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LPS로 자극한 RAW264.7 대식세포주에서 회향 추출물에 의한 염증성 매개물의 생성 억제
Inhibition of lipopolysaccharide-stimulated inflammatory mediator production in RAW264.7 macrophages by Foeniculum vulgare fruit extract 원문보기

한국조리과학회지 = Korean Journal of Food and Cookery Science, v.20 no.5, 2004년, pp.505 - 510  

최은미 (경희대학교 식품영양학과) ,  구성자 (경희대학교 식품영양학과)

초록
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이물질 침입에 대한 인식의 결과 NO, PGE$_2$, TNF-, IL-6와 같은 여러 신호전달물질의 분비가 개시되며 이들을 억제하는 물질을 항염증제라고 볼 수 있다. 본 연구에서는 회향(Foeniculum vulgare Mill.) 열매 추출물이 mouse macrophages RAW264.7 세포에서 lipopolysaccharide(LPS)로 유도한 NO(iNOS 산물), PGE$_2$(COX-2 산물) 및 cytokines (TNF-$\alpha$, IL-6) 생성 억제에 미치는 영향을 살펴보았다. 회향 열매의 methanol 추출물 및 분획물(chloroform, butanol, and aqueous fractions)은 4～100$\mu$g/mL 농도에서 LPS가 활성화된 대식 세포에서 NO 생성을 억제하였으며 독성을 나타내지 않았다. LPS가 유도한 PGE$_2$ 생성은 butanol 분획(100 $\mu$g/mL)에 의해서만 유의적으로 감소하였다(P<0.05). 회향 열매 추출물 및 분획물은 TNF-$\alpha$의 생성을 유의적으로 감소시켰으며 IL-6의 생성은 methanol extract(4～100 $\mu$g/mL), chloroform fraction(4 $\mu$g/mL), butanol fraction(4 and 100$\mu$g/mL) 및 aqueous fraction(4～100 $\mu$g/mL)에 의해 감소되었다(P<0.05). 이는 회향 열매 추출물은 염증 상태에서 유용할 것이며 COX-2와 iNOS를 억제하는 butanol 분획은 새로운 항염증제 개발에 사용될 수 있음을 시사하여 주었다.

주제어

#Foeniculum vulgare   #nitric oxide   #prostaglandin   #cytokin  

AI 본문요약
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제안 방법

  • In this study, Foeniculum vulgare fruit extract and its fractions (chlorofonn, butanol and aqueous fractions) were evaluated with the LPS-induced PGE2 accumulation (for COX-2 inhibitors) system in cultured RAW264.7 cells. As shown in Table 1, butanol fraction( lOOg/mL) showed significant inhibitory activity on COX-2, but other fractions did not inhibit COX-2 activity (p<0.
  • In the course of our biological search for anti-inflammatory agents, we conducted a study using alcoholic extract from Foeniculum vulgare fruit and its subfractions (chloroform, butanol, and aqueous fractions) obtained bythe solvent extraction to separate the components according to their polarities. In this study, the ability of Foeniculum vulgare fruit extract and its fractions to modulate the production of some inflammatory mediators (NO, PGE2, TNF- a and IL-6) were investigated in mouse macrophage cell line RAW264.7.
  • After additional 16 h incubation, the media were removed and analyzed by PGE2 enzyme immunometric assay kit (R&D System, USA) according to the manufacturer's instructions. The assay is based on competition between unlabelled PGE2 and a fixed quantity of peroxidase-labelled PGE2 for a limited number of binding sites on a PGE2 specific antibody. Percent inhibition was expressed as 100X[1 -(PGE2 release with sample-spontaneous release)/ (PGE2 release without sample-spontaneous release)].

데이터처리

  • The results were expressed as mean ± SEM. Statistical analysis was performed using ANOVA followed by Dunnetfs t-test (p<0.05). The analysis was performed using SAS statistical software.

이론/모형

  • To assess the effect of Foeniculum vulgare fruitextract on LPS-induced NO production (for iNOS inhibitors) in RAW264.7 macrophages, cell culture medium was harvested, and the concentration of accumulated nitrite, the oxidative product of NO, was determined by the Griess method (Table 1).The addition of Foeniculum vulgare fruit extract and its fractions (chloroform, butanol and aqueous fractions) to cells that had been stimulated with LPS for 12 hr to induce iNOS activity inhibited the induced iNOS activity as evidenced by nitrite formation.
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참고문헌 (20)

  1. Bandaru, SR, Chinthalapally, VR and Karen, S : Evaluation of C0X-2 inhibitor for potential chemopreventive properties in colon carcinogenesis. Cancer Research 56: 4566-4569, 1996 

  2. Bertolini, A, Ottani, A and Sandrini, M : Dual acting anti-inflammatory drugs: a reappraisal. Pharmacol. Res., 44: 437-450, 2001 

    상세보기 crossref

  3. Bowie, A and O'Neill, LAJ : Oxidative stress and nuclear factor-kB activation: a reassessment of the evidence in the light of recent discoveries. Biochem. Pharmacol., 59: 13-23, 2000 

    상세보기 crossref

  4. Ding, A, Nathan, CF, Graycer, J, Derynck, R, Stuehr, DJ and Srimal, S : Macrophage deactivating factor and transforming growth factors- $\beta$ /1, - $\beta$ 2 and -/ $\beta$ 3 inhibit induction of macrophage nitrogen oxide synthesis by IFN- $\gamma$ . Journal of Immunology, 145: 940-944, 1990 

  5. Diterich, I, Harter, L, Hassler, D, Wendel, A and Hartung, T : Modulation of cytokine release in ex vivo-stimulated blood from borreliosis patients, Infect, hmmun., 69: 687-694, 2001 

    상세보기 crossref

  6. Durum, SK and Oppenheim, JJ : Macrophage-derived mediator: interleukin 1, tumor necrosis factor, inteleukin 6, interferon, and related cytokines. In: Paul WE (Ed.), Fundamental Immunology, $2^n^d$ ed., Raven Press Ltd., New York. 1989 

  7. Elsasser-Beile, U, von Klerst, S, Fischer, R, Martin, M, Wetterauer, U, Gallati, H and Monting, JS : Impared cytokine production in whole blood cell cultures of patients with urological carcinomas. J. Cancer Res. Clin. Oncol., 119: 430-433, 1993 

    상세보기 crossref

  8. Ertel, W, Krombach, F, Kremer, JP, Jarrar, D, Thiele, V, Eymann, J, Muenzing, S, Faist, E, Messmer, K and Schildberg, FW : Mechanisms of cytokine transcription despite reduced CD14 receptor expression. Surgery, 114: 243-250, 1993 

  9. Evans, CH : Nitric oxide: what role does it play in inflammation and tissue destruction? Agents Actions Suppl, 47: 107-16, 1995 

  10. Guillen, MD and Manzanos, MJ : A contribution to study Spanish wild grown fennel (FoenicuIum vulgare Mill.) as a source of flavor compounds. Chem. Mikrobiol. Technol. Lehensm., 16: 141-145, 1994 

  11. Hartung, T, Pitrak, DL, Foote, M, Shatzen, EM, Verral, SC and Wendel, A : Filgrastim restores interleukin-2 production in blood from patients with advanced human immunodeficiency virus infection. J. Infect. Dis., 178: 686-692, 1998 

    상세보기 crossref

  12. Hinz, B and Brune, K : Specific C0X-2 inhibitors: prospects of therapy with new analgesic and anti-inflammatory substances. Wien. Klin. Wochenschr., Ill: 103-112, 1999 

  13. Hong, CH, Hur, SK, Oh, OJ, Kim, SS, Nam, KA and Lee, SK : Evaluation of natural products on inhibition of inducible cyclooxygenase (C0X-2) and nitric oxide synthase (iNOS) in cultured mouse macrophage cells Journal of Ethnopharmacology, 83: 153-159, 2002 

    상세보기 crossref

  14. Li, N and Karin, M: Is NF-kB the sensor of oxidative stress. FASEB J., 13: 1137-1143, 1998 

  15. Nathan, C : Nitric oxide as a secretory product of mammalian cells FAEB J., 6: 3051-64, 1992 

  16. Toda, S : Inhibitory effects of aromatic herbs on lipid peroxidation and protein oxidative modification by copper. Phytother. Res., 17(5): 546-8, 2003 

    상세보기 crossref

  17. Tsao, LT, Lee, CY, Huang, LJ, Kuo, SC and Wang, JP : Inhibition of lipopolysaccharide-stimulated nitric oxide production in RAW264.7 macrophages by asynthetic carbazole, LCY-2-CH0. Biochemical Pharmacology, 7254: 1-8, 2002 

  18. Tubaro, A, Florio, C, Luxich, E, Vertua, R, Loggia, RD and Yasumoto, T : Suitability of the MTT-based cytotoxicity assay to detect okadaic acid contamination of mussels. Toxicon, 34: 965-74, 1996 

    상세보기 crossref

  19. Yamini, Y, Sefidkon, F andPourmortazavi, SM : Comparison of essential oil composition of Iranian fennel (Foeniculum vulgare) obtained by supercritical carbon dioxide extraction and hydrodistillation methods. Flavour. Fragr. J., 17: 345-348, 2002 

    상세보기 crossref

  20. Zangerle, PF, De Groote, D, Lopez, M, Meuleman, RJ, Vrindts,Y, Fauchet, F, Dehart, I, Jadoul, M, Radoux, D and Franchimont, P : Direct stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood: II. Application to rheumatoid arthritis and osteoarthritis. Cytokine, 4: 568-575, 1992 

    상세보기 crossref

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