콩 사포닌은 인간의 건강에 많은 이점이 있는 것으로 알려져 있다, 그러나 그룹 a acetyl 사포닌은 콩에서 쓴맛과 떫은 맛을 유발해 콩의 식품 산업적 이용에 부정적인 영향을 미친다. 따라서 그룹 A acetyl 사포닌에 대한 유전적 연구가 필요할 것으로 보인다. 본 실험에서는 풍산나물콩을 EMS 처리하여 얻은 ...
콩 사포닌은 인간의 건강에 많은 이점이 있는 것으로 알려져 있다, 그러나 그룹 a acetyl 사포닌은 콩에서 쓴맛과 떫은 맛을 유발해 콩의 식품 산업적 이용에 부정적인 영향을 미친다. 따라서 그룹 A acetyl 사포닌에 대한 유전적 연구가 필요할 것으로 보인다. 본 실험에서는 풍산나물콩을 EMS 처리하여 얻은 돌연변이체 892계통을 TLC 분석하여 새로운 사포닌 밴드를 보이는 계통인 PE1905를 선발하였다. PE1905와 풍산나물콩의 하배축을 LC-MS 분석한 결과 돌연변이체인 PE1905에서는 wild type인 풍산나물 콩보다 Af 사포닌이 3배, Ac 사포닌이 1.5배 이상 증가하는 대신 Ab 사포닌과 DDMP 사포닌은 감소하는 것을 알 수 있었다. 또한 PE1905에서만 발견되는 새로운 물질은 지금까지 발견되지 않은 Af 사포닌과 Ab 사포닌의 전구체로 추정된다. 변이체의 유전특성 연구를 위해 wild type인 풍산나물콩과 우람콩을 교배하여 F1종자를 얻었고 이를 다시 파종하여 얻은 F2 종자를 TLC 해본 결과 wild type과 mutant tpye의 비가 3:1로 나타났으며 다시 F2 종자를 파종하여 얻은 F3 계통들을 수확하여 TLC 분석을 해본 결과 Wild tpye 동형접합 개체와, 이형접합 개체, Mutant 동형접합 개체가 1:2:1 로 분리되는 것을 확인 했고 이를 통해 본 돌연변이체에 발현된 유전자는 단일열성 유전자라는 것을 알 수 있었다. 해당 변이체의 농업적 형질을 측정한 결과 도복이 심해졌으며 수량, 100립중, 키, 가지 수, 협 수 등이 감소하는 것을 확인 할 수 있었다. 추가적인 연구를 통해 해당 변이체는 사포닌의 생합성 경로 연구에 도움이 될 것으로 보인다.
콩 사포닌은 인간의 건강에 많은 이점이 있는 것으로 알려져 있다, 그러나 그룹 a acetyl 사포닌은 콩에서 쓴맛과 떫은 맛을 유발해 콩의 식품 산업적 이용에 부정적인 영향을 미친다. 따라서 그룹 A acetyl 사포닌에 대한 유전적 연구가 필요할 것으로 보인다. 본 실험에서는 풍산나물콩을 EMS 처리하여 얻은 돌연변이체 892계통을 TLC 분석하여 새로운 사포닌 밴드를 보이는 계통인 PE1905를 선발하였다. PE1905와 풍산나물콩의 하배축을 LC-MS 분석한 결과 돌연변이체인 PE1905에서는 wild type인 풍산나물 콩보다 Af 사포닌이 3배, Ac 사포닌이 1.5배 이상 증가하는 대신 Ab 사포닌과 DDMP 사포닌은 감소하는 것을 알 수 있었다. 또한 PE1905에서만 발견되는 새로운 물질은 지금까지 발견되지 않은 Af 사포닌과 Ab 사포닌의 전구체로 추정된다. 변이체의 유전특성 연구를 위해 wild type인 풍산나물콩과 우람콩을 교배하여 F1종자를 얻었고 이를 다시 파종하여 얻은 F2 종자를 TLC 해본 결과 wild type과 mutant tpye의 비가 3:1로 나타났으며 다시 F2 종자를 파종하여 얻은 F3 계통들을 수확하여 TLC 분석을 해본 결과 Wild tpye 동형접합 개체와, 이형접합 개체, Mutant 동형접합 개체가 1:2:1 로 분리되는 것을 확인 했고 이를 통해 본 돌연변이체에 발현된 유전자는 단일열성 유전자라는 것을 알 수 있었다. 해당 변이체의 농업적 형질을 측정한 결과 도복이 심해졌으며 수량, 100립중, 키, 가지 수, 협 수 등이 감소하는 것을 확인 할 수 있었다. 추가적인 연구를 통해 해당 변이체는 사포닌의 생합성 경로 연구에 도움이 될 것으로 보인다.
Saponin is one of the secondary metabolites widely distributed in the plants like soybeans (Glycine max Merr.). In soybean, they are termed as soyasaponins. The soyasaponins content in soybean vary from 1.8 to 4.4 % on average, depending on the variety. Soyasaponins are mainly divided into group A s...
Saponin is one of the secondary metabolites widely distributed in the plants like soybeans (Glycine max Merr.). In soybean, they are termed as soyasaponins. The soyasaponins content in soybean vary from 1.8 to 4.4 % on average, depending on the variety. Soyasaponins are mainly divided into group A saponins and DDMP saponins. DDMP saponins have beneficial effects on human health, like inhibiting the obesity and growth of cancer cells and preventing the liver damage. In contrast, the group A saponins are known to cause bitter and astringent taste in the soy products and hence they are considered as unwanted in the soybean industries. We develop a mutant populations through 0.3% EMS treatment of seeds of the soybean cultivar Pungsannamul. A total of 892 M3:4 lines were analyzed for their saponin pattern using Thin Layer Chromatography (TLC). One of the mutants, PE1905 was found to show an increased content of saponin Af (a group A saponin component) and a unknown component, which have not been characterized till date. The objective of the present study was to characterize the mutant PE1905. Saponins from the seeds of the mutant PE1905 were extracted using methanol as per the protocols established in previous study and were analyzed using HPLC and LC-PDA/MS/MS. The HPLC and LC-PDA/MS/MS analysis of the mutant shows that the mutant PE1905 show an increased amount of Af saponin and new unidentified components. New compound 4 showed that glucuronic acid (GlcUA) at C-3 position and acetylated glucose–arabinose (AcetylGlc-Ara) sugar chain at C-22 position of SS-A. From this result, the chemical structure of new component was identified and suggested to be a Ab-δ. The mutant PE1905 was crossed with wild type Pungsannamul and Uram with normal saponin components, to develop a F2 and F2:3 populations. These populations were used for the genetic inheritance test for Ab-δ saponin in PE1905. The results showed that the Ab-δ saponin were found to be controlled by a single recessive gene. Eleven sister lines of PE1905 (M7 generation) were planted at the affiliated experiment and practice field of Kyungpook National University (Gunwi, Korea) to evaluate for the agronomic traits. The mutant PE1905 showed severe lodging compared to the wild type. Besides, other agronomical characteristics such as height, number of branches, and number of pods were found to show severe reductions. In conclusion, in the present study, we characterized a group A saponin mutant to reveal the inheritance, biochemical and agronomic differences from the wild type. Further studies enlightening the corresponding saponin variants are expected unveil the new components of the saponin biosynthetic pathway research. In addition, the mutant PE1905 will be a good genetic source to identify the candidate gene controlling group A saponin content in soybean.
Saponin is one of the secondary metabolites widely distributed in the plants like soybeans (Glycine max Merr.). In soybean, they are termed as soyasaponins. The soyasaponins content in soybean vary from 1.8 to 4.4 % on average, depending on the variety. Soyasaponins are mainly divided into group A saponins and DDMP saponins. DDMP saponins have beneficial effects on human health, like inhibiting the obesity and growth of cancer cells and preventing the liver damage. In contrast, the group A saponins are known to cause bitter and astringent taste in the soy products and hence they are considered as unwanted in the soybean industries. We develop a mutant populations through 0.3% EMS treatment of seeds of the soybean cultivar Pungsannamul. A total of 892 M3:4 lines were analyzed for their saponin pattern using Thin Layer Chromatography (TLC). One of the mutants, PE1905 was found to show an increased content of saponin Af (a group A saponin component) and a unknown component, which have not been characterized till date. The objective of the present study was to characterize the mutant PE1905. Saponins from the seeds of the mutant PE1905 were extracted using methanol as per the protocols established in previous study and were analyzed using HPLC and LC-PDA/MS/MS. The HPLC and LC-PDA/MS/MS analysis of the mutant shows that the mutant PE1905 show an increased amount of Af saponin and new unidentified components. New compound 4 showed that glucuronic acid (GlcUA) at C-3 position and acetylated glucose–arabinose (AcetylGlc-Ara) sugar chain at C-22 position of SS-A. From this result, the chemical structure of new component was identified and suggested to be a Ab-δ. The mutant PE1905 was crossed with wild type Pungsannamul and Uram with normal saponin components, to develop a F2 and F2:3 populations. These populations were used for the genetic inheritance test for Ab-δ saponin in PE1905. The results showed that the Ab-δ saponin were found to be controlled by a single recessive gene. Eleven sister lines of PE1905 (M7 generation) were planted at the affiliated experiment and practice field of Kyungpook National University (Gunwi, Korea) to evaluate for the agronomic traits. The mutant PE1905 showed severe lodging compared to the wild type. Besides, other agronomical characteristics such as height, number of branches, and number of pods were found to show severe reductions. In conclusion, in the present study, we characterized a group A saponin mutant to reveal the inheritance, biochemical and agronomic differences from the wild type. Further studies enlightening the corresponding saponin variants are expected unveil the new components of the saponin biosynthetic pathway research. In addition, the mutant PE1905 will be a good genetic source to identify the candidate gene controlling group A saponin content in soybean.
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