$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"

과학기술 지식인프라 ScienceON

상세검색 다국어 입력
도움말도움말
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

MyON담기
내보내기

Expression of Hepatitis C Virus Structural Proteins in Saccharomyces cerevisiae

Journal of microbiology and biotechnology v.15 no.4 , 2005년, pp.767 - 771  
LEE JONG-SOO (BioLeaders Corporation ) ;  YU JUNG ( Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University ) ;  SHIN HYUN-JIN ( BioLeaders Corporation ) ;  KIM YOUNG-SANG ( Department of Biochemistry, Chungnam National University ) ;  AHN JEONG-KEUN ( Microbiology, College of Natural Sciences, Chungnam National University ) ;  LEE CHONG-KIL ( Department of Pharmacy, Chungbuk National University ) ;  POO HARYOUNG ( Proteome Research Lab., Korea Research Institute of Bioscience and Biotechnology ) ;  KIM CHUL-JOONG ( Laboratory of Infectious Disease, College of Veterinary Medicine, Chungnam National University)
Abstract

Expression in yeast may prove more amenable to generating large amounts of viral antigens for a vaccine candidate. We, therefore, cloned the gene encoding the Hepatitis C virus (HCV) structural proteins (C-El-E2, c740) fused in-frame with, and immediately 3' to, the chicken-lysozyme signal peptide (C-SIG) gene and under the control of the yeast glyceraldehyde-3-phosphate dehydrogenase gene promoter. In yeast, the HCV structural proteins were expressed in two different forms: a processed and a nonprocessed aggregated form. Biophysical characterization by sucrose linear gradient centrifugation revealed that both forms were present in the same fractions with a buoyant density of 1.127-1.176 g/$cm^3$. These findings suggest that the efficient synthesis of HCV structural proteins in yeast may be an important tool to study virus assembly and may lead to the development of an HCV vaccine.

주제어

#HCV structural proteins   #yeast expression   #vaccine  

저자의 다른 논문

SHIN HYUN-JIN(1) Kim, Young-Sang(17) Ahn, Jeong-Keun(8) Lee, Chong-Kil(70) Kim, Chul-Joong(22)

참고문헌 (20)

  1. Chan-Fook, C., W. R. Jiang, B. E. Clarke, N. Zitzmann, C. Maidens, J. A. McKeating, and J. M. Jones. 2000. Hepatitis C virus glycoprotein E2 binding to CD81: The role of E 1 E2 cleavage and protein glycosylation in bioactivity. Virology 273: 60-66 
  2. Charloteaux, B., L. Lins, H. Moereels, and R. Brasseur. 2002. Analysis of the C-terrninal membrane anchor domains of hepatitis C virus glycoproteins EI and E2: Toward a topological model. J. Virol. 76: 1944-1958 
  3. Choukhi, A., A. Pillez, H. Drobecq, C. Sergheraert, C. Wychowski, and J. Dubuisson. 1999. Characterization of aggregates of hepatitis C virus glycoproteins. J. Gen. Virol. 80: 3099-3107 
  4. Falcon, V., C. Garcia, M. C. Rosa, I. Menendez, J. Seoane, and J. M. Grillo. 1999. Ultrastructural and immunocytochemical evidences of core-particle formation in the methylotrophic Pichia pastoris yeast when expressing HCY structural proteins (core-El). Tissue Cell 31: 117-125 
  5. Hinnen, A., J. B. Hicks, and G R. Fink. 1992. Transformation of yeast. Biotechnology 24: 337-341 
  6. Yasui, K., T. Wakita, K. Tsukiyama-Kohara, S. Funahashi, M. Ichikawa, T. Kajita, D. Moradpour, J. R. Wands, and M. Kohara. 1998. The native form and maturation process of hepatitis C virus core protein. J. Virol. 72: 6048-6055 
  7. Matatsumoto, M., S. B. Hwang, K. S. Jeng, N. Zhu, and M. N. Lai. 1996. Homotypic interaction and multimerization of hepatitis C virus core protein. Virology 218: 43-51 
  8. Clayton, R. F., A. Owsianka, J. Aitken, S. Graham, D. Bhella, and A. H. Patel. 2002. Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C viruslike particles. J. Virol. 76: 7672- 7682 
  9. Santolini, E., G Migliaccio, and N. Monica 1994. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J. Virol. 68: 3631-3641 
  10. Dubuisson, J. 1999. Folding, assembly and subcellular localization of HCY glcoproteins. Curr. Top. Microbiol. Immunol. 242: 135-148 
  11. Kuroda, S., T. Otaka, T. Miyazaki, M. Nakao, and Y. Fujisawa. 1992. Hepatitis B virus envelope L protein particles. J. Biol. Chem. 267: 1953-1961 
  12. Acosta-Rivero, N., J. C. Alvarez-Obregon, A. Musacchio, V. Falcon, S. Duenas-Carrera, J. Marante, J. Menendez, and J. Morales. 2002. In vitro self-assembly HCV core virus-like particles induce a strong antibody immune response in sheep. Biochem. Biophys. Res. Commun. 290: 300-304 
  13. Bartenschlager, R. and V. Lohmann. 2000. Replication of hepatitis C virus. J. Gen. Virol. 81: 1631- 1648 
  14. Kang, H. A., M. S. Jung, W. K. Hong, J. H. Sohn, E. S. Choi, and S. K. Rhee. 1998. Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisae. J. Microbiol. Biotechnol. 8: 42-48 
  15. Shin, D. J., J. C. Park, H. B. Lee, S. B. Chen, and S. Bai. 1998. Construction of a secretory expression vector producing an ${\alpha}$-amylase of yeast, Schwanniomyces occidentalis in Saccharomyces. J. Microbiol. Biotechnol. 8: 625-630 
  16. Op De Beeck, A., L. Cocquerel, and J. Dubuisson. 2001. Biogenesis of hepatitis C virus envelope glycoproteins. J. Gen. Virol. 82: 2589-2595 
  17. Reed, K. E. and C. M. Rice. 2002. Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties. Curr. Top. Microbiol. Immunol. 242: 55-84 
  18. Han, B. K., B. Y. Lee, M. K. Min, and K. H. Jung. 1998. Expression and characterization of recombinant E2 protein of hepatitis C virus by insect cellibaculovirus expression system. J. Microbiol. Biotechnol. 8: 361-368 
  19. Baumert, T. F., J. Vergalla, J. Satoi, M. Thomson, M. Lechmann, D. Herion, H. B. Greenberg, S. Ito, and T. J. Lian. 1999. Hepatitis C virus-like particles synthesized in insect cells as a potential vaccine candidate. Gastroenterology 117: 1397-1407 
  20. Grakoui, A., C. Wychowski, C. Lin, S. M. Feinstone, and C. M. Rice. 1993. Expression and identification of hepatitis C virus polyprotein cleavage products. J. Virol. 67: 1385-1395 

이 논문을 인용한 문헌 (7)

  1. 2006. "" Journal of microbiology and biotechnology, 16(2): 308~311 
  2. 2006. "" Journal of microbiology and biotechnology, 16(9): 1362~1368 
  3. 2006. "" Journal of microbiology and biotechnology, 16(9): 1459~1463 
  4. 2006. "" Journal of microbiology and biotechnology, 16(9): 1468~1471 
  5. 2007. "" Journal of microbiology and biotechnology, 17(12): 2076~2080 
  6. 2007. "" Journal of microbiology and biotechnology, 17(10): 1738~1741 
  7. 2007. "" Journal of microbiology and biotechnology, 17(6): 891~896 

원문보기

원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

이 논문과 연관된 기능

DOI 인용 스타일