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Abstract ▼ AI-Helper

Recently, we have reported that the nuclear large subunit pre-rRNA of the green alga Chaetomorpha moniligera has an intron. Now we report that the intron in this pre-rRNA self-splices in vitro and that its structure constructed shows that it is one of the group I B introns. The self-splicing reactio...

Recently, we have reported that the nuclear large subunit pre-rRNA of the green alga Chaetomorpha moniligera has an intron. Now we report that the intron in this pre-rRNA self-splices in vitro and that its structure constructed shows that it is one of the group I B introns. The self-splicing reaction of this intron is inhibited by gentamycin or L-arginine. The intron consists of 653 nucleotides. The primary structure of the intron was derived from the sequence of a region of 25S rDNA that contains the intron. Its 5' terminal base is cytosine and 3' terminal base of the 5' exon that is linked to the intron is uracil. On the other hand, on the basis of tripartite structure that is proposed to form for the selection of 3' splice site, 3' terminal base of the intron has been inferred to be guanine on the position 653. The 5' terminal base of the 3' exon that is linked to this guanine is uracil. Like the other group I introns, the intron in pre-rRNA of Chaetomorpha moniligera contains nine base-paired stems P1 through P9, and it contains also four conserved sequence elements in the following order: 5'-P(GAAUGCGGGAGC)-Q(CCUCCGCAGG)-R(CCCCUAGAGA-CUAGA)-S(AUUCGAGAGUCCU)-3'. Major distinct features of this intron differing from most of the other group I introns are that it has an extra stem P6c which is formed between 3' half of the stem P6a and 3' half of the stem P6, and that the stem P8 has a long extension which contains an open reading frame.