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논문 상세정보

Abstract

Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY188753) consisted of an open reading frame (ORP) of 1,116 bp encoding 371 amino acid residues with a signal peptide of 22 amino acids within the NH$_2$-terminal region of pre-CelC. The predicted amino acid sequence of CelC was similar to that of Peetobaeterium ehrysanthemi Cel8Y (AF282321). The CelC has the conserved region of the glycoside hydrolase family 8. The apparent molecular mass of CelC was calculated to be 39 kDa by CMC-SDS-PAGE. The cellulase­minus mutant of Pee LY34 was as virulent as the wild-type in pathogenicity tests on tubers of potato. The results suggest that the CelC of Pce LY34 is a minor factor for the pathogenesis of soft-rot.

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이 논문을 인용한 문헌 (6)

  1. Hong, Su-Young ; Cho, Kye-Man ; Kim, Yong-Hee ; Hong, Sun-Joo ; Cho, Soo-Jeong ; Cho, Yong-Un ; Kim, Hoon ; Yun, Han-Dae 2006. "Cloning and Identification of Essential Residues for Thermostable β-glucosidase (BgIB) from Thermotoga maritima" 생명과학회지 = Journal of life science, 16(7): 1148~1157 
  2. 2006. "" Journal of microbiology and biotechnology, 16(8): 1325~1329 
  3. Woo, Sang-Min ; Kim, Sang-Dal 2007. "Cloning of the Cellulase Gene and Characterization of the Enzyme from a Plant Growth Promoting Rhizobacterium, Bacillus licheniformis K11" 한국응용생명화학회지 = Journal of the Korean Society for Applied Biological Chemistry, 50(2): 95~100 
  4. Cho, Kye-Man ; Kim, Eun-Ju ; Math, Renukaradhya K. ; Asraful Islam, Shah Md. ; Hong, Sun-Joo ; Kim, Jong-Ok ; Shin, Ki-Jae ; Lee, Young-Han ; Kim, Hoon ; Yun, Han-Dae 2007. "Cloning of Isoamylase Gene of Pectobacterium carotovorum subsp. carotovorum LY34 and Identification of Essential Residues of Enzyme" 생명과학회지 = Journal of life science, 17(9): 1182~1190 
  5. 2008. "" Journal of microbiology and biotechnology, 18(3): 404~409 
  6. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(1): 78~88 

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