Moringa oleifera Lam is belong to Moringacease, and it is called as moringa or miracle tree due to its useful bio-activities and nutritive composition. However, most of studies have been focused on the aerial parts of tree, especially on the leaf, the study of root is still rudimentary. In this stud...
Moringa oleifera Lam is belong to Moringacease, and it is called as moringa or miracle tree due to its useful bio-activities and nutritive composition. However, most of studies have been focused on the aerial parts of tree, especially on the leaf, the study of root is still rudimentary. In this study, the component and bio-activities of root of miracle tree were investigated and compared to its aerial parts to develop pharmaceutical food using various parts of miracle tree. The results are as a follow.
1. The aerial parts of Moringa oleifera Lam
The hot water and ethanol extract from the aerial part of moringa and their subsequent solvent fractions were prepared. The polyphenol contents of the ethylacetate fractions of hot water and ethanol extract were 195.6 and 166.9 mg/mL, respectively. Measuring of scavenging activities against DPPH anion, ABTS cation and nitrite revealed that the ethylacetate fraction of hot water extract showed 78.7, 87.4 and 76.7 μg/mL of RC50, and the ethylacetate fraction of ethanol extract showed 72.4, 85.2 and 77.0 μg/mL, respectively.
In a while, the anti-diabetes activity assay showed that the hexane fractions of hot water and ethanol extract had strong inhibitions against α-amylase and α-glucosidase. This is a first reports of anti-diabetes activities of aerial parts of moringa. The anti-microbial activity assay based on disc diffusion method showed that the hexane and ethylacetate fractions of ethanol extract have broad spectrum of anti-bacterial activities against gram positive bacteria.
In anti-thrombosis activity assay, the ethylacetate fractions of hot water and ethanol extract had strong inhibitions against thrombin and blood coagulation factors determined by thrombin time and aPTT. Also the above fractions showed strong platelet aggregation inhibitory activities. The slight hemolysis activities (317.8 μg/mL of HC50) were only observed in hexane fraction of ethanol extract.
2. The root of Moringa oleifera Lam
The hot water and ethanol extract from the root of moringa and their subsequent solvent fractions were prepared. The polyphenol contents of the ethylacetate fraction of hot water extract was 103.2 mg/mL. Measuring of scavenging activities against DPPH anion, ABTS cation and nitrite revealed that the ethylacetate fraction of hot water extract showed 59.6, 234.0 and 24.7 μg/mL of RC50, respectively.
In a while, the anti-diabetes activity assay showed that the hexane fraction of ethanol extract had strong inhibitions against α-amylase and α-glucosidase. This is a first reports of anti-diabetes activities of root of moringa. The anti-microbial activity assay based on disc diffusion method showed that the hexane and ethylacetate fractions of hot water and ethanol extract have broad spectrum of anti-bacterial activities against gram positive bacteria. In particular, the ethylacetate fraction of ethanol extract showed strong anti-bacterial activities against E. coli and P. aruginosa.
In anti-thrombosis activity assay, the ethylacetate fraction of hot water extract showed strong inhibitions against thrombin and blood coagulation factors determined by thrombin time and aPTT. In a while, the ethylacetate fraction of ethanol extract showed strong inhibitions against only thrombin. The hexane and ethylacetate fractions of hot water and ethanol extract had strong platelet aggregation inhibitory activities. The IC50 of ethylacetate fraction of ethanol extracts was calculated to 8.9 μg/mL, which are more comparable to aspirin, a commercial anti-platelet agent. No hemolysis activities of root extracts and their solvent fractions were observed.
Above results suggest that the root of moringa has strong anti-oxidant, anti-diabetes, anti-bacterial and anti-thrombosis activities. Therefore the root of moringa has a high potential as noble resource of pharmaceutical food.
Moringa oleifera Lam is belong to Moringacease, and it is called as moringa or miracle tree due to its useful bio-activities and nutritive composition. However, most of studies have been focused on the aerial parts of tree, especially on the leaf, the study of root is still rudimentary. In this study, the component and bio-activities of root of miracle tree were investigated and compared to its aerial parts to develop pharmaceutical food using various parts of miracle tree. The results are as a follow.
1. The aerial parts of Moringa oleifera Lam
The hot water and ethanol extract from the aerial part of moringa and their subsequent solvent fractions were prepared. The polyphenol contents of the ethylacetate fractions of hot water and ethanol extract were 195.6 and 166.9 mg/mL, respectively. Measuring of scavenging activities against DPPH anion, ABTS cation and nitrite revealed that the ethylacetate fraction of hot water extract showed 78.7, 87.4 and 76.7 μg/mL of RC50, and the ethylacetate fraction of ethanol extract showed 72.4, 85.2 and 77.0 μg/mL, respectively.
In a while, the anti-diabetes activity assay showed that the hexane fractions of hot water and ethanol extract had strong inhibitions against α-amylase and α-glucosidase. This is a first reports of anti-diabetes activities of aerial parts of moringa. The anti-microbial activity assay based on disc diffusion method showed that the hexane and ethylacetate fractions of ethanol extract have broad spectrum of anti-bacterial activities against gram positive bacteria.
In anti-thrombosis activity assay, the ethylacetate fractions of hot water and ethanol extract had strong inhibitions against thrombin and blood coagulation factors determined by thrombin time and aPTT. Also the above fractions showed strong platelet aggregation inhibitory activities. The slight hemolysis activities (317.8 μg/mL of HC50) were only observed in hexane fraction of ethanol extract.
2. The root of Moringa oleifera Lam
The hot water and ethanol extract from the root of moringa and their subsequent solvent fractions were prepared. The polyphenol contents of the ethylacetate fraction of hot water extract was 103.2 mg/mL. Measuring of scavenging activities against DPPH anion, ABTS cation and nitrite revealed that the ethylacetate fraction of hot water extract showed 59.6, 234.0 and 24.7 μg/mL of RC50, respectively.
In a while, the anti-diabetes activity assay showed that the hexane fraction of ethanol extract had strong inhibitions against α-amylase and α-glucosidase. This is a first reports of anti-diabetes activities of root of moringa. The anti-microbial activity assay based on disc diffusion method showed that the hexane and ethylacetate fractions of hot water and ethanol extract have broad spectrum of anti-bacterial activities against gram positive bacteria. In particular, the ethylacetate fraction of ethanol extract showed strong anti-bacterial activities against E. coli and P. aruginosa.
In anti-thrombosis activity assay, the ethylacetate fraction of hot water extract showed strong inhibitions against thrombin and blood coagulation factors determined by thrombin time and aPTT. In a while, the ethylacetate fraction of ethanol extract showed strong inhibitions against only thrombin. The hexane and ethylacetate fractions of hot water and ethanol extract had strong platelet aggregation inhibitory activities. The IC50 of ethylacetate fraction of ethanol extracts was calculated to 8.9 μg/mL, which are more comparable to aspirin, a commercial anti-platelet agent. No hemolysis activities of root extracts and their solvent fractions were observed.
Above results suggest that the root of moringa has strong anti-oxidant, anti-diabetes, anti-bacterial and anti-thrombosis activities. Therefore the root of moringa has a high potential as noble resource of pharmaceutical food.
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