Antioxidant and Anti-inflammatory Effect of Potentilla chinensis Seringeand Lonicera japonica Thunberg Mixture(PLM) on LPS-induced nflammation in RAW 264.7 Cells
Objectives :
The purpose of this study was to experiment antioxidant and
anti-inflammatory effect of Potentilla chinens...
Antioxidant and Anti-inflammatory Effect of Potentilla chinensis Seringeand Lonicera japonica Thunberg Mixture(PLM) on LPS-induced nflammation in RAW 264.7 Cells
Objectives :
The purpose of this study was to experiment antioxidant and
anti-inflammatory effect of Potentilla chinensis Seringe and Lonicera japonica
Thunberg Mixture(PLM) on LPS-induced Inflammation in RAW 264.7
macrophages.
Methods :
The antioxidant efficacy of PLM was evaluated that total polyphenol and
flavonoid contents, DPPH and ABTS radical scavenging activity, FRAP
assay. ROS and NO levels in LPS-induced RAW 264.7 cells were also evaluated. The anti-inflammatory effects of PLM were evaluated by inflammatory biomarkers production,inflammatory gene expression, and protein phosphorylation in LPS-induced RAW 264.7 cells.
Results :
It was measured that polyphenols and flavonoids whose antioxidant and
anti-inflammatory effects were confirmed, were included in PLM. The results
of DPPH and ABTS radical scavenging activity. ROS level was decreased at
100, 200 ㎍/㎖ and NO level was decreased at all concentration groups of
PLM compared to the control group. In biomarker production, PGE2, TNF-α
& IL-6 decreased at 100, 200 ㎍/㎖ concentrations and IL-1β was
decreased at 200 ㎍/㎖ concentrations of PLM compared to the control group.
In mRNA expression level, iNOS and IL-6 were decreased at all
concentrations, COX-2 and TNF-α were decreased at 100,200 ㎍/㎖, IL-1
β was decreased at 200 ㎍/㎖ concentrations of PLM compared to the
control group, and HO-1 were increased at 100, 200 ㎍/㎖, NQO1 were
increased at all concentration groups of PLM compared to the control group.
In protein phosphorylation level, iNOS, COX-2 were decreased at 100, 200
㎍/㎖ concentrations of PLM compared to the control group. HO-1, NRF2 were
increased at all concentration groups, NQO1 was increased at 100, 200 ㎍/㎖
concentrations of PLM compared to the control group. ERK, p38 were
decreased at 200 ㎍/㎖ concentrations, JNK was increased at all
concentrations of PLM compared to the control group.
Conclusions :
These results indicate that PLM decrease oxidative and inflammatory
effects through inhibition of the MAPK signaling pathway and adjusting
- 53 -
NRF2/HO-1 pathway. Therefore, these results suggest that PLM has
anti-inflammatory and anti-oxidative activities on LPS-induced RAW
264.7 cells
Antioxidant and Anti-inflammatory Effect of Potentilla chinensis Seringeand Lonicera japonica Thunberg Mixture(PLM) on LPS-induced nflammation in RAW 264.7 Cells
Objectives :
The purpose of this study was to experiment antioxidant and
anti-inflammatory effect of Potentilla chinensis Seringe and Lonicera japonica
Thunberg Mixture(PLM) on LPS-induced Inflammation in RAW 264.7
macrophages.
Methods :
The antioxidant efficacy of PLM was evaluated that total polyphenol and
flavonoid contents, DPPH and ABTS radical scavenging activity, FRAP
assay. ROS and NO levels in LPS-induced RAW 264.7 cells were also evaluated. The anti-inflammatory effects of PLM were evaluated by inflammatory biomarkers production,inflammatory gene expression, and protein phosphorylation in LPS-induced RAW 264.7 cells.
Results :
It was measured that polyphenols and flavonoids whose antioxidant and
anti-inflammatory effects were confirmed, were included in PLM. The results
of DPPH and ABTS radical scavenging activity. ROS level was decreased at
100, 200 ㎍/㎖ and NO level was decreased at all concentration groups of
PLM compared to the control group. In biomarker production, PGE2, TNF-α
& IL-6 decreased at 100, 200 ㎍/㎖ concentrations and IL-1β was
decreased at 200 ㎍/㎖ concentrations of PLM compared to the control group.
In mRNA expression level, iNOS and IL-6 were decreased at all
concentrations, COX-2 and TNF-α were decreased at 100,200 ㎍/㎖, IL-1
β was decreased at 200 ㎍/㎖ concentrations of PLM compared to the
control group, and HO-1 were increased at 100, 200 ㎍/㎖, NQO1 were
increased at all concentration groups of PLM compared to the control group.
In protein phosphorylation level, iNOS, COX-2 were decreased at 100, 200
㎍/㎖ concentrations of PLM compared to the control group. HO-1, NRF2 were
increased at all concentration groups, NQO1 was increased at 100, 200 ㎍/㎖
concentrations of PLM compared to the control group. ERK, p38 were
decreased at 200 ㎍/㎖ concentrations, JNK was increased at all
concentrations of PLM compared to the control group.
Conclusions :
These results indicate that PLM decrease oxidative and inflammatory
effects through inhibition of the MAPK signaling pathway and adjusting
- 53 -
NRF2/HO-1 pathway. Therefore, these results suggest that PLM has
anti-inflammatory and anti-oxidative activities on LPS-induced RAW
264.7 cells
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