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NTIS 바로가기생명과학회지 = Journal of life science, v.18 no.11 = no.103, 2008년, pp.1617 - 1624
Chitin and chitosan, which is deacetylated form of chitin, are one of the most abundant biomass on the earth. They showed various biological activities including antimicrobial activity, heavy metal chelating, immune system activation, and have very diverse applications in food, pharmaceutical, medic...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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키틴분해효소가 기질에 작용하는 메커니즘 중 retaining 방식이란 무엇인가? | 앞서 말한 대로 키틴분해효소가 기질에 작용하는 메커니즘을 크게 두가지로 나눌 수 있는데 GH18 키틴분해효소는 retaining, GH19 키틴분해효소는 inverting 메커니즘[3,11]을 통해 당쇄 결합을 분해하는 것으로 밝혀졌다. Retaining과 inverting 방식 모두 두 개의 음이온성 아미노산이 반응에 관여하는데 retaining 방식은 하나의 음이온성 아미노산이 친핵체(nucleophile)로 작용하고 다른 하나의 음이온성 아미노산(주로 Glu)이 산/염기로 작용하여 가수 분해 후 anomeric form이 유지되는 방식이다. 반대로 inverting 방식은 두 개의 음이온성 아미노산이 모두 산/염기로 작용하여 가수분해 후 anomeric form이 변하는 방식이다(Table 1). | |
키토산의 어떤 생리 기능성을 가지고 있는가? | 키틴은 매년 해양 무척추동물, 곤충, 곰팡이 등에 의해 1 × 109톤 정도가 생합성되고 있으며 곰팡이의 세포벽, 절족류, 강장동물, 연체동물 및 선충류 등 하등동물 껍질, 균류와 조류 등 하등식물의 세포벽에 존재한다. 반면 자연계중에 일부 곰팡이의 세포벽[70]에 존재하며 키틴의 탈아세틸화를 통해 만들어지는 키토산(chitosan)은 항균활성[1] , 중금속 흡착능력[6], 면역증강효과[15], 바이오필름형성[5] 등 다양한 생리 기능성 때문에 의약품 원료 및 기능성 식품 등으로 많은 주목을 받아왔다. | |
키틴이란? | 키틴(chitin)은 β-D-glucose의 C-2 hydroxyl기가 acetylamino기로 치환된 N-acetyl-D-glucosamine (GlcNAc)이 (β1→4) 결합에 의해 구성된 고분자 화합물로서 지구상에 셀룰로스 다음으로 가장 많이 생산되는 바이오매스이다. 키틴은 매년 해양 무척추동물, 곤충, 곰팡이 등에 의해 1 × 109톤 정도가 생합성되고 있으며 곰팡이의 세포벽, 절족류, 강장동물, 연체동물 및 선충류 등 하등동물 껍질, 균류와 조류 등 하등식물의 세포벽에 존재한다. |
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