This study investigated the effects of an ethanol extract of Allium monanthum MAX. (AME) on ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 100～150 g, were divided into 5 groups; normal group (NOR), AME 200 mg/kg treated group (S1), ethanol (35%, 10 mL/kg) treated group (S2...
This study investigated the effects of an ethanol extract of Allium monanthum MAX. (AME) on ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 100～150 g, were divided into 5 groups; normal group (NOR), AME 200 mg/kg treated group (S1), ethanol (35%, 10 mL/kg) treated group (S2), AME 200 mg/kg and ethanol (35%, 10 mL/kg) treated group (S3) and AME 400 mg/kg and alcohol (35%, 10 mL/kg) treated group (S4). AME was fractionated by the following solvents: n-hexane, chloroform, EtOAC and n-BuOH. Antioxidant index of the n-BuOH fraction was 600 ppm, highest among fractions. The growth rate and feed efficiency ratio were decreased by ethanol, but gradually increased to the corresponding level of the normal group by administering AME. The serum ALT activities that were elevated by ethanol were significantly decreased by AME administration. It was also observed that the hepatic activities of SOD, catalase, xanthine oxidase and GSH-Px that were increased by ethanol were also markedly decreased in the AME treated group with compared to ETB. These results suggest that ethanol extracts of Allium monanthum MAX. may have a protective effect on ethanol-induced hepatotoxicity in rat liver.
This study investigated the effects of an ethanol extract of Allium monanthum MAX. (AME) on ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 100～150 g, were divided into 5 groups; normal group (NOR), AME 200 mg/kg treated group (S1), ethanol (35%, 10 mL/kg) treated group (S2), AME 200 mg/kg and ethanol (35%, 10 mL/kg) treated group (S3) and AME 400 mg/kg and alcohol (35%, 10 mL/kg) treated group (S4). AME was fractionated by the following solvents: n-hexane, chloroform, EtOAC and n-BuOH. Antioxidant index of the n-BuOH fraction was 600 ppm, highest among fractions. The growth rate and feed efficiency ratio were decreased by ethanol, but gradually increased to the corresponding level of the normal group by administering AME. The serum ALT activities that were elevated by ethanol were significantly decreased by AME administration. It was also observed that the hepatic activities of SOD, catalase, xanthine oxidase and GSH-Px that were increased by ethanol were also markedly decreased in the AME treated group with compared to ETB. These results suggest that ethanol extracts of Allium monanthum MAX. may have a protective effect on ethanol-induced hepatotoxicity in rat liver.
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제안 방법
AME was orally administered for 6 weeks by being dissolved in a saline solution to contain 200 mg or 400 mg per kg of body weight based on a preliminary experiment. During the experiment, body weight was measured at one week intervals and feed intake every two days, The feed efficiency ratio (FER) of each experimental group was calculated by dividing total body weight increase by the total feed intake for the period. Each rat was fasted for 16 h before sacrifice and blood samples were collected from the carotid artery.
can prevent the oxidative cellular injury induced by oxygen free radicals produced by alcohol in the liver. For the verification of the inhibitory effects against hepatotoxicity, this study compared the estimation of the activities of free radical generating enzymes: xanthine oxidase (XO), free radical scavenging enzyme, superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-Px), and the levels of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) in liver tissue.
데이터처리
The data were analyzed using SPSS Package and represented as mean±standard error (SE). Statistical significance among groups was evaluated by Tukey's test (p<0.05) after one-way analysis of variance.
이론/모형
Protein concentrations were assayed according to the method of Lowry et al. (44) using bovine serum albumin (Sigma) as the standard. The data were analyzed using SPSS Package and represented as mean±standard error (SE).
The level of peroxide was measured by the colorimetrical titration of malondialdehyde with thiobarbituric acid (41), and the level of glutathione was determined by the method of Tietze (42). The activities of serum ALT and AST were estimated using a kit (Asan Pharm, Korea) based on the method of Reitman-Frankel (43).
fraction of Allium monathum MAX. ethanol extract (AME) by Rancimat's test method. The antioxidant index (Al) of each fraction was 1.
성능/효과
Growth rates were not significantly different among the groups from week 1 to 4. However, some differences were found from week 5. At week 6, the alcohol treated (S2) group (2.79±0.064) showed a significant slowdown of the growth rate than the normal control (NOR) group (3.12±0.087) (p<0.05), and the alcohol and AME (S3) group showed an elevated ratio close to that of the NOR group (p<0.05). These results were in concordance with the reports of Decarli (45) and Halsted (46) that the administration of alcohol reduced the body weight.
05). Based on the fact that the increased serum ALT and AST activities by alcohol were significantly reduced due to the administration of AME in this experiment in comparing with the NOR group, it can be concluded that AME can protect liver cells from alcohol-induced damage.
참고문헌 (77)
Lee TB. 1982. Illustrated Flora of Korea Seoul. Hyangmoonsa, Seoul. p 206
Kang SS, Yoon HS, Chang IM. 1988. Natural Products Sciences. Seoul National University Press, Seoul. p 71
Branen AL. 1957. Toxiciligy and biochemistry of BHA and BHT. Am Oil Chem Soc 52: 59-64
Choi U, Shin OH, Chang YS, Shin JI. 1992. Antioxidant activity of ethanol extract from Rhus javanica Linne on edible oil. Korean J Food Sci Technol 24: 320-325
Lim DK, Choi U, Shin DH, Jeong YS. 1994. Antioxidative effect of propolis extract on palm oil and lard. Korean J Food Sci Technol 26: 622-626
Lim DK, Choi U, Shin DH. 1996. Antioxidative activity of some solvent extract from Caesalpinia sappan L. Korean J Food Sci Technol 28: 77-82
Park JH, Kang KC, Baek SB, Lee YH, Rhee KS. 1991. Separation of antioxidant compounds from edible marine algae. Korean J Food Sci Technol 23: 256-261
Cho SY, You BJ, Chang MH. 1994. Screening for the antioxidants in unused marine resources by the polarographic method. Korean J Food Sci Technol 26: 417-421
Kalimoto G, Yoshida M, Shibahra A. 1985. A role of tocopherol on the heat stability of vegetable oils. Nippon Shokuhin Kogyo Gakkashi 38: 30-308
Ui JJ. 1989. Isolation and identification of constituents from antioxidant andhematopoietic factions of panax ginseng C.A. Mayer. PhD Dissertation. Seoul National University, Korea
Kim SD, Do JH, Oh HI. 1981. Antioxidant activity of panaxginseng browning products. J Korean Agric Chem Soc 24: 161-166
Lim DK, Choi U, Shin DH. 1996. Antioxidative activity of some solvent extract from Caesalpinia sappan L. Korean J Food Sci Technol 28: 77-82
Oh YS, Lee ST, Lee WT. 1981. A taxonomic study of the allium species in Korea. Jour Kor Pl Tax 11: 21-41
Shogakukan. 1985. Encyclopedia of Chinese Drug. Shanghai Science ＆ Technology Press, Tokyo, Japan. p 226
Chang JK. 1996. Wild herbs for health. Nexus Co. Ltd, Seoul. p 210.
Ahn HS, Lee CY, Park SH. 1982. An agronomical enumeration of Korean plant resources. Ilchogak, Seoul. p 294
Kim WJ, Choi HS. 2001. Natural Spices. Hyoil Press, Seoul. p 257-261
Lim WK, Park SK, Rhu JW, Sah DM, Lee MS, Rhim KO. 1997. Resources of botany. Seoil Press, Seoul. p 267- 269
Chung HD. 1974. Studies on the contents of chemical constituents of Allium monanthum MAX. Yongnam National University Thesis 8: 345-351
Shogakukan. 1985. Encyclopedia of Chinese drugs. Shanghai Science ＆ Technology Press, Tokyo. p 26
Baek NI, Ahn EM, Park HY, Chang YJ. 2001. Development of biologically active compounds from edible plant Sources-IV. isolation of galactosyldiglyderide from the Allium monanthum MAX. Kor Jour of Life Sci 11: 93-96
Ahn EM, Baek NI. 2000. Development of biologically active compounds from edible plant Sources-IV. isolation of flavonoids from the Allium monanthum MAX. J Korean Soc Agric Chem Biotechnol 43: 341-316
Simic MG, Krel M. 1980. Autooxidation in food and biological systems. In Natural antioxidants. Plenum Press, NY. p 261-282
Oh YS, Lee ST, Lee WT. 1981. A tanonomic study of the Allium species in Korea. Jour Kor Pl Tox 11: 21-41
Kim WB, Lee KK, Lee DW. 1986. Studies on the establishment of cultural practices of Allium grayi REGEL (1) Effect of seeding date with different seed bulbs on the growth and yield. J Kor Soc Hort Sci 27: 15-21
Lee SM, Sang JH, Oh SM, Park SJ. 1988. Studies on the karyotype analysis of Allium grayi REGEL in Korea. J Kor Soc Hort Sci 29: 75-80
Choi JY, Lee IS, Kim SC. 1992. Effects of wild garlic on serum component of cholesterol fed rats. J Kor Oil Chem Soc 9: 73-79
Shirahashi HN, Murakami M, Watanabe A, Ngatsu J, Skakibara H, Tokuda H, Nishino, Lwashima A. 1993. Isolation and identification of anti-tumor-promoting principles from the fresh-water cyanobacterium Phormidium teune. Chem Pharm Bull 41: 1664-1666
Glueck CJ, Hog E, Allen C, Gartside PS. 1980. Effects of alcohol ingestion on lipid and lipoprotein in normal man. Am J Clin Nutr 33: 2287-2293
Figueroa PB, Klitz AP. 1962. Alterations of alcohol dehydrogenase and other enzyme flowing oral alcohol intoxication. Am J Clin Nutr 11: 235-239
Aykac G, Usual M, Yalcin S, Kocak TN, Sivas A. 1978. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicol 36: 71-78
Hammond B, Kontos A, Hess ML. 1985. Oxygen radicals in the adults respiratory distress syndrome, in myocardial ischemia and reperfusion injury, and cerebral vascular damage. Can J Physiol Pharmacol 63: 173-187
Sung KS, Chun C, Kwon YH, Kim KH, Chang CC. 2000. Effect of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in liver of mice. J Ginseng Res 24: 29-34
Fujji M, Ohmachi T, Sagami I, Watanabe M. 1985. Liver microsomal drug metabolism in ethanol treated hamsters. Biochem Pharmacol 34: 3881-3890
Downry JM, Miura Y, Eddy LJ, Chambers DE, Mellert T, Hearse DJ, Yellon DM. 1987. Xanthine oxidase is not a source of free radicals in the isochemic rabbit heart. J Mol Cell Cardiol 19: 1053-1060
Crapo CH, McCord JM, Fridovich I. 1978. Preparation and assay of superoxide dismutase. In Methods enzymol. Fleischer S, Packer L, eds. Academic press, New York. Vol 53, p 382-422
Aebi H. 1974. Catalase. In Methods of enzymatic analysis. 3rd ed. Bergmeyer HU, Bergmeyer J, Grabi M, eds. Verlag chemie, New York. Vol 2, p 673-684
Flohe L, Wolfgng A, Gunzler WA. 1984. Assay of glutathine peroxidase. In Methods in enzymatic analysis. Packer L, ed. Academic Press, New York. p 14-121
Buege JA, Aust SD. 1978. Microsomal lipid peroxidation In Methods in enzymology. ed. Acdemic Press, New York. Vol 52, p 302-310
Tietze F. 1969. Enzymatic methods for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal Biochem 27: 502-522
Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic determination and glutamic pyruvic transaminase. Am J Clin Pathol 28: 56-63
Lowry CH, Rsenbrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193: 256-275
Liber CS, Decarli LM. 1986. The feeding of ethanol in liquid diet. Alcoholism Clin Exp Res 10: 550-560
Koo BK, Dhung JM, Lee HS. 1998. Biochemical evaluation of nutritional status of protein and lipid in patients with alcoholic liver disease. J Korean Soc Food Sci Nutr 27: 1236-1243
Zimmerman HJ. 1981. Chemical hepatic injury and its detection. In Toxicology of the liver. Plaa GL, Hewitt WR, eds. Raven press, New York. Vol 22, p 1-45
Kim MH, Kim CJ. 1991. The effect of the levels of dietary zinc and alcohol consumption on lipid metabolism in the rats. Kor J Nutr 24: 87-86
Yagi K. 1987. Lipid peroxides and human disease. Chem Phys Lipid 45: 337-349
Storch J, Ferber E. 1988. Detergent-amplified chemiluminescence of lucigenin for determination of superoxide anion production by NADPH oxidase and xanthine oxidase. Anal Biochem 169: 262-270
Oei HH, Stroo WE, Burton KP, Schaffer SW. 1982. A possible role of xanthine oxidase in producing oxidative stress in the heart of chronically ethanol treated rats. Res Commun Chem Pathol Pharmacol 38: 454-461
Sharma GR, Gill KD. 1991. Effects of ethanol on Cd- induced lipid peroxidation and antioxidant enzymes in rat liver. Biochem Pharm 42: 89-96
Sunde RA, Hoekstra WG. 1990. Structure, synthesis and function of glutathione peroxidase. Nutr Rev 38: 269-273
Antonenkov VD, Panchenko LF. 1988. Effect of chronic ethanol treatment under partial catalase inhibition on the activity of enzymes related to peroxide metabolism in rat liver and heart. Int J Biochem 20: 823-828
Kino M. 1986. Chronic effects of ethanol under partial inhibition of catalase activity in the rat heart: Light and electron microscopic observation. J Mol Cell Cardiol 8: 22A-32A
Speisky H, Kera Y, Penttila KE, Israel Y, Lindros KO. 1988. Depletion of hepatic glutathine by ethanol occurs independently of ethanol metabolism. Alchol Clin Exp Res 12: 224-28
Yunice AA, Hsu JM. 1986. Alcohol. In Nutritional aspects of aging. Chen LH, ed. CRC Press, Washington, DC. Vol 2, p 19-27
Harata J, Ishiguro I. 1983. Effect of prolonged alcohol administration on activities of various enzymes scavenging activated oxygen radicals and lipid peroxide level in the rat livers. Biochem Pharma 32: 1975-1988
Cheigh HS. 1994. Lipid peroxidation and its nutritional significance. J Korean Soc Food Nutr 23: 867-871
Brown PC, Thurman RG, Belinsky SA, Kauffman FC. 1991. Effect of ally alcohol on xanthine dehydrogenase activity in the perfused rat liver. Toxicol Lett 58: 1-6
Lasini AF, Pompella A, Comporti M. 1985. Liver glutathione depletion induced by bromobenzene, iodobenzene and diethymalate poisoning and its relation to lipid peroxidation and necrosis. Am J Pathol 118: 225-537
Richie JP, Yvonne L, Saudhamini P, Virgina Malloy NO, Jay AX. 1994. Methionine reaction on increases blood glutathione and longevity in F344 rats. FASEB J 8: 1302- 1307
Fernandez-Checa JC, Ookhtens M, Kapowia N. 1987. Effect of chronic ethanol feeding on rat hepatocytic glutathione compartmentation, frrlux, and response to incubation with ethanol. J Clin Invest 80: 57-62
Speisky H, Macdonald A, Giles G, Orrego H, Israd Y. 1985. Increased loss and decreased synthesis of hepatic glutathione after acute ethanol administration. Boichem J 225: 565-572
Aykac C, Uysal M, Yalcin S, Kocak-Tokar N, Sivas A, Oz H. 1985. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicol 36: 71-76
Videla LA, Fernandez V, Valenzuela A, Ugarte G. 1981. The effects of chronic alcohol ingestion on free radical defense in the immature pig. Pharmacology 22: 343-348
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