The disease burden caused by Mycobacterium tuberculosis (MTB) complex continues to decrease in most countries, however, diseases caused by nontuberculous mycobacteria (NTM) becomes a public health problem. To distinguish tuberculosis to NTM infection, a rapid and accurate identification of mycobacte...
The disease burden caused by Mycobacterium tuberculosis (MTB) complex continues to decrease in most countries, however, diseases caused by nontuberculous mycobacteria (NTM) becomes a public health problem. To distinguish tuberculosis to NTM infection, a rapid and accurate identification of mycobacteria is required. This study aimed to compare the diagnostic performance of three real-time PCR assays Advansure TB/NTM real-time PCR (Advansure, LG Life Science, South Korea), Genedia MTB/NTM detection kit (Genedia, Green Cross Medical Science Corp., South Korea) and PowerChek MTB/NTM Real-time PCR kit (PowerChek, Kogenebiotech, South Korea) for the detection MTB complex and NTM in clinical specimens. The sensitivities, specificities, positive predictive values (PPV) and negative predictive values (NPV) were calculated based on the results of concurrently performed AFB culture. Sensitivities of Advansure automated nucleic acid extraction method and boiling method were 91.4% /98.3% for MTB and 68.2% / 90.9% for NTM detection, respectively. The boiling method exhibited higher sensitivity in NTM detection, when compared to automated nucleic acid extraction method. There was no difference between CFX96™ Real-time PCR detection system (Bio-Rad, Hercules, California, USA) and Applied Biosystems™7500 Real-time PCR instrument system (Thermo Fisher Scientific, Massachusetts, USA) for MTB and NTM detection using the Genedia kit. The diagnostic performance of Advansure, Genedia and PowerChek for MTB complex showed excellent agreement with AFB culture results. On the other hand, there was a difference among the three reagents for diagnostic performance of NTM detection.
The disease burden caused by Mycobacterium tuberculosis (MTB) complex continues to decrease in most countries, however, diseases caused by nontuberculous mycobacteria (NTM) becomes a public health problem. To distinguish tuberculosis to NTM infection, a rapid and accurate identification of mycobacteria is required. This study aimed to compare the diagnostic performance of three real-time PCR assays Advansure TB/NTM real-time PCR (Advansure, LG Life Science, South Korea), Genedia MTB/NTM detection kit (Genedia, Green Cross Medical Science Corp., South Korea) and PowerChek MTB/NTM Real-time PCR kit (PowerChek, Kogenebiotech, South Korea) for the detection MTB complex and NTM in clinical specimens. The sensitivities, specificities, positive predictive values (PPV) and negative predictive values (NPV) were calculated based on the results of concurrently performed AFB culture. Sensitivities of Advansure automated nucleic acid extraction method and boiling method were 91.4% /98.3% for MTB and 68.2% / 90.9% for NTM detection, respectively. The boiling method exhibited higher sensitivity in NTM detection, when compared to automated nucleic acid extraction method. There was no difference between CFX96™ Real-time PCR detection system (Bio-Rad, Hercules, California, USA) and Applied Biosystems™7500 Real-time PCR instrument system (Thermo Fisher Scientific, Massachusetts, USA) for MTB and NTM detection using the Genedia kit. The diagnostic performance of Advansure, Genedia and PowerChek for MTB complex showed excellent agreement with AFB culture results. On the other hand, there was a difference among the three reagents for diagnostic performance of NTM detection.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.