• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보


We performed in vitro and in vivo studies to know whether the inhibitory effects of ginsenosides on $5-HT_{3A}$ receptor channel acctivity are coupled to anti-nausea and anti-vomiting action. In vitro study, we investigated the effect of compound K (CK) and M4, which are ginsenoside metabolites, on human $5-HT_{3A}$ receptor channel activity expressed in Xenopus oocytes using two-electrode voltage clamp technique. Treatment of CK or M4 themselves had no effect in both oocytes injected with $H_2O\;and\;5-HT_{3A}$ receptor cRNA. In oocytes injected with $5- HT_{3A}$ receptor cRNA, M4 treatment inhibited more potently 5-HT-induced inward peak current $(I_{5-HT})$ than CK with dose-dependent and reversible manner. The half-inhibitory concentrations $(IC_{50})$ of CK and M4 were $36.9\;\pm\;10.1\;and\;7.3\;\pm\;2.2\;{\mu}M$, respectively. The inhibition of $I_{5-HT}$ by M4 was non-competitive and voltage-independent. These results indicate that M4 might regulate $5-HT_{3A}$ receptors. In vivo experiments, injection of cisplatin (7.5 mg/kg, i.v.) induced both nausea and vomiting with 1 h latency. These episodes reached to peak after 2 h and persisted for 4 h. Pre-treatment of GTS (500 mg/kg, p.o.) significantly reduced cisplatin-induced nausea and vomiting by $51\;\pm\;8.4\;and\;48.8\;\pm\;6.4\%$ during 4 h compared to GIS­untreated group, respectively. These results show the possibility that in vitro inhibition of $5-HT_{3A}$ receptor channel activity by ginsenosides might be coupled to in vivo anti-emetic activity.

참고문헌 (23)

  1. Abe, T. : Clinical studies of the vitamins. Jpn. Soc. Inter. Med. 54, 989-1006 (1965) 
  2. Attele, A. S., Wu, J. A. and Yuan, C. S. : Ginseng pharmacology: multiple onstituents and multiple actions. Biochem. Pharmacol. 58, 1685-1693 (1999) 
  3. Kudo, K., Tachikawa, E., Kashimoto, T. and Takahashi, E. : Properties of ginseng saponin inhibition of catecholamine secretion in bovine chromaffin cells. Eur. J. Pharmacol. 341, 139-144 (1998) 
  4. Choi, S., lung, S. Y., Lee, J. H., Sala, F., Criado, M., Mulet, J., Valor, L. M., Sala, S., Engel, A. G. and Nah, S. Y. : Effects of ginsenosides, active components of ginseng, on nicotinic acetylcholine receptors expressed in Xenopus oocytes. Eur. J. Pharmacol. 442, 37-45 (2002) 
  5. Sala, F., Mulet, J., Choi, S., lung, S. Y., Nah, S. Y., Rhim, H., Valor, L. M., Criado, M. and Sala, S. : Effects of ginsenoside $Rg_2$ on human neuronal nicotinic acetylcholine receptors. J. Pharmacol. Exp. Ther. 301, 1052-1059 (2002) 
  6. Karikura, M., Miyase, T, Tanizawa, H., Taniyawa, T. and Takino, Y. : Studies on absorption, distribution, excretion and metabolism of ginseng saponins. VII. Comparison of the decomposition modes of ginsenoside $Rb_1$ and $Rb_2$ in the digestive tract of rats. Chem. Pharm. Bull. 39, 400-404 (1991) 
  7. Wakabayashi, C., Hasegawa, H., Murata, J. and Saiki, I. : In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oneol. Res. 9, 411-417 (1997) 
  8. Wakabayashi, C., Murakami, K, Hasegawa, H., Murata, J. and Saiki, I. : An intestinal bacterial metabolite of ginseng protopanaxadiol saponins has the ability to induce apoptosis in tumor cells. Biochem. Biophys. Res. Commun. 249, 725-730 (1998) 
  9. Ortells, M. O. and Lunt, G G : Evolutionary history of the ligand-gated on channel superfamily of receptors. Trends Neurosci. 18, 121-127 (1995) 
  10. Jackson, M. B. and Yakel, J. L. : The 5-$HT_3$ receptor channel. Annu. Rev. Physiol. 57, 447-468 (1995) 
  11. Lucot, J. B. : Blockade of 5-$hydroytryptamine_3$ receptors prevents cisplatin-induced but not motion- or xylazine-induced emesis in the cat. Pharmacol. Biochem. Behav. 32, 207-210 (1989) 
  12. Schworer, H., Racke, K. and Kilbinger, H. : Cisplatin increases the release of 5-hydroxytryptamine (5-HT) from the isolated vascularly perfused small intestine of the guineapig-involvement of 5-$HT_3$ receptors. Naunyn-Schmiedeberg's Arch. Pharmacol. 344, 143-149 (1991) 
  13. King, G. L.: Animal models in the study of vomiting. Can. J. Physiol. Pharmacol. 68,260-268 (1990) 
  14. Dang, H., England, P. M., Farivar, S. S., Dougherty, D. A. and Lester, H. A. : Probing the role of a conserved Ml proline residue in 5$hydroytryptamine_3$receptor gating. Mol. Pharmacol. 57, 1114-1122 (2000) 
  15. Arias, H. R. : Luminal and non-luminal non-competitive inhibitor binding sites on the nicotinic acetylcholine receptor. Mol. Membr. Biol. 13, 1-17 (1996) 
  16. Heidman, T, Oswald, R. E. and Changeux, J. P. : Multiple sites of action for noncompetitive blockers on acetylcholine receptor rich membrane fragments from Torpedo marmorata. Biochemistry 22, 3112-3127 (1983) 
  17. Sine, S. M. and Taylor, P. : Local anesthetic and histrionicotoxin are allosteric inhibitors of the acetylcholine receptor. J. Biol. Chem. 257, 8106-8114 (1982) 
  18. Fortney, J. T, Gan, T. J., Graczyk, S., Wetchler, B., Melson, T., Kahlil, S., Mckenzie, R., Pamillo, S., Glass, P. S., Moote, C., Wermeling, D., Parasuraman, T. V., Duncan, B. and Creed, M. R. : A comparison of the efficacy, safety, and patient satisfaction of ondansetron versus droperidol as antiemetics for elective outpatient surgical procedures. S3A-409 and S3A-410 Study Groups. Anesth. Analg. 86, 731-738 (1998) 
  19. Perez, E. A. and Gandara, D. R. : Advances in the control of chemotherapy-induced emesis. Ann. Oncol. 3 Suppl. 3, 47-50 (1992) 
  20. Polati, E., Verlato G., Finco, G., Monsaner, W., Grosso, S., Gottin, L., Pinaroli, A. M. and Ischia, S. : Ondansetron versus metoclopramide in the treatment of postoperative nausea and vomiting. Anesth. Analg. 85, 395-399 (1997) 
  21. Chevallier, B. : The control of acute cisplatin-induced emesis a comparative study of granisetron and a combination regimen of high-dose metoclopramide and dexamethasone. Br. J. Cancer 68, 176-180 (1993) 
  22. Lee, B.-H., Lee, J.-H., Jung, S.-M., Kim, D.-H., Kim, J.-H., Kim, J.-I., Lee, S.-M. and Na, S.-Y. : Differential Effect of Ginsenoside Metabolites on the 5-$HT_3A$ Receptor-Mediated Ion Current in Xenopus Oocytes. Mol. Cells, 17, 1, 51-56 (2004) 
  23. Kanaoka, M., Akao, T. and Kobashi, K. : Metabolism of ginseng saponins, ginsenosides by human intestinal flora. J. Trad. Med. 11, 241-245 (1994) 

이 논문을 인용한 문헌 (0)

  1. 이 논문을 인용한 문헌 없음

DOI 인용 스타일