수생식물 개구리밥(Spirodela polyrhiza)의 식물체 발달 과정에서 나타나는 미생물과의 상호관계를 주사전자현미경 및 세포학적으로 추적하여 연구하였다. 개구리밥은 부유성 식물로 분화 발달 초기부터 잠수부위에 여러 종류의 박테리아, 남조류, 규조류 등이 서식하였다. 미분화된 어린 식물체에는 성숙 발달한 식물체에 비해 미생물들이 낮은 빈도로 출현하였고, 성숙한 식물체에서는 특히 엽상체하피에 가장 많은 미생물들이 서식하였으며, 그 다음으로는 점액성 물질이 층을 이루는 근관에 많이 관찰되었다. 반면, 신장 발달 중의 뿌리에는 일반적인 분포를 보였으며, 상피에는 미생물이 거의 분포하지 않는 양상을 보였다. 이들 미생물이 개구리밥 조직 내에 침입하여 식물체에 어떠한 영향을 미치는가를 조사하기 위해 엽상체, 연결사, 뿌리, 근관, 무성생식낭 등의 구조를 세포학적으로 추적한 결과, 어느 부위에서도 조직 내로 미생물이 침입하여 세포를 괴사시키거나 감염시키는 일은 거의 관찰되지 않았다. 그러므로, 개구리밥 식물체의 발달과정에 있어 미생물들은 어떠한 해를 주지 않는 것으로 추정된다. 이후 개구리밥에 서식하는 미생물의 질소고정치를 측정하여 그 기능을 식물체의 세포학적 측면과 접목시켜 연구하면 개구리밥과 미생물과의 상호관계가 공생적인지를 더 확실하게 밝힐 수 있을 것이다.
수생식물 개구리밥(Spirodela polyrhiza)의 식물체 발달 과정에서 나타나는 미생물과의 상호관계를 주사전자현미경 및 세포학적으로 추적하여 연구하였다. 개구리밥은 부유성 식물로 분화 발달 초기부터 잠수부위에 여러 종류의 박테리아, 남조류, 규조류 등이 서식하였다. 미분화된 어린 식물체에는 성숙 발달한 식물체에 비해 미생물들이 낮은 빈도로 출현하였고, 성숙한 식물체에서는 특히 엽상체 하피에 가장 많은 미생물들이 서식하였으며, 그 다음으로는 점액성 물질이 층을 이루는 근관에 많이 관찰되었다. 반면, 신장 발달 중의 뿌리에는 일반적인 분포를 보였으며, 상피에는 미생물이 거의 분포하지 않는 양상을 보였다. 이들 미생물이 개구리밥 조직 내에 침입하여 식물체에 어떠한 영향을 미치는가를 조사하기 위해 엽상체, 연결사, 뿌리, 근관, 무성생식낭 등의 구조를 세포학적으로 추적한 결과, 어느 부위에서도 조직 내로 미생물이 침입하여 세포를 괴사시키거나 감염시키는 일은 거의 관찰되지 않았다. 그러므로, 개구리밥 식물체의 발달과정에 있어 미생물들은 어떠한 해를 주지 않는 것으로 추정된다. 이후 개구리밥에 서식하는 미생물의 질소고정치를 측정하여 그 기능을 식물체의 세포학적 측면과 접목시켜 연구하면 개구리밥과 미생물과의 상호관계가 공생적인지를 더 확실하게 밝힐 수 있을 것이다.
Fresh specimens of the aquatic macrophyte, Spirodela polyrhiza, have been examined employing scanning and transmission electron microscopy. Observations revealed the occurrence of microbial colonization during development. Submerged parts of the small, free-floating S. polyrhiza body exhibited a var...
Fresh specimens of the aquatic macrophyte, Spirodela polyrhiza, have been examined employing scanning and transmission electron microscopy. Observations revealed the occurrence of microbial colonization during development. Submerged parts of the small, free-floating S. polyrhiza body exhibited a variety of microorganisms such as bacteria, cyanobacteria, and diatoms throughout their development. However, immature and/or young plants normally demonstrated much less microbial colonization compared to mature plants. During the study, heavy colonization by the microorganisms was routinely encountered at maturity, especially in the fully developed abaxial fronds and root caps. The mucilaginous layer was shown along the root caps, and the microorganisms appeared to be either clustered or attached to this layer. In contrast, only moderate degrees of colonization were observed in the root, and little to no colonization was observable in the adaxial frond surface. Transmission electron microscopy clearly demonstrated the microbial colonization to be external in the S. polyrhiza specimen examined in the current study. The association between the microorganisms and S. polyrhiza has been considered non-harmful, as no frond senescence and almost no mechanical penetration of the plant by the microorganisms were noticed during the study.
Fresh specimens of the aquatic macrophyte, Spirodela polyrhiza, have been examined employing scanning and transmission electron microscopy. Observations revealed the occurrence of microbial colonization during development. Submerged parts of the small, free-floating S. polyrhiza body exhibited a variety of microorganisms such as bacteria, cyanobacteria, and diatoms throughout their development. However, immature and/or young plants normally demonstrated much less microbial colonization compared to mature plants. During the study, heavy colonization by the microorganisms was routinely encountered at maturity, especially in the fully developed abaxial fronds and root caps. The mucilaginous layer was shown along the root caps, and the microorganisms appeared to be either clustered or attached to this layer. In contrast, only moderate degrees of colonization were observed in the root, and little to no colonization was observable in the adaxial frond surface. Transmission electron microscopy clearly demonstrated the microbial colonization to be external in the S. polyrhiza specimen examined in the current study. The association between the microorganisms and S. polyrhiza has been considered non-harmful, as no frond senescence and almost no mechanical penetration of the plant by the microorganisms were noticed during the study.
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문제 정의
The entire plant body, including the fronds, connective stalks, roots, root caps, and reproductive pockets of young and mature duckweeds were examined by using both scan ning and transmission electron microscopy. The study foc uses on the relationship between the giant duckweed and microbial colonization within their environment. The work presented lias been carried out as part of a larger study on the structural aspects of the giant duckweed in regards to the structural and cellular aspects of S.
제안 방법
period. The entire plant body, including the fronds, connective stalks, roots, root caps, and reproductive pockets of young and mature duckweeds were examined by using both scan ning and transmission electron microscopy. The study foc uses on the relationship between the giant duckweed and microbial colonization within their environment.
성능/효과
1. Plant material : About 50 ~60 fresh specimens of Spirodsla polyrhiza (L.) Sclileiden were collected from Woopo Marsh, Changryoung; Kyungbook Province, during the years 2002 and 2003. After being transported from the collection sites, approximately 20 plants with at least two to three generations of offspring fronds con nected to mother fronds with healthy stalks were select ed for the following transmission and scanning electron microscopy.
참고문헌 (26)
Baker JH, Farr IS: Organic secretions by duckweed and their relationship with epiphytic bacteria. In Rhodes Roberts M, Skinner FA, eds, Bacteria and Plants. Soc Appl Bact Symp Ser No 10, p. 250, 1982
Baker JH, Orr DR: Distribution of epiphytic bacteria on fresh water plants. J Ecol 74 : 155 165, 1986
Echlin P, Lai CE, Hayes TL: The distribution and relative concentration of potassium in the root tips of Lemna minor analyzed using low temperature x ray microanalysis L. Scan Electron Microsc 2 : 489 498, 1981
Echlin P, Lai CE, Hayes TL: Low temperature X ray microanalysis of the differentiating vascular tissue in root tips of Lemna minor L. J Microsc 126 : 285 306, 1982
Echlin P, Lai CE, Hayes TL. Hook G: Elemental analysis of frozen hydrated differentiating phloem parenchyma in roots of Lemna minor L. Scan Electron Microsc 2 : 383 394, 1980
Echlin P, Pawley JB, Hayes TL: Freeze fracture scanning electron microscopy of Lemna minor L. Scan Electron Microsc 3 : 69 76, 1979
Federle TW, Schwab BS: Mineralization of surfactants by microbiota of aquatic plants. Appl Environ Microbiol 8 : 2092 2094, 1989
Hossel JC, Baker JH: Estimation of the growth rates of epiphytic bacteria and Lemna minor in a river. Freshwater BioI 9 : 319 327, 1979
Ji SY, Kim IS: Structural features of various trichomes developed in Salvinia natans. Kor J Electron Microsc 32 : 319 327, 2002
Kim KA, Kim IS: Structural aspects of the reduced free floating hydrophyte, Spirodela polyrhiza. Kor J Electron Microsc 30 : 233 240, 2000
Landolt E: Anatomy of the Lemnaceae (duckweeds). In: Landolt E, Jager Zum I, Schnel RAAl, eds, Extreme Adaptations in Angiospermous Hydrophytes. pp. 1 127, Borntraeger, Berlin, 1998
Lemon GD, Posluszny U: Comparative shoot development and evolution in the Lemnaceae. Intl J Plant Sci 161 : 733 748, 2000
Muhonen M, Showman J, Couch R: Nutrient absorption by Spirodela polyrhiza. J Aquat Plant Manage 21 : 107 109, 1983
Pedersen O, Sand Jensen K: Water transport in submerged macrophytes. Aquat Bot 47 : 155 174, 1993
Rimes CA, Goulder R: Temporal variation in density of epiphytic bacteria on submerged vegetation in a calcareous stream. Lett AppI Micro 3 : 17 21, 1986
Underwood GJC, Baker JH: The effect of various aquatic bacteria on the growth and senescence of duckweed (Lemna minor). J Appl Bacteriol 70 : 192 196, 1991
Zuberer DA: Nitrogen fixation (Acetylene reduction) associated with duckweed (Lemnaceae) mats. Appl Environ Micro 43 : 823 828, 1982
Zuberer DA: Microbial colonization of some duckweeds (Lemnaceae): examination by scanning and transmission electron and light microscopy. Aquat Bot 18 : 275 285, 1984
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