[논문]Kinetic Analysis of the Effect of Cell Density on Hybridoma Cell Growth in Batch Culture

Lee, Eun-Yeol

doi:10.1007/bf02935890

Kinetic Analysis of the Effect of Cell Density on Hybridoma Cell Growth in Batch Culture 원문보기

Biotechnology and bioprocess engineering : Bbe, v.7 no.2, 2002년, pp.117 - 120

Lee, Eun-Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University)

Abstract ▼ AI-Helper

The effect of cell density on cell growth was investigated in a suspension batch culture of hybridoma cells. The specific growth rate was found to increase with increasing initial cell density and then to decrease with further increases in initial cell density. In order to quantitatively describe the dependence of specific growth rate on cell density, a kinetic model is proposed, which satisfactorily represents the experimental data.

주제어

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가설 설정

1cells at low initial cell density (10 × 10⁴ cells/mL) do not grow well in serum-free medium, whereas cells at a high initial cell density (40 × 10⁴ cells/mL) grow well. Second, cells possess detoxification ability in their environment. Table 2 shows the effect of toxic metabolites, such as the ammonium ion and lactate, on specific growth rate and maximum cell density in batch culture.

제안 방법

On fitting the proposed model with experimental data, it was assumed that the stimulatory effect or the inhibitory effect can be lumped into a single cell density term, X, As it was difficult to obtain the value of X_m by experiment, Xm was obtained by linear regression extrapolation using the data collected in the declining region of specific growth rate c_니rve. The fitting between the proposed equation and the experimental data is shown in Fig.
The determination of the cell growth rates of hybridoma cells is a prerequisite for the development of a bioreactor system for the com mercial production of monoclonal antibodies, and cell density effects should be considered to determine the cell growth rate. The objectives of this work were to analyze the effect of cell density on hybridoma cell growth and to propose a cell density inhibition model describing this growth phenomenon to predict growth rates in batch and fed-batch culture.

대상 데이터

The hybridoma KAI 12 secreting IgM type antibody against Chlamidia trachomatis L2 type was used in this study. The cell culture medium used was a low serum medium (DMEM supplemented with 1% (v/v) FBS; 2 g/L kanamycin? 2 g/L sodium carbonate, 150 mg/L ox-aloacetate7 0.

이론/모형

Cell viability was found to be above 94% during exponential growth. Glucose concentration was determined by the DNS method.
Viable cells were distinguished by the trypan blue dye exclusion method. Cell viability was found to be above 94% during exponential growth.

참고문헌 (13)

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