초록

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해삼의 인공종묘생산 기술개발을 위한 생물학적 기초자료로 이용하고자 산란유발, 유생발달 과정, 유생 사육시 먹이와수용밀도에 관한 시험을 실시한 결과는 다음과 같다. 산란유발은 수온자극에서 6.0~17.5%의 반응이 있었으며, 간출자극에서는 1.4~4.0%로 낮은 반응률을 보였다. 산란량은 50~500만개였고, 수정률은 84.0~96.0%였으며, 부화율은 71.4~84.6%로 나타났다. 해삼의 수정난은 $154{\mu}m$이며, 난발생은 수온 $23^{circ)C$에서 수정후 2시간 10분만에 4세포기, 3시간 40분이 지나면 16세포기로 되며, 14시간 30분 후에는 포배기로 되면서 부화하였다. 그 이후 3일째에는 auricularia 유생으로 되었고, 11일째에는 doliolaria 유생, 15일째에 pentactula 유생으로 변태하였다. 유생의 먹이로는 Chaetoceros calcitrans와 Pav-lova lutheri가 적당한 것으로 나타났으나, 이 중 Chaetoceros calcitrans는 변태가 빠르고 생존율도 높아 해삼 유생의 먹이로 가장 좋았으며, 유생 사육을 위한 최적 밀도는 2개체/ml 이하를 유지하는 것이 좋은 것으로 나타났다.

Abstract ▼ AI-Helper

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995....

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995. Spawning induction rate by the water temperature was 6.0~17.5% and air dry was responsed 1.4~4.0%. Number of eggs spawned of Stichopus japonicus were $50~500\times10^4$ individuals, the fertilization and hatching rate were ranged 84.0~96.0%, 71.4~84.6% respectively. The fertilized egg of Stichopus japonicus appeared mean diameter of $154{\mu}m$. At a constant water temperature of $23^{circ)C$, it become 4 tell stage from fertilization after 2 hours 10 minutes, hatching larvae after 14 hours half, auricularia larvae after 3 days, doliolaria larvare after 11 days and pentactula larvae after 15 days ready for settlement. The suitable food in the larvae reared for 17 days after fertilization were shown the best growth and survival in the larvae food of Chaetoceros calcitrans. Optimum density for larvae rearing were maintained of the larval density lower than 2 individuals/ml.

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