[논문]Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid

Jo, Man-Hyun; Ham, In-ki; Park, Sang-kyu; Seo, Gwan-Seok; Han, Gyu-Heung; Woo, In-Shik

Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid 원문보기

Jo, Man-Hyun (Chungcheongnam-do Agricultural Research & Extension Services) , Ham, In-ki (Chungcheongnam-do Agricultural Research & Extension Services) , Park, Sang-kyu (Chungcheongnam-do Agricultural Research & Extension Services) , Seo, Gwan-Seok (Chungcheongnam-do Agricultural Research & Extension Services) , Han, Gyu-Heung (Chungcheongnam-do Agricultural Research & Extension Services) , Woo, In-Shik (Chungcheongnam-do Agricultural Research & Extension Services)

This study was conducted to develop the clonal propagation technique through in vitro culture using basal stem explants in Phalaenopsis hybrid grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when basal stem explants were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 ml/L coconut water, 1 mg/L NAA, 5 mg/L 2iP and 2.5g/L gel rite. PLBs transferred to Hyponex medium were regenerated to plantlets. Plantlets transferred to plastic pots containing spagnum moss were developed and successfully acclimatized under greenhouse. The flower was bloomingly opened in plants regenerated from basal stem explants. The flower was not different from both mother plant and plant induced through clonal propagation of Phalaenopsis hybrid.

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가설 설정

2B). In this study, we investigated the effects of plant growth regulators and culture media on PLB formation and plant regeneration from basal stem explants. The plant growth regulators, cytokinins and auxins, affect differentiation of adventitious shoots, somatic embryos, adventitious roots, and callus formation in vitro (Fujii et al.

대상 데이터

Phalaenopsis are often also called, monopodial plants. The genus has approximately 50 species. The range of this genus is from India, through Southeast Asia, North to the Philippines, and South to Northern Australia.

성능/효과

It was found that when basal stem explants were cultured on various different levels of concentrations of modified VW medium, the highest number of PLB multiplication was obtained from the VW medium including 1.0 mg/L NAA and 5 mg/L 2iP at 20 µmol•m^2_•s^-1 in PPF and 25±1℃ for 8 weeks. PLBs transferred to VW medium were developed to plantlets.
PLB induction. These results suggest that VW medium containing 1.0 mg/L NAA and 5.0 mg/L 2iP with 2.5 g/L gelrite was the best optimal medium for PLB propagation. In previous studies, high BA concentration(10 mg/L) was used to induce PLBs on emerging leaves of seedlings and mature plant (Tanaka et al.

후속연구

in Phalaenopsis hybrid. Further study should be required to understand various factors associated with the optimization for clonal propagation, a uniform growth and flowering.

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Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid 원문보기

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Effect of Plant Growth Regulators on Clonal Production through Basal Stem Explant Cultures of a Phalaenopsis Hybrid 원문보기

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