 1.48, 1.66, 1.74, and 2.04 log CFU/g, at 0 day (1-3 h) respectively. In case of LAE treatment, the L. monocytogenes with 100, 200, and 400 mg/kg of LAE were reduced by 0.45, 0.69, and 1.15 log CFU/g at 1day respectively, compared to control samples. After combined treatment with bacteriophages and LAE (100, 200, and 400 mg/kg), the L. monocytogenes count was reduced more significantly by 1.24, 1.45, and 1.96 at 1 day respectively. Following 3 days of treatment, the viable bacterial count of samples of combined treatment with bacteriophages and UV-C irradiation gradually increased to approximately 4.0 log CFU/g regardless of all the UV doses. Also, in the combined LAE and Phages treated samples, reached to approximately 4.5 log CFU/g regardless of all concentrations of LAE. However, no significant difference was observed in the surface color, pH, TBARS content, and visual appearance of the treated and control samples during the 3 days post treatment. Thus, a commercial bacteriophage preparation can be very effective in reducing L. monocytogenes load in chicken fillets, either alone or in combination with UV-C light and LAE treatment.
The inhibitory effect of a commercial bacteriophage preparation (ListShield) on Listeria monocytogenes, alone or in combination with treatment with lauric arginate ethyl ester (LAE), Ultraviolet-C, was studied using artificially inoculated fresh chicken breast tissue. A mixture of three L. monocytogenes strains (ATCC 19113, ATCC 19115, and ATCC 13932) was inoculated in fresh chicken breast tissue at approximately 4.5 log CFU/g, followed by treatment with UV-C radiation at 260 nm (four dose 600, 1200, 1800, and 2400 mJ/cm2) and/or bacteriophage preparation Listshield. In case of LAE treatment, LAE (100, 200, and 400 mg/kg) was sprayed and/or sprayed bacteriophage preparation Listshield. Tissues samples were analyzed after 5 min, followed by storage at 4°C for 1, 2, and 3 days. The L. monocytogenes with 600-2400 mJ/cm2 of UV-C maximally reduced by 1.23, 1.47, 1.53, and 1.58 log CFU/g, respectively, compared to the untreated control samples. The counts of these bacteria were significantly difference (p < 0.05) reduced by as stepwise increasing of UV-C dosage. After a single round of treatment with bacteriophages, about 0.56, 0.84, 0.46, and 0.10 log CFU/g reduction was observed after day 0, 1, 2, and 3 days, respectively, compared to the control samples. Significant differences were noted between the treated and control samples (p < 0.05) at 0, 1, and 2 days. After combined treatment with UV-C and bacteriophage, the L. monocytogenes was reduced more significantly by 1.48, 1.66, 1.74, and 2.04 log CFU/g, at 0 day (1-3 h) respectively. In case of LAE treatment, the L. monocytogenes with 100, 200, and 400 mg/kg of LAE were reduced by 0.45, 0.69, and 1.15 log CFU/g at 1day respectively, compared to control samples. After combined treatment with bacteriophages and LAE (100, 200, and 400 mg/kg), the L. monocytogenes count was reduced more significantly by 1.24, 1.45, and 1.96 at 1 day respectively. Following 3 days of treatment, the viable bacterial count of samples of combined treatment with bacteriophages and UV-C irradiation gradually increased to approximately 4.0 log CFU/g regardless of all the UV doses. Also, in the combined LAE and Phages treated samples, reached to approximately 4.5 log CFU/g regardless of all concentrations of LAE. However, no significant difference was observed in the surface color, pH, TBARS content, and visual appearance of the treated and control samples during the 3 days post treatment. Thus, a commercial bacteriophage preparation can be very effective in reducing L. monocytogenes load in chicken fillets, either alone or in combination with UV-C light and LAE treatment.
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