Abstract ▼ AI-Helper

Pre-harvest Sprouting (PHS) during wheat harvest season is a major abiotic stress that impairs wheat quantity and quality not only Republic of Korea but also global worldwide. Although germination is influenced by several environmental conditions, which are external factors, it is thought to be due ...

Pre-harvest Sprouting (PHS) during wheat harvest season is a major abiotic stress that impairs wheat quantity and quality not only Republic of Korea but also global worldwide. Although germination is influenced by several environmental conditions, which are external factors, it is thought to be due to the presence and expression of genes related to seed dormancy and germination, which are intrinsic characteristics. The Mother of FT and TFL1( MFT) gene is known to be a very important gene to seed dormancy and it has been found that its expression rises from the seeds of mature wheat placed in low temperature.
Pre-harvest sprouting (PHS) refers to the premature destruction of the dormant due to exposure to moisture and cold condition during the seed ripening causing great damage to the quality and quantity of wheat. The MFT gene was isolated and sequenced and structural analysis was conducted on twenty-two Korean cultivars that encouraged Korean cultivar 'Keumgang' and pre-harvest sprouting highly resistant 'Woori'.
The pre-harvest sprouting assay was performed using twenty-two Korean wheat cultivar and crosslines advanced to F8 generation through 'Keumgang' and 'Woori'. As a result, it was confirmed that germination resistance appeared in 'cross lines' and 'woori'. Further, the accumulation analysis of MFT gene was performed using quantitative real time-PCR according to pre-harvest spouting progress and hormone treatment in heading stage. Also, the yeast two-hybrid assay was conducted to find interacting genes with MFT gens, and the study was discussed how interrelationships between genes affect PHS and seeding dormancy. These results can be useful to PHS resistant breeding program in Korean wheat.

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