A methanol soluble extract from the dried hooks and stems of Uncaria rhynchophylla showed a strong inhibitory activity against monoamine oxidase in mouse brain. Using a bioassay-guided purification of this extract, a known ${\beta}-carboline$ type alkaloid, harman (1), was obtained as an ...
A methanol soluble extract from the dried hooks and stems of Uncaria rhynchophylla showed a strong inhibitory activity against monoamine oxidase in mouse brain. Using a bioassay-guided purification of this extract, a known ${\beta}-carboline$ type alkaloid, harman (1), was obtained as an active constituent. In addition, five known indole alkaloids, isocorynoxeine (2), isorhynchophylline (3), corynoxeine (4), cadambine (5), and $3{\alpha}-dihydrocadambine$ (6), were isolated and found to be weakly active or inactive.
A methanol soluble extract from the dried hooks and stems of Uncaria rhynchophylla showed a strong inhibitory activity against monoamine oxidase in mouse brain. Using a bioassay-guided purification of this extract, a known ${\beta}-carboline$ type alkaloid, harman (1), was obtained as an active constituent. In addition, five known indole alkaloids, isocorynoxeine (2), isorhynchophylline (3), corynoxeine (4), cadambine (5), and $3{\alpha}-dihydrocadambine$ (6), were isolated and found to be weakly active or inactive.
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제안 방법
ELMS was measured on a Hewlett Packard 5989A mass spectrometer. Open column chromatography was performed using a silica gel (Kieselgel 60, 70-230 mesh, Merck), and thin layer chromatography (TLC) using a pre-coated silica gel 60 F254 (0.25 mm, Merck). The fluorescence intensities were measured on a Perkin Elmer LS50B fluorescence spectrophotometer.
대상 데이터
Optical rotations were determined on JASCO DIP-370 polarimeter at 25℃. IR spectra were taken on a JASCO FT/IR 300E spectrometer. UV spectra were obtained on a Milton Roy 3000 spectrometer.
The ICR male mice were purchased from Samyook Animal Center (Soowon, Korea) and maintained in accordance with the guidelines for animal care and use of laboratory animals, Chungbuk National University, Korea.
The dried hooks and stems of Uncaria rhynchophylla were purchased from an herbal drug store at Cheongju, Korea, in September 2003 and identified by Emeritus Prof Kyong Soon Lee, a plant taxonomist at Chungbuk National University. A voucher specimen (No.
이론/모형
, 2001). The MAO activity with kynuramine as a substrate was assayed by a modification of the fluorometric method of Kraml (Kraml, 1965; Ro et al, , 2001). The samples (50 |il) were added to 0.
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