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미생물 Chitin Deacetylase의 특성과 응용
Enzymatic Characteristics and Applications of Microbial Chitin Deacetylases 원문보기

한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, v.33 no.1, 2005년, pp.9 - 15  

국주희 (전남대학교 응용생물공학부, 농업과학기술연구소) ,  정우진 (전남대학교 응용생물공학부, 농업과학기술연구소) ,  김길용 (전남대학교 응용생물공학부, 농업과학기술연구소) ,  박노동 (전남대학교 응용생물공학부, 농업과학기술연구소)

초록
AI-Helper 아이콘AI-Helper

Chitin deacetylase(CDA; EC 3.5.1.41)는 키틴의 N-acetamide bonds를 가수분해하여 이를 키토산으로 전환시키는 효소다. 한편, 키토산은 의약, 화장품, 식품, 농업 등의 분야에서 다양하게 응용되는 고분자 다당류이다. 본 논문에서는 미생물 유래 CDA의 분포, 분석법, 효소적 특성, 기질 특이성, 작용기작, 유전자의 구조, 생물학적 역할, 응용 등의 최신 지견을 기술하고자 하였다. 미생물 CDA가 세포벽 형성과 식물-미생물 상호작용에 관여한다는 연구결과들을 제시하였으며, CDA의 유전자 구조를 다양한 acetylated poly/oligo-saccharides를 탈아세틸화하는 family 4 carbohydrate esterase의 유전자 구조와 비교하였다. 키틴의 탈아세틸화로 키토산을 제조하는 과정에 CDA의 활용 가능성과, CDA를 포함한 고활성의 키틴 대사효소들을 분비하는 곤충 병원균의 활용 가능성도 살펴보았다.

Abstract ▼ AI-Helper 아이콘AI-Helper

Chitin deacetylase (CDA; EC 3.5.1.41) catalyzes the hydrolysis of N-acetamide bonds of chitin, converting it to chitosan. Chitosan has several applications in areas such as biomedicine, food ingredients, cosmetics, pharmaceuticals, and agriculture. In this paper, occurrence, assay and purification p...

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