Objectives: Change in expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. To date, no studies have investigated the roles of various C- type lectin receptors (CLRs) in cholesteatoma. I therefore assessed the levels of expression of CLR ...
Objectives: Change in expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. To date, no studies have investigated the roles of various C- type lectin receptors (CLRs) in cholesteatoma. I therefore assessed the levels of expression of CLR m RNAs in normal skin and in cholesteatoma. Methods: Cholesteatoma specimens were obtained from 38 patients with acquired cholesteatoma from Aug 2012 to Dec 2015. The posterior auricular skin of each patient was used as control. The levels of expression of various CLR mRNAs were assessed quantitatively using real-time RT-PCR and correlated with age, sex, the presence of bacteria, hearing level, frequencies of surgery, degree of ossicle destruction. Results: The expression levels of CD206, DEC-205, MGL, CLEC5A, Dectin-2, BDCA2, Mincle, DCIR, Dectin-1, MICL, CLEC12B mRNAs were significantly higher in cholesteatoma than in control skin samples (p<0.05). The levels of CLEC5A, Dectin-1 mRNAs were significantly higher in cholesteatoma from 2 ossicle destroyed than from 1 ossicle destroyded (p<0.05). The levels of MGL, Mincle, Dectin-1, CLEC12B mRNAs were significantly higher in recurrent than in initial cholesteatoma specimens (p<0.05). The level of CLEC5A mRNAs was significantly higher in severe hearing loss group than in mild to moderate hearing loss group (p0.05). Conclusions: The expression levels of various CLR mRNAs were significantly higher in choleteatoma than in normal skin. These results suggest that these molecules are involved in the pathogenesis of cholesteatoma.
Objectives: Change in expression of pattern recognition receptors may be associated with immune responses in patients with cholesteatoma. To date, no studies have investigated the roles of various C- type lectin receptors (CLRs) in cholesteatoma. I therefore assessed the levels of expression of CLR m RNAs in normal skin and in cholesteatoma. Methods: Cholesteatoma specimens were obtained from 38 patients with acquired cholesteatoma from Aug 2012 to Dec 2015. The posterior auricular skin of each patient was used as control. The levels of expression of various CLR mRNAs were assessed quantitatively using real-time RT-PCR and correlated with age, sex, the presence of bacteria, hearing level, frequencies of surgery, degree of ossicle destruction. Results: The expression levels of CD206, DEC-205, MGL, CLEC5A, Dectin-2, BDCA2, Mincle, DCIR, Dectin-1, MICL, CLEC12B mRNAs were significantly higher in cholesteatoma than in control skin samples (p<0.05). The levels of CLEC5A, Dectin-1 mRNAs were significantly higher in cholesteatoma from 2 ossicle destroyed than from 1 ossicle destroyded (p<0.05). The levels of MGL, Mincle, Dectin-1, CLEC12B mRNAs were significantly higher in recurrent than in initial cholesteatoma specimens (p<0.05). The level of CLEC5A mRNAs was significantly higher in severe hearing loss group than in mild to moderate hearing loss group (p0.05). Conclusions: The expression levels of various CLR mRNAs were significantly higher in choleteatoma than in normal skin. These results suggest that these molecules are involved in the pathogenesis of cholesteatoma.
주제어
#C-type lectin receptor Cholesteatoma Otitis media Bacteria
학위논문 정보
저자
김상훈
학위수여기관
경희대학교 대학원
학위구분
국내박사
학과
의학과 이비인후과학전공
지도교수
여승근
발행연도
2016
총페이지
[i], 34 p.
키워드
C-type lectin receptor Cholesteatoma Otitis media Bacteria
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