This study was performed to evaluate the antioxidant, cytotoxic and anticancer activities of propolis which were prepared from beehives of different regions of Korea. The beehives were collected from nine regions of Korea, i.e., Gangwon, Gyeonggi, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Gyeongbuk, Gye...
This study was performed to evaluate the antioxidant, cytotoxic and anticancer activities of propolis which were prepared from beehives of different regions of Korea. The beehives were collected from nine regions of Korea, i.e., Gangwon, Gyeonggi, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Gyeongbuk, Gyeongnam and Jeju. The collected samples were extracted with ethanol and the yield was measured. Among of the samples, propolis from Chungnam showed the highest contents of both total flavonoid and phenolic compounds, ranging from 0.9 to 9.7 g Quercetin Equivalents(QE) / 100g propolis and 4.8 to 22.4 g GAE / 100g propolis respectively. In the 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay, all samples showed high DPPH free radical scavenging activity(RSA) with the percentage of the 86 ~ 89%, except Jeju sample which showed the lowest activity. Chungnam propolis, which showed the highest total flavonoid and total phenolic compound contents, was selected for further tests. Cell viability was measured to examine the cytotoxicity of Chungnam propolis on NIH-3T3, a normal murine cell line, and DF-1 cells, a non-senescent chicken cell line, as well as the anticancer activity toward HT-29 cells, a colorectoral human cancer cell line. The anticancer activity of Chungnam propolis on colorectal cancer HT-29 cells was further investigated by studying the expression of apoptosis related genes, ie., Bax and Caspase 3, as well as cell cycle arrested p21 by reverse transcription-quantitative polymerase chain reaction(RT-qPCR). As a result, Chungnam propolis was found to induce apoptosis and cell cycle arrest in colorectal cancer HT-29 cells.
In this study, propolis from Chungnam possessed the highest the highest contents of both total flavonoid and phenolic compound, and showed anti-cancer activity to colorectal cancer cells potentially through apoptosis and cell cycle arrest.
This study was performed to evaluate the antioxidant, cytotoxic and anticancer activities of propolis which were prepared from beehives of different regions of Korea. The beehives were collected from nine regions of Korea, i.e., Gangwon, Gyeonggi, Chungbuk, Chungnam, Jeonbuk, Jeonnam, Gyeongbuk, Gyeongnam and Jeju. The collected samples were extracted with ethanol and the yield was measured. Among of the samples, propolis from Chungnam showed the highest contents of both total flavonoid and phenolic compounds, ranging from 0.9 to 9.7 g Quercetin Equivalents(QE) / 100g propolis and 4.8 to 22.4 g GAE / 100g propolis respectively. In the 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging assay, all samples showed high DPPH free radical scavenging activity(RSA) with the percentage of the 86 ~ 89%, except Jeju sample which showed the lowest activity. Chungnam propolis, which showed the highest total flavonoid and total phenolic compound contents, was selected for further tests. Cell viability was measured to examine the cytotoxicity of Chungnam propolis on NIH-3T3, a normal murine cell line, and DF-1 cells, a non-senescent chicken cell line, as well as the anticancer activity toward HT-29 cells, a colorectoral human cancer cell line. The anticancer activity of Chungnam propolis on colorectal cancer HT-29 cells was further investigated by studying the expression of apoptosis related genes, ie., Bax and Caspase 3, as well as cell cycle arrested p21 by reverse transcription-quantitative polymerase chain reaction(RT-qPCR). As a result, Chungnam propolis was found to induce apoptosis and cell cycle arrest in colorectal cancer HT-29 cells.
In this study, propolis from Chungnam possessed the highest the highest contents of both total flavonoid and phenolic compound, and showed anti-cancer activity to colorectal cancer cells potentially through apoptosis and cell cycle arrest.
주제어
#Propolis Apoptosis Anticancer activity Antioxidant activity
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