초록

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Abstract ▼ AI-Helper

This experiment was carried out to establish the system of mass propagation through tissue culture using sucker and stem in Ramie. The sterilization for tissue culture of Ramie was the better treatment of 2% NaClO for 20 minute into ultrasonic cleaner than the others, and so rate of contamination wa...

This experiment was carried out to establish the system of mass propagation through tissue culture using sucker and stem in Ramie. The sterilization for tissue culture of Ramie was the better treatment of 2% NaClO for 20 minute into ultrasonic cleaner than the others, and so rate of contamination was 3.3%, and it was able to produce 96% healthy plant. The effect of growth regulator was superior to mixed treatment of 0.02mg/$\ell$ NAA, 1.5mg/$\ell$ BA, 0.lmgmg/$\ell$ GA$_3$, which it was not formed callus and but produced 96% healthy plant. The effect of propagation was higher in culturing of the stem node than the sucker in cultural part, local variety than improved ones. The effect of acclimatization was superior to pretreatment of 30 minute after soaking in 100ppm NAA, transplanting on bed soil which mixed to ratio of vermiculite : soil : sand =1 : 2 : 1, the transplanted plants were grown all normal.

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