Analysis of Phytosphingosine in Plant Resources. Phytosphingosine (PS) is one of the major sphingoid bases of sphingolipid and occurs in yeast, plants and some mammalian tissues. The chemical structure of PS contains a C18 long-chain aliphatic amines and three hydroxyl groups in positions 1,3 an...
Analysis of Phytosphingosine in Plant Resources. Phytosphingosine (PS) is one of the major sphingoid bases of sphingolipid and occurs in yeast, plants and some mammalian tissues. The chemical structure of PS contains a C18 long-chain aliphatic amines and three hydroxyl groups in positions 1,3 and 4. The hydroxyl group at C4 position also was typical in the structure of PS in comparison to other long chain bases. The biological functions of PS includes epidermal differentiation, anti-inflammatory, anti-microbial and anti-acne activities. In pharmaceutical science, PS has been incorporated into formulations to prevent or treat syndromes and disorders related to skin. In recent decades, biomass technology has profoundly been employed to produce alternative material resources, especially in natural plant resource. Therefore, the purpose of this study is to analyze PS in plant resources for cosmetics. Based on the derivatization reaction of a primary amine in PS with orthophthalaldehyde (OPA) in the presence of 2-mercaptoethanol, which inducing the fluorescent product, PS can be determined by the HPLC-FLD method using pre-column derivatization. The fluorescent derivative of PS has the excitation and emission wavelength 355 and 450 nanometer, respectively. The Jasco HPLC-FLD system was employed using the column ThermoScientific Hypersil Gold (150x4.6; 5μ) with the isocratic elution of mobile phase MeOH : water 85:15 (v/v) adding 0.1%TEA and flow rate 1 mL/min. The elution in HPLC system was carried out at room temperature. The derivatization of PS with OPA was optimized with the parameters such as the molar ratio of OPA to PS, temperature and time reaction, and the reaction solvent. Besides, the extraction optimization was studied with chloroform as an extraction solvent and the ratio of chloroform to water 6:3 v/v. The HPLC-FLD method was also validated fully with parameters, namely linear range (2-150 pmol), regression factor (R2=0.9995), LOD (1.26 pmol), LOQ (4.2 pmol), precision (below 12%) and accuracy (above 85%). This method was applied to determine PS in plant biomass samples and common plant samples in order to monitor and select sample as material resource for cosmetics.
Analysis of Phytosphingosine in Plant Resources. Phytosphingosine (PS) is one of the major sphingoid bases of sphingolipid and occurs in yeast, plants and some mammalian tissues. The chemical structure of PS contains a C18 long-chain aliphatic amines and three hydroxyl groups in positions 1,3 and 4. The hydroxyl group at C4 position also was typical in the structure of PS in comparison to other long chain bases. The biological functions of PS includes epidermal differentiation, anti-inflammatory, anti-microbial and anti-acne activities. In pharmaceutical science, PS has been incorporated into formulations to prevent or treat syndromes and disorders related to skin. In recent decades, biomass technology has profoundly been employed to produce alternative material resources, especially in natural plant resource. Therefore, the purpose of this study is to analyze PS in plant resources for cosmetics. Based on the derivatization reaction of a primary amine in PS with orthophthalaldehyde (OPA) in the presence of 2-mercaptoethanol, which inducing the fluorescent product, PS can be determined by the HPLC-FLD method using pre-column derivatization. The fluorescent derivative of PS has the excitation and emission wavelength 355 and 450 nanometer, respectively. The Jasco HPLC-FLD system was employed using the column ThermoScientific Hypersil Gold (150x4.6; 5μ) with the isocratic elution of mobile phase MeOH : water 85:15 (v/v) adding 0.1%TEA and flow rate 1 mL/min. The elution in HPLC system was carried out at room temperature. The derivatization of PS with OPA was optimized with the parameters such as the molar ratio of OPA to PS, temperature and time reaction, and the reaction solvent. Besides, the extraction optimization was studied with chloroform as an extraction solvent and the ratio of chloroform to water 6:3 v/v. The HPLC-FLD method was also validated fully with parameters, namely linear range (2-150 pmol), regression factor (R2=0.9995), LOD (1.26 pmol), LOQ (4.2 pmol), precision (below 12%) and accuracy (above 85%). This method was applied to determine PS in plant biomass samples and common plant samples in order to monitor and select sample as material resource for cosmetics.
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