Zeng, Y.Q.
(College of Animal Science and Technology, Shandong Agricultural University)
,
Wang, G.L.
(College of Animal Science and Technology, Nanjing Agricultural University)
,
Wang, C.F.
(College of Animal Science and Technology, Shandong Agricultural University)
,
Wei, S.D.
(Station of Popularization of Animal Science and Veterinary Technology of Laiwu)
,
Wu, Y.
(Institute of Animal Science, Shandong Academy of Agricultural Sciences)
,
Wang, L.Y.
(College of Animal Science and Technology, Nanjing Agricultural University)
,
Wang, H.
(College of Animal Science and Technology, Shandong Agricultural University)
,
Yang, H.L.
(College of Animal Science and Technology, Shandong Agricultural University)
This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The ...
This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic HinfI site in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as $HinfI^*$) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of $HinfI^*$ in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and $HinfI^*$ HFABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.
This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCRRFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic HinfI site in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as $HinfI^*$) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of $HinfI^*$ in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and $HinfI^*$ HFABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.
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문제 정의
, 1989), might provide additional insight into H-FABP gene's role in this trait. Therefore, the aim of this study was to detect genetic variants of H-FABP gene in the Laiwu Black and four western pig breeds and to estimate the association of genetic variants of H-FABP gene with IMF deposition.
, 1989), might provide additional insight into H-FABP gene's role in this trait. Therefore, the aim of this study was to detect genetic variants of H-FABP gene in the Laiwu Black and four western pig breeds and to estimate the association of genetic variants of H-FABP gene with IMF deposition.
대상 데이터
223 pigs including one Chinese native breed (Laiwu Black-LB) and four western breeds (Landrace-LA, Large Yorkshire-LY, Hampshire-HA, Duroc-DU) were involved in this study. Experimental pigs were housed in groups and fed ad libitum at three breeding farms until the slaughter weight of 90-100 kg.
223 pigs including one Chinese native breed (Laiwu Black-LB) and four western breeds (Landrace-LA, Large Yorkshire-LY, Hampshire-HA, Duroc-DU) were involved in this study. Experimental pigs were housed in groups and fed ad libitum at three breeding farms until the slaughter weight of 90-100 kg. At slaughter, ear samples were collected for genomic DNA extraction and muscle samples of the longissimus dorsi at the third lumbar vertebra were obtained for meat quality evaluation.
이론/모형
is the random residual effect. Statistical analysis was performed with SAS (1990) by use of the GLM procedure. Duncans Multiple Range Test was performed to separate means of significant difference.
성능/효과
For HaeIII and HinfI* H-FABP RFLPs, significant (p<0.05) contrasts of -0.69% (2.98-3.67%) and 0.72% (3.55-2.83%) were detected in the least square means of IMF content between the homozygous genotype DD and dd, BB and bb classes, respectively.
78%) was observed in the least square means of IMF content between the homozygous genotype HH and hh classes. For HaeIII and HinfI* H-FABP RFLPs, significant (p<0.05) contrasts of -0.69% (2.98-3.67%) and 0.72% (3.55-2.83%) were detected in the least square means of IMF content between the homozygous genotype DD and dd, BB and bb classes, respectively. Obviously, for H-FABP RFLP the HHddBB genotype had the highest IMF content in this experimental population.
Results (Table 3) of the least square means (LSM) of IMF content for different genotypes indicated that genotype classes differed significantly (p<0.05).
Results (Table 3) of the least square means (LSM) of IMF content for different genotypes indicated that genotype classes differed significantly (p<0.05). The contrast between the homozygous genotype classes, IMF (XX)- IMF(xx), was estimated, where XX and xx are the homozygous genotype class for each H-FABP RFLP.
후속연구
The evidence that the H-FABP gene is responsible for part of the genetic variation in IMF content in pigs reveals the possibility to improve meat quality of pigs by markerassisted selection according to polymorphisms in the HFABP gene. However, the role of H-FABP in IMF deposit and associations in other pig breeds that have a polymorphic H-FABP gene need to be investigated further. Moreover, it is of interest to study genetic variants of the HFABP gene and its association with IMF content on a larger scale of pig population.
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