[논문]Cloning and Characterization of the Lactate Dehydrogenase Genes from Lactobacillus sp. RKY2

Lee, Jin-Ha; Choi, Mi-Hwa; Park, Ji-Young; Kang, Hee-Kyoung; Ryu, Hwa-Won; Sunwo, Chang-Sin; Wee, Young-Jung; Park, Ki-Deok; Kim, Do-Won; Kim, Do-Man

doi:10.1007/bf02942351

[국내논문] Cloning and Characterization of the Lactate Dehydrogenase Genes from Lactobacillus sp. RKY2 원문보기

Biotechnology and bioprocess engineering : Bbe, v.9 no.4, 2004년, pp.318 - 322

Lee, Jin-Ha (Engineering Research Institute) , Choi, Mi-Hwa (Department of Material and Biochemical Engineering) , Park, Ji-Young (Department of Material and Biochemical Engineering) , Kang, Hee-Kyoung (Engineering Research Institute) , Ryu, Hwa-Won (School of Biological Sciences and Technology and Research Institute for Catalysis) , Sunwo, Chang-Sin (School of Biological Sciences and Technology and Research Institute for Catalysis) , Wee, Young-Jung (Department of Material and Biochemical Engineering) , Park, Ki-Deok (Korea Basic Science Institite Gwang-Ju Barnch, Chonnam National University) , Kim, Do-Won (Department of Physics, Kangnung National University) , Kim, Do-Man (Engineering Research Institute, School of Biological Sciences and Technology and Research Institute for Catalysis, Biology Research Center for Industrial Accelerator, Dongshin University)

Abstract ▼ AI-Helper

Lactic acid is an environmentally benign organic acid that could be used as a raw material for biodegradable plastics if it can be inexpensively produced by fermentation. Two genes (ldhL and ldhD) encoding the L-(+) and D-(-) lactate dehydrogenases (L-LDH and D-LDH) were cloned from Lactobacillus sp., RKY2, which is a lactic acid hyper-producing bacterium isolated from Kimchi. Open reading frames of ldhL for and ldhD for the L and D-LDH genes were 962 and 998 bp, respectively. Both the L(+)- and D(-)-LDH proteins showed the highest degree of homology with the L- and D-lactate dehydrogenase genes of Lactobacillus plantarum. The conserved residues in the catalytic activity and substrate binding of both LDHs were identified in both enzymes.

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제안 방법

Based on the nine LDH gene sequences (GenBank accession numbers for L-LDH: D12591, U26688, U78635, AY205157, and D90340, GenBank accession numbers for D-LDH: X66723, AF071558, M85224, and D90339) degenerate PCR primers were constructed for the isolation of the IdhL and IdhD gene fragments flanked by the highly conserved regions. The forward and reverse primers were designed for the cloning the IdhL and IdhD gene fragments, and were designated L-ldhcF (5'-TTG CTG CIA ACC CAG TTG A-3') and L-klhcR (5*-CGT GTT CAC CCA TGA TGT A-3'), and D-ldhcF (5'-CGT AAC GTT GGT GTT GAC AA-3') and D-kihcR (5'-AGT GIA GAA AGC AGT GTG TG-3'), respectively. The PCR mixture contained 10 mM Tris/HCl (pH 8.

대상 데이터

plantarum IdhL revealed 92 and 9응% sequence identities, respectively. The sequence has been submitted to GenBank under accession number AY513726. In L-LDH, His-195 is essential for its action as an acid/base catalyst [15, 16].

참고문헌 (20)

FEBS Lett. N. Bernard 290 61 1991 10.1016/0014-5793(91)81226-X Bernard, N., T. Ferain, D. Garmyn, P. Hols, and J. Delcour (1991) Cloning of the D-lactate dehydrogenase gene fromLactobacillus delbrueckii subsp. bulgaricus by complementation inEscherichia coli.FEBS Lett. 290: 61-64.

상세보기
Genet. Eng. News. C. Potera 12 1 1992 Potera, C. (1992) Natural lactic acid produced from whey by Ecochem.Genet. Eng. News. 12: 1-22.
Biotechnol. Bioprocess Eng. C. Y. Kim 2 234 2002 10.1007/BF02932977 Kim, C. Y., J. H. Lee, B. H. Kim, S. K. Yoo, E. S. Seo, K. S. Cho, D. F. Day, and D. Kim (2002) Production of mannitol usingLeuconostoc mesenteroides B-1149.Biotechnol. Bioprocess Eng. 2: 234-236.

상세보기
Biotechnol. Bioprocess Eng. J. H. Lee 8 106 2003 10.1007/BF02940265 Lee, J. H., S. O. Lee, G. K. Lee, E. S. Seo, S. S. Chang, S. K. Yoo, D. W. Kim, D. F. Day, and D. Kim (2003) Transglycosylation reaction and raw starch hydrolysis by novel carbohydrolase fromLipomyces starkeyi.Biotechnol. Bioprocess Eng. 8: 106-111.

상세보기
Kor. J. Biotechnol. Bioeng. M. H. Choi 18 70 2003 Choi, M. H., K. S. Cho, H. K. Kang, J. S. Yun, E. S. Seo, H. W. Ryu, S. H. Chang, S. H. Yoon, and D. Kim (2003) Simple and quantitative analysis method for lactic acid by TLC.Kor. J. Biotechnol. Bioeng. 18: 70-73.
Enzyme Microb. Technol. S. Y. Yun 33 416 2003 10.1016/S0141-0229(03)00139-X Yun, S. Y., Y. J. Wee, and H. W. Ryu (2003) Production of optically pure L(+)-lactic acid from various carbohydrates by batch fermentation ofEnterococcus faecalis RKY1.Enzyme Microb. Technol. 33: 416-423.

상세보기
From Microbiol. M. Bobillo 8 153 1991 Bobillo, M. and V. M. Marshall (1991) Effect of salt and culture aeration on lactic and acetate production byLactobacillus plantarum.From Microbiol. 8: 153-160.
J. Microbiol. Biotechnol. H. S. Kim 10 559 2000 Kim, H. S., D. Kim, H. J. Ryu, and J. F. Robyt (2000) Cloning and sequencing of the α-1→6 dextransucrase genes fromLeuconostoc mesenteroides.J. Microbiol. Biotechnol. 10: 559-563.

원문보기 상세보기
Biotechnol. Lett. H. J. Ryu 22 421 2000 10.1023/A:1005609718173 Ryu, H. J., D. Kim, D. W. Kim, Y. Y. Moon, and J. F. Robty (2000) Cloning of dextransucrase gene (fmcmds) from a constitutive dextransucrase hyper-producingLeuconostoc mesenteroides B-512FMCM developed using VUV.Biotechnol. Lett. 22: 421-425.

상세보기
J. Sambrook 2001 Molecular Cloning: A Laboratory Manual 3rd. ed. Sambrook, J., E. F. Fritisch, and T. Maniatis (2001)Molecular Cloning: A Laboratory Manual. 3rd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
Proc. Natl. Acad. Sci. USA S. N. Cohen 69 2110 1973 10.1073/pnas.69.8.2110 Cohen, S. N., A. C. Y. Chang, and L. Hsu (1973) Non-chrosomal antobiotic resistance in bacteria: Genetic transformation ofEscherichia coli by R-factor DNA.Proc. Natl. Acad. Sci. USA 69: 2110-2114.

상세보기
J. Biol. Chem. H. Taguchi 266 12588 1991 10.1016/S0021-9258(18)98939-8 Taguchi, H. and T. Ohta (1991) Cloning, sequencing,and expression inEscherichia coli of the D-lactate dehydrogenase gene ofLactobacillus plantarum.J. Biol. Chem. 266: 12588-12594.

상세보기
J. Biol. Chem. P. S. F. Mézes 260 1218 1985 10.1016/S0021-9258(20)71231-7 Mézes, P. S. F., R. W. Blacher, and J. O. Lampen (1985) Processing ofBacillus cereus 569/H β-lactamase I inEscherichia coli andBacillus substilis.J. Biol. Chem. 260: 1218-1223.

상세보기
J. Biochem. H. Taguchi 115 930 1994 10.1093/oxfordjournals.jbchem.a124441 Taguchi, H. and T. Ohta (1994) Essential role of arginine 235 in the substrate-binding ofLactobacillus plantarum D-lactate dehydrogenase.J. Biochem. 115: 930-936.

상세보기
J. J. Holbrook 191 1975 Enzymes 3rd ed. Holbrook, J. J., A. Liljas, S. J. Steindal, and M. G. Rossmann (1975) Lactate dehydrogenase. pp. 191-292. In: P. D. Boyer, P. D. (ed.).Enzymes. 3rd ed., Academic Press, NY, USA.
Trends Biochem. Sci. A. R. Clarke 14 101 1989 10.1016/0968-0004(89)90131-X Clarke, A. R., T. Atkinson, and J. J. Holbrook (1989) From analysis to synthesis: new ligand binding sites in the lactate dehydrogenase framework.Trends Biochem. Sci. 14: 101-105.

상세보기
J. Biol. Chem. H. Taguchi 268 18030 1993 10.1016/S0021-9258(17)46807-4 Taguchi, H. and T. Ohta (1993) Histidine 296 is essential for the catalysis inLactobacillus plantarum D-lactate dehydrogenase.J. Biol. Chem. 268: 18030-18034.

상세보기
J. Biol. Chem. J. J. Birktoft 258 472 1983 10.1016/S0021-9258(18)33280-0 Birktoft, J. J. and L. J. Banaszak (1983) The presence of a histidine-aspartic acid pair in the active site of 2-hydroxyacid dehydrogenases. X-ray refinement of cytoplasmic malate dehydrogenase.J. Biol. Chem. 258: 472-482.

상세보기
Biochemistry A. R. Clarke 27 1617 1988 10.1021/bi00405a034 Clarke, A. R., H. M. Wilks, D. A. Barstow, T. Atkinson, W. N. Chia, and J. J. Holbrook (1988) An investigation of the contribution made by the carboxylate group of an active site histidine-aspartate couple to binding and catalysis in lactate dehydrogenase.Biochemistry 27: 1617-1622.

상세보기
Biochem. Biophys. Acta K. W. Hart 914 294 1987 Hart, K. W., A. R. Clarke, D. B. Wigley, A. D. B. Waldman, W. N. Chia, D. A. Barstow, T. Atkinson, J. B. Jones, and J. J. Holbrook (1987) A strong carboxylate-arginine interaction is important in substrate orientation and recognition in lactate dehydrogenase.Biochem. Biophys. Acta, 914: 294-298.

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