나노 세라마이드의 캡슐화(Nano-ceramide capsulation)는 세라마이드 III와 토코페릴리놀레이트를 나노 크기의 모노 베지클에 캡슐화시켜 피부각질층에 작용하도록 만든 기술이다. 그 제조에는 고압 마이크로플루다이저를 사용하였으며 조성은 다음과 같다. 모노 베지클의 막 강화제로서 $0.5{\~}5.0\;wt\%의$ 수소첨가레시친과 $0.1{\~}2.0\;wt\%의$ 리소레시친을 사용하였으며, 용제로서 $5.0{\~}10.0\;wt\%의$ propylene glycol과 $5.0{\~}10.0\;wt\%의$ ethanol을 사용하였다. 피부 보습기능과 아토피 치료를 위하여 활성성분인 세라마이드 III와 토코페릴리놀레이트를 사용하였으며 유화제는 함유하지 않았다. 나노 세라마이드 캡슐기술의 최적조건은 다음과 같다. 마이크로플루다이저의 통과압력은 1,000 bar, 통과횟수는 3회, 통과 온도는 $60{\~}70^{\circ}C가$ 적당하였다. 또한, nano capsule의 pH는 $5.8\pm0.5이었다.$ 평균입자크기는 $63.1{\pm}7.34 nm로$ 물과 같은 투명한 성상을 보였으며, 제타포텐셜값은 $-55.1{\pm}0.84mV이었다.$임상실험 결과로서, 피부보습효과(in-vivo, n=8, p-value<0.05)는 비교시료보다 $21.15{\%}$ 개선되었으며, 치료 전보다는 $36.31{\%}$ 개선되었다. 더구나, 아토피 피부 효과는 아토피 피부 환자 10명에게서 양성반응을 보였다.
나노 세라마이드의 캡슐화(Nano-ceramide capsulation)는 세라마이드 III와 토코페릴리놀레이트를 나노 크기의 모노 베지클에 캡슐화시켜 피부각질층에 작용하도록 만든 기술이다. 그 제조에는 고압 마이크로플루다이저를 사용하였으며 조성은 다음과 같다. 모노 베지클의 막 강화제로서 $0.5{\~}5.0\;wt\%의$ 수소첨가레시친과 $0.1{\~}2.0\;wt\%의$ 리소레시친을 사용하였으며, 용제로서 $5.0{\~}10.0\;wt\%의$ propylene glycol과 $5.0{\~}10.0\;wt\%의$ ethanol을 사용하였다. 피부 보습기능과 아토피 치료를 위하여 활성성분인 세라마이드 III와 토코페릴리놀레이트를 사용하였으며 유화제는 함유하지 않았다. 나노 세라마이드 캡슐기술의 최적조건은 다음과 같다. 마이크로플루다이저의 통과압력은 1,000 bar, 통과횟수는 3회, 통과 온도는 $60{\~}70^{\circ}C가$ 적당하였다. 또한, nano capsule의 pH는 $5.8\pm0.5이었다.$ 평균입자크기는 $63.1{\pm}7.34 nm로$ 물과 같은 투명한 성상을 보였으며, 제타포텐셜값은 $-55.1{\pm}0.84mV이었다.$ 임상실험 결과로서, 피부보습효과(in-vivo, n=8, p-value<0.05)는 비교시료보다 $21.15{\%}$ 개선되었으며, 치료 전보다는 $36.31{\%}$ 개선되었다. 더구나, 아토피 피부 효과는 아토피 피부 환자 10명에게서 양성반응을 보였다.
The nano-ceramide capsulation is a technique that capsulates ceramide III and tocopheryl linoleate at the mono-vesicle to act on the horny layer in skin. In this technique, $0.5{\~}5.0\;wt\%$ of hydrogenated lecithin and $0.01{\~}2.00\;wt\%$ of lysolecithin are used as the memb...
The nano-ceramide capsulation is a technique that capsulates ceramide III and tocopheryl linoleate at the mono-vesicle to act on the horny layer in skin. In this technique, $0.5{\~}5.0\;wt\%$ of hydrogenated lecithin and $0.01{\~}2.00\;wt\%$ of lysolecithin are used as the membrane-strengthen agents of the mono-vesicle and $5.0{\~}10.0\;wt\%$ of propylene glycol and $5.0{\~}10.0\;wt\%$ of ethyl alcohol are used as solvents. Active ingredients such ceramide III and tocopheryl linoleate are utilized to enhance the moisturizing efficacy and treat atopy skin. These materials do not contain synthetic emulsifiers. The optimal conditions or nano-ceramide capsulation are such that particles pass Microfludizdizer 3 times at 1,000 bar and $60{\~}70^{\circ}C$ and pH of nano capsules is $5.8{\pm}0.5.$ The average size of particles is $63.1{\pm}7.34\;nm$ showing lucid state like water by the laser light scattering. A zeta potential value is $-55.1\pm0.84\;mV.$ Through clinical tests, the moisturizing effect (in-vivo, n=8, p-value<0.05) showed $21.15\%$ of improvement comparison to comparison-samples and $36.31\%$ of improvement compared to the state before treatment. Moreover, the effectiveness of atopy skin showed positive reaction from 10 volunteers.
The nano-ceramide capsulation is a technique that capsulates ceramide III and tocopheryl linoleate at the mono-vesicle to act on the horny layer in skin. In this technique, $0.5{\~}5.0\;wt\%$ of hydrogenated lecithin and $0.01{\~}2.00\;wt\%$ of lysolecithin are used as the membrane-strengthen agents of the mono-vesicle and $5.0{\~}10.0\;wt\%$ of propylene glycol and $5.0{\~}10.0\;wt\%$ of ethyl alcohol are used as solvents. Active ingredients such ceramide III and tocopheryl linoleate are utilized to enhance the moisturizing efficacy and treat atopy skin. These materials do not contain synthetic emulsifiers. The optimal conditions or nano-ceramide capsulation are such that particles pass Microfludizdizer 3 times at 1,000 bar and $60{\~}70^{\circ}C$ and pH of nano capsules is $5.8{\pm}0.5.$ The average size of particles is $63.1{\pm}7.34\;nm$ showing lucid state like water by the laser light scattering. A zeta potential value is $-55.1\pm0.84\;mV.$ Through clinical tests, the moisturizing effect (in-vivo, n=8, p-value<0.05) showed $21.15\%$ of improvement comparison to comparison-samples and $36.31\%$ of improvement compared to the state before treatment. Moreover, the effectiveness of atopy skin showed positive reaction from 10 volunteers.
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가설 설정
Protecting homy layer by generally treatment method, and must use moisturizer that is effective in hydration of skin[12]. Therefore, this study studied about nano capsulation and effect to act more effectively to atopy skin. It established optimum condition that make mono vesicle using ceramide and tocopheryl linoleate by main ingre dients.
제안 방법
Results are the average value of five measurements at the stationary level. The experiments were located in a constant temperature and humidity room for 20 min prior to moisture evalu ation to minimize effect of atmospheric conditions on the measurement. Comeometer (CM820PC, Courage Khazka, Germany) was used to measure the moisture.
To investigate the moisture on the patients with atopic dermatitis were used in-vivo test. The moisture values on the affect part of patients with the atopic dermatitis were evaluated using the seven-point scales and then the affect parts were took the photographs. By using the seven-point scales the improvement effect of the sample revealed from 2.
The improvable effects on atopy skin were measured on the 10 volunteers. The sample was applied on the part of affected skin for the experiment twice a day in morning and evening after washing. The measurements for the affected part of atopy skin were made before applying the sanx)le, and then after applying the sample were made using the seven-point scales with the naked eyes of an expert on the 28th days.
대상 데이터
The experiments were used 10 healthy 20s women in order to measure the skin moisture. The experiments were located in a constant temperature and humidity room for 20 min before moisture measurement to min imize the effect of the temperature and humidity.
The improvable effects on atopy skin were measured on the 10 volunteers. The sample was applied on the part of affected skin for the experiment twice a day in morning and evening after washing.
성능/효과
1. The optimal concentrations of the nano capsule were 0.50 wt% for saturated lecithin, 0.50 wt% for unsaturated lecithin and 2.00 wt% for lysolecithin, respectively.
2. At below pH 4 the particle size increased and the turbidity revealed a cloudy, and at any other pH 4 the particle size and the turbidity did not show any changes. The variation of pH did not affect the refractive index and the stability.
4. By using the seven-point scales, the capacitance of moisture increased from 2.7 to hence the improvement effect on the atopy skin through the nano capsule could be confirmed.
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