한국내 분포하는 다년생 초본인 삽주 집단의 유전적 다양도와 집단구조를 조사하기 위해 전분 전기영동으로 분석하였다. 15 대립유전자좌위당 9개 좌위에서 다형현상(60.0%)을 보였으며, 유전적 다양도는 종수준에서 0.144로 높은 반면 집단수준이 이보다 약간 낮았다. 삽주의 유전적 다양도는 대부분 집단내에 존재하였고 유사한 생활양식을 가진 다른 식물종에 비해 높았다. 그 이유로는 유성생식, 다년생, 다산 등에 기인한다. 집단간 분화는 약 13%였고 지리적 거리와 유전적 거리의 상관은 높았다(r=0.65). 그럼에도 불구하고 일부 격리된 집단은 유효집단크기를 가지지 못하여 이형접합체의 결여가 유의성을 보여 다양도가 높은 집단의 보존이 요망된다.
한국내 분포하는 다년생 초본인 삽주 집단의 유전적 다양도와 집단구조를 조사하기 위해 전분 전기영동으로 분석하였다. 15 대립유전자좌위당 9개 좌위에서 다형현상(60.0%)을 보였으며, 유전적 다양도는 종수준에서 0.144로 높은 반면 집단수준이 이보다 약간 낮았다. 삽주의 유전적 다양도는 대부분 집단내에 존재하였고 유사한 생활양식을 가진 다른 식물종에 비해 높았다. 그 이유로는 유성생식, 다년생, 다산 등에 기인한다. 집단간 분화는 약 13%였고 지리적 거리와 유전적 거리의 상관은 높았다(r=0.65). 그럼에도 불구하고 일부 격리된 집단은 유효집단크기를 가지지 못하여 이형접합체의 결여가 유의성을 보여 다양도가 높은 집단의 보존이 요망된다.
The study of genetic diversity was carried out in Atractylodes japonica $K_{OIDZ}$. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and geneti...
The study of genetic diversity was carried out in Atractylodes japonica $K_{OIDZ}$. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of eight Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level $(H_{es}=0.144)$, whereas, that of the population level was relatively low $(H_{ep}=0.128)$. Nearly 87% of the total genetic diversity in A. japonica was apportioned within populations. The sexual reproduction, high fecundity, and perennials are proposed as possible factors contributing to high genetic diversity. The indirect estimated of gene flow based on Gst was 1.69.
The study of genetic diversity was carried out in Atractylodes japonica $K_{OIDZ}$. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of eight Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level $(H_{es}=0.144)$, whereas, that of the population level was relatively low $(H_{ep}=0.128)$. Nearly 87% of the total genetic diversity in A. japonica was apportioned within populations. The sexual reproduction, high fecundity, and perennials are proposed as possible factors contributing to high genetic diversity. The indirect estimated of gene flow based on Gst was 1.69.
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가설 설정
In this paper we investigated allozyme variation in Japanese and Korean populations of Atractylodes japonica. 4 japonica, a widespread herb occurring throughout cooler, more temperate regions of the Northern Hemisphere is most abundant in the boreal and Artic areas.
대상 데이터
The leaf samples were collected from eight natural populations of 4 japonica (Fig. 1). More than 36 individuals were analyzed for each population.
이론/모형
, 1992). Observed heterozygosity (HO) was compared with the Hardy-Weinberg expected value using Wrights fixation index (F) (Wright, 1965). These indices were tested for deviation from zero by x2-statistics following Li & Horvitz (1953).
The absolute population differentiation (DM)was calculated using Neis (1973) statistics. The correlation between geographical and genetic distances was evaluated using the modified Mantels test (Smouse et al., 1986).
, 1989) was used to conduct a cluster analysis on Neis genetic distances via the unweighted pairwise groups method arithmetic average (UPGMA). The genetic structure within and among populations was also evaluated using Wrights (1965) F—statistics, FIT, FIS and FST. The FIT and FIS coefficients measure excesses of homozygotes relative to the panmictic expectations in the entire samples and within populations, respectively.
The procedures for the homogenization of tissues, starch gel electrophoresis, and enzyme assays, followed the methods of Soltis et al. (1983). Young leaves were homogenized in Tris-HCl grinding buffer with PVP (pH 8.
성능/효과
410) (Table 2). Analysis of fixation indices, calculated for all polymorphic loci in each populatiion, showed also a slight deficiency of heterozygotes relative to Hardy- Weinberg expectations (Table 3). For example, all fixation indices were positive (46/46), of which 30 indices (65.
129, indicating that about 13% of the total allozyme variation was among populations. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a slight deficiency of heterozygotes relative to Hardy-Weinberg expectations (Table 3).
japonica populations. Nine of the 15 loci examined (60.0%) showed polymorphisms in at least one population, while the remaining six loci (Idh-2, Mdh-l, Per-3, Pgi~2, Sod~l, and Sod-2) were monomorphic in all populations. The percentage of polymorphic loci within populations ranged from 26.
028). On a per locus basis, the proportion of total genetic variation due to differences among populations (GST) ranged from 0.037 for Idh-l to 0.380 for Per-2, with a mean of 0.129, indicating that about 13% of the total allozyme variation was among populations. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a slight deficiency of heterozygotes relative to Hardy-Weinberg expectations (Table 3).
참고문헌 (21)
Edwards, A.L. and R.R. Sharitz. 2000. Population genetics of two rare perennials in isolated wetlands: Sagittaria isoetiformis and S. tens (Alismataceae). Am. J. Bot. 87 : 1147-1158
Hamhck, J.L. and M.J.W. Godt. 1989. Allozyme diversity in plant species, In: Plant Population Genetics, Breeding and Genetic Resources. Brown A.D.H., Clegg M.T., Kahler A.L. andWeir B.S. (eds), Sinauer, Sunderland, MA. pp. 43-63
Hamrick J.L., M.J.W. Godt and S.L. Shennan-Broyles. 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6 : 95-124
Nevo, E., A, Beiles and R. Ben-Shlomo. 1984. The evolutionary significance of genetic diversity : ecological, demographic and life history correlates, In : Evolutionaiy Dynamics of Genetic Diversity. G.S. Many (ed.), Sphnger Verlag, Berlin, pp. 13 21
SAS Institute Inc. 1989. SAS/STAT User's Guide : Ver. 6. SAS Institute. Cary NC
Smouse, P.E., J.C. Long and R.R. Sokal. 1986. Multiple regression and correlation extensions of the Mantel test of matrix correspondence. Syst. Zool. 35 : 627-632
Soltis, D.E., C.H, Haufler, D.C. Danow and G.J. Gastony, 1983. Starch gel elect.rophoresis of ferns '. A compilation of grinding buffers, gel and electrode buffers, and staining schedules. Am. Pem J. 73:9-27
Wahlund, S. 1928. Zusammersetung von Populationen und Korrelation-sercheiunungen von Standpunkt der Verebungslehre aus betrachtet. Hereditas ll: 65-106
Workman, P.L. and J.D. Niswander. 1970. Population studies on southern Indian tribes. II. local genetic differentiation in the Papago. Am. J. Hum. Genet. 22 : 24-49
Wright, S. 1951, The genetical structure of populations. Ann. Eug. 15 : 313-354
Wright, S. 1965. The interpretation of population structure by F- statistics with special regard to systems of mating. Evolution 19 : 395-420
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