[논문]The Effect of Polysaccharide from Angelica Gigas Nakai on Controlling the Differentiation of Human Embryonic Stem Cells

Park, Young-S.; Lee, Jae-E.; Lee, Seo-H.; Lee, Hyeon-Y.

The Effect of Polysaccharide from Angelica Gigas Nakai on Controlling the Differentiation of Human Embryonic Stem Cells 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.10 no.4, 2002년, pp.237 - 242

Park, Young-S. (O. Z. Tech, Hitech Venture Town) , Lee, Jae-E. (O. Z. Tech, Hitech Venture Town) , Lee, Seo-H. (School of Biotechnology and Bioengineering, Kangwon National University) , Lee, Hyeon-Y. (School of Biotechnology and Bioengineering, Kangwon National University)

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It was found that the purified extract from A. gigas Nakai (polysaccharide, M.W., 25 kD) controled differentiating human ES cells. Its optimal supplementation concentration was decided as 0.8 $({\mu}g/ml)$ to efficiently control the differentiation. It also enhanced the cell growth, compared to the control. However, most widely used and commercially available differentiating agent, Leukemia Inhibitory Factor (LIF) negatively affected on the cell growth even though it controls the differentiation of ES cells, down to 40-50 % based on morphological observation and telomerase activity. It was presumed that the extract first affected on cell membrane and resulted in controlling signal system, then amplify gene expression of telomere, which enhanced the telomerase activity up to three times compared to the control. LIF only increased the enzyme activity up to two times. It was confirmed that the extract from A. gigas Nakai could be used for substituting currently used differentiation controlling agent, LIF from animal resources as a cheap plant resource and not affecting the cell growth. It can broaden the application of the plants not only to functional foods and their substitutes but also to fine chemicals and most cutting-edge biopharmaceutical medicine.

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However, most of the work has been focused on screening and identifying biological activities of roots and other parts of 4 gigas, not expanding the possibility of using its extracts as an biological modulator on molecular and cell levels. Therefore, in this work, the possibility of new types of biologically active chemicals from plant resources, A. gigas Nakai will be demonstrated for the use of mammalian culture systems to control the differentiation of human ES cells in vitro.

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The Effect of Polysaccharide from Angelica Gigas Nakai on Controlling the Differentiation of Human Embryonic Stem Cells 원문보기

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The Effect of Polysaccharide from Angelica Gigas Nakai on Controlling the Differentiation of Human Embryonic Stem Cells 원문보기

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