The copper-containing enzyme, tyrosinase, catalyzes the oxidation of tyrosine into dihydroxy phenylalanine (DOPA) and subsequently DOPAquinone. It is responsible, not only for the pigment melanin biosynthesis in human skin, but also for browning in foods. In the present study, tyrosinase inhibitory ...
The copper-containing enzyme, tyrosinase, catalyzes the oxidation of tyrosine into dihydroxy phenylalanine (DOPA) and subsequently DOPAquinone. It is responsible, not only for the pigment melanin biosynthesis in human skin, but also for browning in foods. In the present study, tyrosinase inhibitory and antioxidant activities of Korean mistletoe extract and its fractions were investigated. As a result, both water and methanol (MeOn) extracts inhibited the tyrosinase activity. Among the fractions, the fraction eluted with $95\%$ MeOn significantly inhibited the tyrosinase activity. The fraction was further purified, and the purified fraction C strongly inhibited the enzyme activity up to $92\%$. In addition, water and methanol extracts exerted radical scavenging effects. The fractions eluted with $70\%\;MeOn\;and\;95\%$ Me on showed high radical scavenging activities. In conclusion, these results suggest that Korean mistletoe extract and its fractions might be useful for the treatment of various dermatological disorders such as epidermal hyperpigmentation and for improving food quality.
The copper-containing enzyme, tyrosinase, catalyzes the oxidation of tyrosine into dihydroxy phenylalanine (DOPA) and subsequently DOPAquinone. It is responsible, not only for the pigment melanin biosynthesis in human skin, but also for browning in foods. In the present study, tyrosinase inhibitory and antioxidant activities of Korean mistletoe extract and its fractions were investigated. As a result, both water and methanol (MeOn) extracts inhibited the tyrosinase activity. Among the fractions, the fraction eluted with $95\%$ MeOn significantly inhibited the tyrosinase activity. The fraction was further purified, and the purified fraction C strongly inhibited the enzyme activity up to $92\%$. In addition, water and methanol extracts exerted radical scavenging effects. The fractions eluted with $70\%\;MeOn\;and\;95\%$ Me on showed high radical scavenging activities. In conclusion, these results suggest that Korean mistletoe extract and its fractions might be useful for the treatment of various dermatological disorders such as epidermal hyperpigmentation and for improving food quality.
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가설 설정
Recently we reported the inhibition of porcine pancreatic elastase by mistletoe extract (24). In this study, we investigated the tyrosinase inhibitory and antioxidant activity of mistletoe extract and its fractions.
제안 방법
The tyrosinase activity, using『Dopa as a substrate, was measured according to the method of Kubo and Kinst-Hori (8) with slight modifications. The mushroom tyrosinase (EC 1.
데이터처리
All laboratory experiments were performed in triplicates, Data points and error bars on the figures represent mean ± standard error of the mean (SEM) for each experiment.
성능/효과
To study the effects on tyrosinase inhibition by fraction A, B and C, we first assumed from the previous experiments that 30 min is the optimal time to incubate the enzyme-reacted solutions. As a result, compared with those of 30% and 95% MeOH fraction, the fraction C at 3 mg/mL significantly inhibited the enzyme activity up to 92% while the inhibitory effects of fraction A and B were moderately increased (Fig. 6).
In conclusion, these results suggest that Korean mistletoe extract may be used for the treatment of pathological processes such as various dermatological disorders and improving food quality. The isolation and the structural elucidation of the active constituents of the extracts will provide useful leads in the development of skin-whitening agents.
On the other hand, water and 70% MeOH fractions showed inhibitory effects at lower doses but these effects gradually declined at higher doses. On the half-inhibition concentration (IC5o) for the fractions, the 95% MeOH fraction had the lowest IC50 value (1.3 mg/mL) indicating that it has the highest tyrosinase inhibitory effect, while the 70% MeOH fraction showed the lowest effect (Table 1). At higher concentrations of the 95% MeOH fraction, up to 5 mg/mL, the fraction inhibited the tyrosinase activity by 83%.
후속연구
The isolation and the structural elucidation of the active constituents of the extracts will provide useful leads in the development of skin-whitening agents. Therefore, there is a need for further research focusing on isolating and identifying the effective tyrosinase inhibitory components in mistletoe and its inhibitory effect on melanin synthesis.
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