Choi, Gug-Seoun
(National Horticulture Research Institute, Rural Development Admission)
,
Kim, Jae-Hyun
(National Horticulture Research Institute, Rural Development Admission)
,
Lee, Dong-Hyuk
(National Horticulture Research Institute, Rural Development Admission)
,
Kim, Jeong-Soo
(National Horticulture Research Institute, Rural Development Admission)
,
Ryu, Ki-Hyun
(Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women's University)
We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, w...
We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, we found a number of viruses from 1,056 samples that we collected. These included Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), Pepper mild mottle virus (PMMoV), Broad bean wilt virus 2 (BBWV2), Tobacco mild green mosaic virus (TMGMV), and Tomato spotted wilt virus (TSWV). Of the samples analyzed, $343(32.5\%)$ were infected with CMV, $209(19.8\%)$ with PepMoV, $141(13.4\%)$ with PMMoV, $12(1.1\%)$ with BBWV2, $40(3.8\%)$ with TMGMV, $5(0.5\%)$ with TSWV, $153(14.5\%)$ with CMV and PepMoV, $54 (5.1\%)$ with CMV and PMMoV, $31(2.9\%)$ with PepMoV and PMMoV, $3(0.3\%)$ with CMV and BBWV2, $1(0.1\%)$ with CMV, PepMoV and BBWV2, $8(0.8\%)$ with CMV, PepMoV and PMMoV, and $30 (2.8\%)$ samples were infected with viruses which were not identified. CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper. TMGMV was only found in the southern part of Korea, while TSWV was isolated in Anyang and Yesan. However, we did not encounter in this survey the Alfalfa mosaic virus (AMV), Potato virus Y (PVY), Tobacco mosaic virus (TMV), and Pepper vein chlorosis virus (PVCV).
We conducted a survey on pepper virus diseases in 31 regions in Korea from November 2001 to December 2004. Using electron microscopy, test plant reaction, rapid immuno-filter paper assay (RIPA), reverse transcription-polymerase chain reaction (RT-PCR) and/or analysis of viral nucleotide sequences, we found a number of viruses from 1,056 samples that we collected. These included Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), Pepper mild mottle virus (PMMoV), Broad bean wilt virus 2 (BBWV2), Tobacco mild green mosaic virus (TMGMV), and Tomato spotted wilt virus (TSWV). Of the samples analyzed, $343(32.5\%)$ were infected with CMV, $209(19.8\%)$ with PepMoV, $141(13.4\%)$ with PMMoV, $12(1.1\%)$ with BBWV2, $40(3.8\%)$ with TMGMV, $5(0.5\%)$ with TSWV, $153(14.5\%)$ with CMV and PepMoV, $54 (5.1\%)$ with CMV and PMMoV, $31(2.9\%)$ with PepMoV and PMMoV, $3(0.3\%)$ with CMV and BBWV2, $1(0.1\%)$ with CMV, PepMoV and BBWV2, $8(0.8\%)$ with CMV, PepMoV and PMMoV, and $30 (2.8\%)$ samples were infected with viruses which were not identified. CMV was the most predominant virus in all inspected fields and the number of the samples infected with PMMoV was relatively low as compared PepMoV infection level in pepper. TMGMV was only found in the southern part of Korea, while TSWV was isolated in Anyang and Yesan. However, we did not encounter in this survey the Alfalfa mosaic virus (AMV), Potato virus Y (PVY), Tobacco mosaic virus (TMV), and Pepper vein chlorosis virus (PVCV).
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제안 방법
Kinds of viruses infecting pepper In this study, we have collected 1, 056 pepper samples from 31 areas in Korea, and identified viruses from these samples using DAS-ELTSA, RIPA, RT-PCR, 1C RT-PCR, electron microscopy, and indicator plant assay. About 1, 026 (97.
Frequency of the viruses isolated from pepper in Korea, November 2001 to September 2004. The samples were collected from 31 areas in Korea and were detected by DAS-ELISA, RIPA, RT-PCR, IC/RT-PCR, electron microscopy and/or indicator plant assay.
대상 데이터
Green pepper cultivated using a hydroponic system in a plastic house showed 100% infection at the mid growing stage. The cas니al viruses were two tobamovimses, TMGMV and PMMoV.
성능/효과
In conclusion, we conchide that CMV PepMoY and PMMoV are the most frequent viruses infecting pepper in Korea. TSWV might be able to cause severe damage in the future if researchers do not come up with preventive management practices.
2%) of these samples were identified to have one- or mix-infected viruses. Of the samples analyzed, 343 (32.5%) were infected with CMV 209 (19.8%) with PepMoV 141 (13.4%) with PMMoV, 12 (1.1%) with BBWV2, 40 (3.8%) with TMGMV 5 (0.5%) with TSWY 153 (14.5%) with CMV and PepMoV 54(5.1%) with CMV and PMMoV, 31 (2.9%) with PepMoV and PMMoV, 3 (0.3%) with CMV and BBWV2, 1 (0.1%) with CMY PepMoV and BBWV2, 8 (0.8%) with CMY PepMoV and PMMoV and 30 (2.8%) samples were infected with vimses which were not identified (Fig. 1).
However, we did not detect PVY PVX, TMY ToMV A1MV and ChiVMoV from the samples. The major viruses detected in pepper were CMY PepMoV and PMMoV The sin이e and mixture infection rates of these viruses were 71.1% and 26.1%, respectively. No virus was identified 400 from 30 samples showing viral disease-like symptoms and these symptomes were probably caused by pesticide damage, physiological disorder, or unidentified virus.
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