초록

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Abstract ▼ AI-Helper

The acid phosphatase (EC 3.1.3.2.) of human serum has shown to have a maximum activity with 0.1 M acetate buffer at pH 5.0 and had an appreciable activity at a low of $0^{\circ}C$ and also a stable character even with a little high temperature of $50^{\circ}C$ Among several hea...

The acid phosphatase (EC 3.1.3.2.) of human serum has shown to have a maximum activity with 0.1 M acetate buffer at pH 5.0 and had an appreciable activity at a low of $0^{\circ}C$ and also a stable character even with a little high temperature of $50^{\circ}C$ Among several heavy metal ions tested, $Na^+$, $K^+$, $Ca^{2+}$, $Mg^{2+}$, $Hg^{2+}$ and $Cd^{2+}$ did not affected significantly, but $Cu^{2+}$ and $Fe^{2+}$ inhibited enzyme activity seriously. On the other hand, addition of $Fe^{3+}$ to incubation mixture, it stimulated the acid phsophatase more than 2.5 times. EDTA had no effect on the enzyme at all, and rather restored some extent of the inhibitory effect by $Fe^{2+}$ From this result, affinity of $Cu^{2+}$ to enzyme active site was stronger than that of $Fe^{2+}$. In the case of $Fe^{3+}$ stimulation, the enzyme activity was increased more than 3.5 times when $Fe^{3+}$ was preincubated with EDTA for 1 min before addition of enzyme and substrate. And also preincubation of enzyme with $Fe^{3+}$ before addition of EDTA and substrate, it showed a little reduced activity but still high stimulation when it compared with control group.