[논문]New Evidences of Effect of Melanocortin-4 Receptor and Insulin-like Growth Factor 2 Genes on Fat Deposition and Carcass Traits in Different Pig Populations

Chen, J.F.; Xiong, Y.Z.; Zuo, B.; Zheng, R.; Li, F.E.; Lei, M.G.; Li, J.L.; Deng, C.Y.; Jiang, S.W.

doi:10.5713/ajas.2005.1542

New Evidences of Effect of Melanocortin-4 Receptor and Insulin-like Growth Factor 2 Genes on Fat Deposition and Carcass Traits in Different Pig Populations 원문보기

Asian-Australasian journal of animal sciences, v.18 no.11, 2005년, pp.1542 - 1547

Chen, J.F. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Xiong, Y.Z. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Zuo, B. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Zheng, R. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Li, F.E. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Lei, M.G. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Li, J.L. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Deng, C.Y. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University) , Jiang, S.W. (Agricultural Ministry Key Laboratory of Swine Breeding and Genetics, Huazhong Agricultural University)

Abstract ▼ AI-Helper

The Melanocortin-4 Receptor (MC4R) and Insulin-like Growth Factor 2 (IGF2) are two important candidate genes related to fat deposition and carcass traits. MC4R was found on study on human obesity and then was studied as candidate gene affecting food intake and fat deposition traits in mice and pigs. Insulin-like Growth Factor 2 (IGF2) gene plays an important role on tumor cell proliferation and muscle growth. It also affects fat traits and live weight in pigs. In this paper, MC4R and IGF2 were studied as two candidate genes associated with important economic traits such as fat deposition and carcass traits in five different pig populations. Taq I-PCR-RFLP and Bcn I-PCR-RFLP were respectively used to detect the polymorphism of genotypes of MC4R and IGF2 genes. Different MC4R genotype frequencies were observed in four populations. IGF2 genotype frequencies were also different in two populations. The results of association analysis show both MC4R and IGF2 genes were significantly associated with fat deposition and carcass traits in about 300 pigs. This work will add new evidence of MC4R and IGF2 affecting fat deposition and carcass traits in pigs and show that two genes can be used as important candidate genes for marker assistant selection (MAS) of growth and lean meat percentage in pigs.

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제안 방법

Based on these two variances G→C, we designed two pairs of primers and used Bcn I-PCR-RFLP to analyze the association of the variances with fat and carcass traits in a total of 311 pigs belonging to different populations.
Based on these two variances GtC, we designed two pairs of primers and used Bcn I-PCR-RFLP to analyze the association of the variances with fat and carcass traits in a total of 311 pigs belonging to different populations.
The fat deposition and carcass traits were measured and calculated. To investigate the effects of these mutations, some populations of which genetic background is close were pooled for an across-population (population C and population E) analysis.
Those genes including MC4R and IGF2 affecting animal obesity could be candidate genes associated with fat thickness and carcass character of pigs. The result of this study could add new important evidences that MC4R, IGF2 are two important candidate genes to be used for selecting of fat deposition and lean meat percentage in pig industry.
The results reported in this work provide new important evidence in favor of the mutations we have detected in MC4R and IGF2 genes affecting fat deposition and carcass traits in pigs. This conclusion is based on two points: (1) the mutations detected in five different populations in total of about 300 pigs were all observed polymorphisms and associated with five fat deposits and four carcasses traits;(2) Aspartic acid found at position 298 of the seventh transmembrane domain is very highly conserved in the MCR proteins, and the mutation in highly conserved regions may cause structural changes and alter the function of the receptor (Kim et al.
, 2000b). This study will use Taq I-PCR-RFLP to analyze the association of this variant with fat deposition and carcass traits in a total of 275 pigs belonging to different populations.

대상 데이터

For IGF2 gene, primer 1 (F: 5' CCT TCG GGA TGG ATG GTG 3', R: 5' GCC TTT ATT CTT ATT GGT TTT CAA C 3') and primer 2 (F: 5' AAG CGC CTT TCA CTT CTG 3', R: 5' TCT GAA GTC TCA GAC CCT CG 3') were designed from pig IGF2 DNA sequence (GenBank access number AY044828).

성능/효과

For IGF2, although there is no polymorphism in population A, B and C, significant difference and highly significant difference on TFT and DP was respectively observed in Chinese breed (Population D). The highly significant difference on DP was also observed in population E.
For MC4R, in each population, frequency of allele A is much lower than that of allele B, and allele A frequency is zero in MS (population D), a Chinese pig breed especially. For IGF2, population A, B and C are all DD genotype, however, frequency of allele D is lower than that of allele C in MS (population D) and the crossbreeding populations between LW and MS (LWxMS and MSxLW) (population E).
In this paper, two candidate genes MC4R and IGF2 can explain significant differences for backfat, internal fat, fat meat percentage, lean meat percentage and dress percentage in five populations of pigs. These results illustrate the potential value of Marked Assistance Selection (MAS) with candidate genes in livestock industry.
Significant (p<0.05) differences of FMP, IFP and highly significant (p<0.01) difference LMP were only observed in population E, in which lower LS means of FMP, IFP and higher LMP on genotype BB were observed.
05). Significant (p<0.05) differences of FMP, IFP and highly significant (p<0.01) difference LMP were only observed in population E, in which lower LS means of FMP, IFP and higher LMP on genotype BB were observed. Higher LS means of DP on genotype BB was also observed in population E, though it is not significant (See Table 3).
The results reported in this work provide new important evidence in favor of the mutations we have detected in MC4R and IGF2 genes affecting fat deposition and carcass traits in pigs. This conclusion is based on two points: (1) the mutations detected in five different populations in total of about 300 pigs were all observed polymorphisms and associated with five fat deposits and four carcasses traits;(2) Aspartic acid found at position 298 of the seventh transmembrane domain is very highly conserved in the MCR proteins, and the mutation in highly conserved regions may cause structural changes and alter the function of the receptor (Kim et al., 2000b).

참고문헌 (26)

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상세보기
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