본 연구는 화장품에서 사용되는 다양한 자극원에 대한 자극완화제로서의 세라마이드를 구성성분으로 하는 나노리포좀의 잠재적 가능성을 알아보고자 하였다. 세라마이드는 인체로부터 수분 손실을 막고, 외부의 물리적, 화학적, 그리고 미생물에 의한 손상으로부터 신체를 보호함으로써 인간의 생리작용에 있어 중요한 부분을 담당하는 것으로 알려진 표피 투과 장벽의 주요한 구조적 구성 성분이다. 본 연구 결과에 의하면 피부 장벽 기능 강화와 자극완화 효과가 제형 내에 단순히 분산된 세라마이드보다 세라마이드를 구성성분으로 하는 나노리포좀을 함유하는 경우 보다 우수하게 나타났다. 그리고, 자극원으로서 제형 내 함유되어 있는 젖산의 피부 투과도에 있어서 세라마이드를 구성성분으로 하는 나노리포좀의 영향을 평가하기 위해 무모 생쥐에서 얻어낸 피부 막으로 horizontal franz diffusion cells을 이용한 in vitro 피부 투과 시험을 수행해 보았다. 시험 결과, 세라마이드로 구성되 나노리포좀의 항자극 효과는 자극원의 피부 투과도를 감소시키는 것으로 확인하였다. 결론적으로, 본 연구에서는 새로운 자극완화 시스템의 개발이 가능하였고 이러한 세라마이드를 구성성분으로 하는 나노리포좀을 화장품에 적용 가능하였다.
본 연구는 화장품에서 사용되는 다양한 자극원에 대한 자극완화제로서의 세라마이드를 구성성분으로 하는 나노리포좀의 잠재적 가능성을 알아보고자 하였다. 세라마이드는 인체로부터 수분 손실을 막고, 외부의 물리적, 화학적, 그리고 미생물에 의한 손상으로부터 신체를 보호함으로써 인간의 생리작용에 있어 중요한 부분을 담당하는 것으로 알려진 표피 투과 장벽의 주요한 구조적 구성 성분이다. 본 연구 결과에 의하면 피부 장벽 기능 강화와 자극완화 효과가 제형 내에 단순히 분산된 세라마이드보다 세라마이드를 구성성분으로 하는 나노리포좀을 함유하는 경우 보다 우수하게 나타났다. 그리고, 자극원으로서 제형 내 함유되어 있는 젖산의 피부 투과도에 있어서 세라마이드를 구성성분으로 하는 나노리포좀의 영향을 평가하기 위해 무모 생쥐에서 얻어낸 피부 막으로 horizontal franz diffusion cells을 이용한 in vitro 피부 투과 시험을 수행해 보았다. 시험 결과, 세라마이드로 구성되 나노리포좀의 항자극 효과는 자극원의 피부 투과도를 감소시키는 것으로 확인하였다. 결론적으로, 본 연구에서는 새로운 자극완화 시스템의 개발이 가능하였고 이러한 세라마이드를 구성성분으로 하는 나노리포좀을 화장품에 적용 가능하였다.
The objective of this study is to suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics. Ceramides are major structural components of the epidermal permeability barrier, which is known to play an essential part in human physio...
The objective of this study is to suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics. Ceramides are major structural components of the epidermal permeability barrier, which is known to play an essential part in human physiology by not only preventing the loss of water from the body but also protecting the body from external physical, chemical, and microbial insults. According to the results, better effects on reinforcement of skin barrier function and anti-irritation were obtained with nanoliposome composed of ceramide than with dispersed ceramide. And, we performed in vitro skin penetration test using horizontal Franz diffusion cells with skin membrane prepared from hairless mouse to evaluate the influence of nanoliposome composed of ceramide on the skin penetration of lactic acid in formulations. From the results, we found that the anti-irritation effects of nanoliposome containing ceramide were due to reduced penetration rate of irritants. Conclusively, we could develop a new anti-irritation system and apply this nanoliposome composed of ceramide to the final cosmetic products successfully.
The objective of this study is to suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics. Ceramides are major structural components of the epidermal permeability barrier, which is known to play an essential part in human physiology by not only preventing the loss of water from the body but also protecting the body from external physical, chemical, and microbial insults. According to the results, better effects on reinforcement of skin barrier function and anti-irritation were obtained with nanoliposome composed of ceramide than with dispersed ceramide. And, we performed in vitro skin penetration test using horizontal Franz diffusion cells with skin membrane prepared from hairless mouse to evaluate the influence of nanoliposome composed of ceramide on the skin penetration of lactic acid in formulations. From the results, we found that the anti-irritation effects of nanoliposome containing ceramide were due to reduced penetration rate of irritants. Conclusively, we could develop a new anti-irritation system and apply this nanoliposome composed of ceramide to the final cosmetic products successfully.
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제안 방법
However, there have been few reports describing the possibilities of these structural lipids as anti-irritants in cosmetics. In this study we compared the anti-irritation effects of formulas containing nanoliposome composed of ceramide with those containing dispersed ceramide and placebo. To investigate the potency of nanoliposome com posed of ceramide for reinforcement of skin barrier function, we examined the recovery rate of transepidermal water loss (TEWL) after tape stripping and the inhibitory effect of flush induced by methyl nicotinate.
Written informed consent was obtained from all participants. Measurements were performed, according to the guidelines from the standardization group of contact dermatitis[ll]z at time 0- before (baseline TEWL) and at just after tape stripping. For tape stripping, adhesive tapes(3M) were applied with gentle pressure to the test areas of forearm and then removed.
In this study we compared the anti-irritation effects of formulas containing nanoliposome composed of ceramide with those containing dispersed ceramide and placebo. To investigate the potency of nanoliposome com posed of ceramide for reinforcement of skin barrier function, we examined the recovery rate of transepidermal water loss (TEWL) after tape stripping and the inhibitory effect of flush induced by methyl nicotinate. And, clinical studies such as human patch test and stinging potential test were conducted to evaluate the anti-irritation effects of nanolipo- some composed of ceramide using lactic acid as a chemical insult.
대상 데이터
This parameter evaluates the water loss in g/㎡/h, measured using a Tewameter TM 210 (Courage & Khazaka, Germany). A total of 10 healthy volunteers without past or present of skin diseases (1 male, 9 females, mean age 26 years, range 23—30) were included in the study. Written informed consent was obtained from all participants.
It is known that topically applied methyl nicotinate leads to a generalized cutaneous erythema(flush) mediated at least in part by prostaglandin biosynthesis[13]. Ten healthy volunteers aged 23 — 30(1 male, 9 females, mean age 26 years) were included in the study. They did not receive any anti-inflammatory drug for 10 days prior to the study.
). The UV detector was set at 210 nm. A Mightsil Cl8 column (250 nun length, 4.
We performed human patch test to evaluate primary skin irritation potential according to the method of CTFA safety testing guidelines[15] with a slight modification. Twenty human volunteers (eight women and twelve men), free from skin disease or allergy, participated in the test. Their age ranged 23 to 35 years (average 28).
Those who reported stinging for 3 to 5 min within the first 8 min were designated as stingers and used for subsequent tests. Twenty human volunteers (ten women and ten men) participated in this test as stingers. Their age ranged 23 to 32 years (average 28).
성능/효과
From the results, we could found that a formula con taining nanoliposome composed of ceramide had excellent effects on reinforcement of skin barrier function and anti irritation compared to placebo and formula containing dis persed ceramide. Conclusively, we suggest the potentialities of nanoliposome composed of ceramide as an anti-irritant against various irritants used in cosmetics.
참고문헌 (15)
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Lachapelle, J. M. 1992. Textbook of Contact Dermatitis. London: Springer-Verlag
Lammintausta, K., H. I. Maibach and D. Wilson. 1988. Mechanisms of subjective (sensory) irritation: Propensity of non-immunologic contact urticaria and objective irritation in stingers. Dermatosen in Beruf und Umwelt 36, 45-49
Lampe, M. A., A. L. Burlingame, J. Whitney, M. I. Williams, B. E. Brown, E. Roitman and P. M. Elias. 1983. Human stratum corneum lipids: Characterization and regional variations. J. Lipid Res. 24, 120-130
Pinnagoda, J., R. A Tupker, T. Agner and J. Serup. 1990. Guidelines for transepidermal water loss measurement. A report from the standardization group of the European Society of Contact Dermatitis. Contact Dermatitis 22, 164-178
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