[국내논문]CDV 함유 혼합백신과 예방접종 스케줄에 따른 강아지의 면역반응 Serological Response of Puppies to the Selected Canine Vaccines and Vaccination Schedules against Canine Distemper Virus원문보기
본 연구에서는 우리나라 실정에 맞는 개 디스템퍼에 대한 예방접종 프로토콜을 마련하기 위하여, 국내에서 사용 중인 4종류의 상업용 백신과 3 가지의 예방접종 스케줄에 따른 강아지의 면역형성 능력을 비교 평가하였다. 생후 6주령에 예방접종을 실시하기 위하여 동물병원에 내원한 120두의 강아지를 4종류의 백신[C, G, K, V (또는 V3)군]과 예방접종 스케줄(2, V4 군)에 따라 20두씩 임의배치 하여 C, G, K, V(또는 3)군은 3주 간격으로 3회(6, 9, 12주), V2 군은 5회(6, 8, 10, 12, 14주), V4 군은 3회(6, 10, 14주)에 피하로 예방접종을 실시하였다. 초유를 섭취한 7마리 강아지의 모체이행 항체의 소장상태를 확인한 결과 모체이행항체는 생후 6주령에 방어수준 이하로 떨어졌다. 백신에 따른 면역형성능에서 V 백신의 면역형성 능력이 다른 백신보다 우수하였으며 백신 종류에 따라 면역형성 능력에 차이가 인정되어 사용되는 백신의 효능을 주기적으로 평가하여야 할 것으로 판단되었다. 그리고 가장 효과적인 예방접종 스케줄은 6주령에 예방접종을 시작하여 3주 간격으로 3회 접종하는 것으로 판단되었다. 그러나 예방접종을 실시한 모든 군의 대부분의 강아지는 생후 9주령까지 항체가 수준이 방어수준 이하로 나타나 개 디스템퍼의 감염을 예방하기 위하여 이 시기까지는 감영위험성이 높은 곳에 노출되는 것을 피해야 할 것으로 생각되었다.
본 연구에서는 우리나라 실정에 맞는 개 디스템퍼에 대한 예방접종 프로토콜을 마련하기 위하여, 국내에서 사용 중인 4종류의 상업용 백신과 3 가지의 예방접종 스케줄에 따른 강아지의 면역형성 능력을 비교 평가하였다. 생후 6주령에 예방접종을 실시하기 위하여 동물병원에 내원한 120두의 강아지를 4종류의 백신[C, G, K, V (또는 V3)군]과 예방접종 스케줄(2, V4 군)에 따라 20두씩 임의배치 하여 C, G, K, V(또는 3)군은 3주 간격으로 3회(6, 9, 12주), V2 군은 5회(6, 8, 10, 12, 14주), V4 군은 3회(6, 10, 14주)에 피하로 예방접종을 실시하였다. 초유를 섭취한 7마리 강아지의 모체이행 항체의 소장상태를 확인한 결과 모체이행항체는 생후 6주령에 방어수준 이하로 떨어졌다. 백신에 따른 면역형성능에서 V 백신의 면역형성 능력이 다른 백신보다 우수하였으며 백신 종류에 따라 면역형성 능력에 차이가 인정되어 사용되는 백신의 효능을 주기적으로 평가하여야 할 것으로 판단되었다. 그리고 가장 효과적인 예방접종 스케줄은 6주령에 예방접종을 시작하여 3주 간격으로 3회 접종하는 것으로 판단되었다. 그러나 예방접종을 실시한 모든 군의 대부분의 강아지는 생후 9주령까지 항체가 수준이 방어수준 이하로 나타나 개 디스템퍼의 감염을 예방하기 위하여 이 시기까지는 감영위험성이 높은 곳에 노출되는 것을 피해야 할 것으로 생각되었다.
This study was undertaken to compare the serological response of dogs to four commercially available combination vaccines and three different vaccination schedules to canine distemper virus (CDV). A total of 120 healthy puppies (20 puppies per group) at 6 weeks of age were randomly assigned to one o...
This study was undertaken to compare the serological response of dogs to four commercially available combination vaccines and three different vaccination schedules to canine distemper virus (CDV). A total of 120 healthy puppies (20 puppies per group) at 6 weeks of age were randomly assigned to one of four vaccines [C, G, K, and V (or V3) groups] and one of vaccination schedules [V2 and V4 groups]. At six, nine, and 12 weeks of age, puppies in each group were vaccinated with one of four combination vaccines subcutaneously. And puppies in V2 and V4 groups were vaccinated with V vaccine every 2 weeks and 4 weeks, respectively. The serological responses to CDV component of the vaccines were determined by measuring SN titers. The immunogenicity of V vaccine was superior to the other vaccines and optimum vaccination schedule was 3 times vaccination with 3 weeks-interval starting vaccination at 6 weeks of age. Although puppies were vaccinated at 6 weeks of age, the geometric mean CDV titers of puppies in all groups by 9 weeks of age were under the protective level. Therefore, prophylactic measures should include isolation of young dogs from the dog population until vaccination can be expected to provide protection.
This study was undertaken to compare the serological response of dogs to four commercially available combination vaccines and three different vaccination schedules to canine distemper virus (CDV). A total of 120 healthy puppies (20 puppies per group) at 6 weeks of age were randomly assigned to one of four vaccines [C, G, K, and V (or V3) groups] and one of vaccination schedules [V2 and V4 groups]. At six, nine, and 12 weeks of age, puppies in each group were vaccinated with one of four combination vaccines subcutaneously. And puppies in V2 and V4 groups were vaccinated with V vaccine every 2 weeks and 4 weeks, respectively. The serological responses to CDV component of the vaccines were determined by measuring SN titers. The immunogenicity of V vaccine was superior to the other vaccines and optimum vaccination schedule was 3 times vaccination with 3 weeks-interval starting vaccination at 6 weeks of age. Although puppies were vaccinated at 6 weeks of age, the geometric mean CDV titers of puppies in all groups by 9 weeks of age were under the protective level. Therefore, prophylactic measures should include isolation of young dogs from the dog population until vaccination can be expected to provide protection.
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제안 방법
way as before1, 34. This study was undertaken to compare the serological response in puppies to four commercially available combination vaccines and three different vaccination schedules against CDV.
A total of 100 μl of the Vero cell suspension (1X 105 cells/well) was added to each well, and the titration plates were incubated at 37℃ in 5% CO2 for 5 days. The test was read microscopically everyday for five days and check cytopathogenic effect (giant cell formation). A standard virus and a positive control serum were included in each round of test.
for each sample period. And the week that each puppy seroconverted, the overall percentage of puppies in each group that had seroconverted at each sample period, and the mean and standard deviation of the week of seroconversion for each group were calculated.
Cumulative percentages of puppies that seroconverted, determined on the basis of serum neutralizing titers against canine distemper virus. All puppies were vaccinated at six, nine, and 12 weeks of age with C, G, K, and V vaccines.
Maternally derived CDV titers of 1:16 or greater was considered to protect puppies from infection, but titers between 1:10 and 1:16 are not protective and may interfere with vaccination. In this study, the authors used puppies with a wide range of maternal SN antibody titer, including some puppies with titers of 1:64 or greater. There were some puppies in vaccine C, G, and K groups which were not seroconverted by 15 weeks of age.
대상 데이터
A total of 120 healthy puppies at 6 weeks of age were included in this study. These puppies were presented for vaccination by owner in 9 local animal clinics between March, 2002 and October, 2002.
To observe the declining pattern of maternal antibodies of puppies, seven healthy mixed-breed puppies were used. These puppies were bom from a bitch which was vaccinated 2 times with commercial combination vaccine containing CDV at 6 month interval before pregnant, and were reared for 7 weeks with the dam.
데이터처리
vaccination. A repeated-measures analysis of variance (ANOVA.) was used to compare between-group titers or differences in regard to number of puppies that had seroconverted at the time of each vaccination, using Tukey's multiple comparison test. A values of p less than 0.
성능/효과
In addition, puppies in vaccine V group seroconverted earlier than puppies in vaccine group C, G, and K groups regardless of SN titers. Thus, results of this study suggest that puppies vaccinated with vaccine V would be protected earlier than those of puppies vaccinated with other vaccines.
참고문헌 (34)
An SJ, Kwon HM, Kim D. Diagnosis of canine distemper by RT-PCR, nested PCR, and nutralization test. J Vet Clin 2000; 17: 13-20
Appel M, Robson DS. A microneutralization test for canine distemper virus. Am J Vet Res 1973; 34: 1459-1463
Blixenkrone-Moller M, Svansson V, Have P, Oorvell C, Appel M, Pedersen IR, Dietz HH, Henriksen P. Studies on manifestations of canine distemper virus infection in an urban dog population. Vet Microbiol 1993; 37: 163-173
Cherpillod P, Tipold A, Griot-Wenk M, Cardozo C, Schmid I, Fatzer R, Schobesberger M, Zurbriggen R, Bruckner L, Roch F, Vandevelde M, Wittek R, Zurbriggen A. DNA vaccine encoding nucleocapsid and surface proteins of wild type canine distemper virus protects its natural host against distemper. Vaccine 2000; 18: 2927-2936
Coyne MJ. Seroconversion of puppies to canine parvovirus and canine distemper virus: A comparison of two combination vaccine. J Am Animal Hospital Association 2000; 36: 137-142
de Vries P, Uytdehaag GFC and Osterhaus ADME. Canine distemper virus ISCOMs but not MV-ISCOMs, protect dogs against CDV infection. J Gen Virol 1988; 69: 2071-2083
Gerber JD, Brown AL. Effect of development and aging on the response of canine lymphocytes to phytohemagglutinin. Infection and Immunity 1974; 10: 695-699
Gemma T, Watari T, Akiyama K, Miyashita N, Shin YS, Iwatsuki K, Kai C, Mikami T. Epidemiological observation on recent outbreaks of canine distemper in Tokyo. J Vet Med Sci 1996; 58: 547-550
Glardon O, Stockli R. Staupeepidemie in der Schweiz: Epidemiologie und Impfanamnese. Schweizwe Archiv fur Tierrheilkinde 1985; 127: 707-716
Greene CE. Immunoprophylaxis and immunotherapy. In Infectious Diseases of the Dogs and Cat. 2nd ed. Ed Greene CE. Philadelphia: WB Saunders. 1998: 725-729
Greene CE, Appel MJ. Canine distemper. In: Infectious disease of the dog and cat, Philadelphia: WB Saunders. 1998: 9-22
Haig DA. Canine distemper immunization with avianised virus. Onderstepoort J Vet Res 1956; 27: 19-53
Harder TC, Kuczka A, Dubberke M, Pohlenz J, Liess B. Ein Ausbruch von Hundestaupe in einem Tierheim imt vakzinierten Hundepopulation. Kleintierpraxis 1991; 36: 305-314
Jones L, Tenorio E, Gorham J, Yilma T. Protective vaccination of ferrets against canine distemper with recombinant pox virus vaccines expressing the H or F genes of rinderpest virus. Am J Vet Res 1997; 58: 590-593
McCaw DL, Thompson M, Tate D, Bonderer A, Chen YJ. Serum distemper virus and parvovirus antibody titers among dogs brought to a veterinary hospital for revaccination. J Am Vet Med Assoc 1998; 213: 72-75
Mori T, Shin YS, Okita M, Hirayama N, Miyashita N, Gemma T, Kai C, Mikami T. The biological characterization of field isolates of canine distemper virus from Japan. J Gen Virol 1994; 75: 2403-2408
O'Brien SE. Serologic response of puppies to the low-passage modified-live canine parvovirus-2 component in a combination vaccine. J Am Vet Med Assoc 1994; 204: 1207-1209
Olson P, Finnsdottir H, Klingebom B, Hedhammar A. Duration of antibodies elicited by canine distemper virus vaccinations in dogs. Vet Rec 1997; 141: 654-655
Olson P, Klingeborn B, Hedhammar A. Serum antibody response to canine parvovirus, canine adenovirus-I, and canine distemper virus in dogs with known status of immunization: study of dogs in Sweden. Am J Vet Res 1988; 49: 1460-1466
Ott OL. Comments on Ferret-Origin Distemper Vaccines. J Am Vet Med Assoc 1966; 149: 669-671
Peacock GV. Heterotypic Virus Vaccines. J Am Vet Med Assoc 1966; 149: 675-680
Pollock RVH, Carmichael LE. Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination. J Am Vet Med Assoc 1982; 180: 37-42
Rikula U, Nuotio L, Sihvonen L. Canine distemper virus neutralising antibodies in vaccination dogs. Vet Rec 2000; 147: 598-603
Rockborn G. An attenuated strain of canine distemper virus in tissue culture. Nature 1959; 184: 822
Schultz RD. Comments about frequency of vaccination. J Am Vet Med Assoc 1995; 207: 1017-1018
Smith CA. Are we vaccinating too much? J Am Vet Med Assoc 1995; 27: 421-425
Tizard I. Immunity in the fetus and newborn. In: Veterinary Immunology, 5th ed. Philadelphia: WB Saunders. 1996: 247250
Tizard I. Vaccination and vaccines. In: Veterinary Immunology, 5th ed. Philadelphia: WB Saunders. 1996: 265-284
Yoon KB, Kang MI, Park NY, and Han DU. Seroepidemiological survey on canine distemper, canine parvovirus, canine parvovirus, canine coronavirus, canine adenovirus type-2, canine para-influenzavirus of dogs by indirect immunofluorescent test. Korean J Vet Res 1995; 35: 75-85
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