초록

AI-Helper

RNase A에 對한 RNA의 加水分解反應性은 spermine에 의하여 抑制되었다. Spermine의 濃度가 增加함에 따라 RNase의 活性度는 점점 減少하다가 plateau에 到達되었다. 同一한 條件에서 spermine의 濃度가 增加함에 따라 RNA의 粘性度는 점점 增加되어 RNase의 活性度에서의 spermine의 依存性과 같이 plateau에 이르게 되었다. RNase A에 對한 RNA의 加水分解反應性에 미치는 spermine의 抑制效果는 denatured(變性) DNA에 의하여 救濟되나 native(本性) DNA에 의하여서는 無關하였다. 이들 實驗結果는 spermine에 의하여 RNA의 分子間 aggregation이 일어나며 그로 因하여 RNA의 RNase A에 對한 加水分解反應性이 抑制될 수 있음을 보여준다.

Abstract ▼ AI-Helper

RNA degradation by riboinuclease A (EC 3.1.27.5) was inhibited by spermine. As the concentration of spermine was increased, the ribonuclease activity was decreased gradually until it reached a plateau. Under the same conditions, the viscosity of the RNA increased, as the spermine concentration was i...

RNA degradation by riboinuclease A (EC 3.1.27.5) was inhibited by spermine. As the concentration of spermine was increased, the ribonuclease activity was decreased gradually until it reached a plateau. Under the same conditions, the viscosity of the RNA increased, as the spermine concentration was increased until it reached a plateau in the same manner as the profile of the spermine-dependent ribonuclease activity. The inhibitory effect of spermine on the susceptibility of RNA to the ribonuclease could be relieved by denatured calf thymus DNA, but not by the native DNA. The data here indicate the possibility that the suppress of the RNA susceptibility for the ribonuclease by spermine is brought about by the spermine-induced intermolecular aggregation of the RNA molecules.