능성어, Epinephelus septemfasciatus의 번식생물학적 연구 I. HCG 처리에 의한 배란유도 Reproductive Bioligy of the Sevenband Grouper, Epinephelus septemfasciatus I. The Effect of HCG on Ovulation Induction원문보기
이 실험은 능성어의 친어관리와 종묘생산을 위한 연구의 일환으로 HCG 처리에 의한 난의 성숙과 배란유도를 알아보고자 행하였다. 제주대학교 해양연구소와 한라수산에서 1996년 6월에서 8월에 걸쳐 300-400 g, 1,500-2,000 g, 2,500-4,000 g 되는 능성어를 대상으로 하여 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리하였다. 이들의 생식소 발달은 일반적인 paraffin 절편법에 의하여 haematoxylin과 eosin으로 비교염색하여 조사하였다. 체중 300-400 g, 1,500-2,000 g, 2,500-3,200 g 되는 능성어에 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리한 결과 생식소는 난원세포군, 20~$30\mu$m 내외의 어린 난모세포를 가지고 있었다. 그러나 체중 3,400-4,000 g 되는 능성어에 HCG 1,000 IU/kg 농도로 2회 처리한 결과 난경 ~$800\mu$m되는 건강한 난과 퇴행란이 배란되었다. 체중 4,000 g 개체에서 배란된 난수는 4,252개 였으며, 이 중에서 건강한 난은 20.8%, 퇴행란은 79.2%였다. 그리고 생식소내에는 배란 흔적과 함께 난경 ~$50\mu$m전후의 주변인기 난모세포, 20~$30\mu$m의 어린 난모세포들이 차지하고 있다.
이 실험은 능성어의 친어관리와 종묘생산을 위한 연구의 일환으로 HCG 처리에 의한 난의 성숙과 배란유도를 알아보고자 행하였다. 제주대학교 해양연구소와 한라수산에서 1996년 6월에서 8월에 걸쳐 300-400 g, 1,500-2,000 g, 2,500-4,000 g 되는 능성어를 대상으로 하여 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리하였다. 이들의 생식소 발달은 일반적인 paraffin 절편법에 의하여 haematoxylin과 eosin으로 비교염색하여 조사하였다. 체중 300-400 g, 1,500-2,000 g, 2,500-3,200 g 되는 능성어에 HCG를 500-1,000 IU/kg 체중농도로 2-3회 처리한 결과 생식소는 난원세포군, 20~$30\mu$m 내외의 어린 난모세포를 가지고 있었다. 그러나 체중 3,400-4,000 g 되는 능성어에 HCG 1,000 IU/kg 농도로 2회 처리한 결과 난경 ~$800\mu$m되는 건강한 난과 퇴행란이 배란되었다. 체중 4,000 g 개체에서 배란된 난수는 4,252개 였으며, 이 중에서 건강한 난은 20.8%, 퇴행란은 79.2%였다. 그리고 생식소내에는 배란 흔적과 함께 난경 ~$50\mu$m전후의 주변인기 난모세포, 20~$30\mu$m의 어린 난모세포들이 차지하고 있다.
The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~40...
The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~400g, 1,500~2,000g and 2,500~4,000g, were collected from June to August, 1996. They were treated two or three times with HCG (500~1,000 IU/kg). Gonadal development of the sevenband groupers were examined using the paraffin section method and then stained with haematoxylin and eosin. When smaller groups of sevenband grouper having 300~400g, 1,500~2,00g and 2,500~3,200g in weight were treated two or three time with HCG 9500~1,000 IU/g), their gonads contained oogonia, oocytes with the size of 20~30${\mu}{\textrm}{m}$, and stroma tissues. However, in case of two HCG treatments with a dosage of 1,000 IU/kg on sevenband groupers of 3,400~4,000g, both healthy eggs of $800\mu$m in diameter and regressing eggs were ovulated. A sevenband grouper of 4,000g obvulated 4,252 eggs in total. Of these, healthy and regressing eggs turned out to be 20.8% and 79.2%, respectively. In addition, previtellogenic oocytes of around $50\mu$m, oocytes of 20~$30\mu$m, and ovulation trace were also observed in the gonad.
The experiment was conducted to understand the effect of HCG upon ovulation induction and egg maturation in the sevenband grouper, Epinephelus septemfasciatus, as a part of the research for its resources management and seed production. Three different size groups of sevenband groupers showing 300~400g, 1,500~2,000g and 2,500~4,000g, were collected from June to August, 1996. They were treated two or three times with HCG (500~1,000 IU/kg). Gonadal development of the sevenband groupers were examined using the paraffin section method and then stained with haematoxylin and eosin. When smaller groups of sevenband grouper having 300~400g, 1,500~2,00g and 2,500~3,200g in weight were treated two or three time with HCG 9500~1,000 IU/g), their gonads contained oogonia, oocytes with the size of 20~30${\mu}{\textrm}{m}$, and stroma tissues. However, in case of two HCG treatments with a dosage of 1,000 IU/kg on sevenband groupers of 3,400~4,000g, both healthy eggs of $800\mu$m in diameter and regressing eggs were ovulated. A sevenband grouper of 4,000g obvulated 4,252 eggs in total. Of these, healthy and regressing eggs turned out to be 20.8% and 79.2%, respectively. In addition, previtellogenic oocytes of around $50\mu$m, oocytes of 20~$30\mu$m, and ovulation trace were also observed in the gonad.
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