[논문]Improvement of <tex>$\\gamma-Aminobutyric$</tex> Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057

Choi Soo-Im; Lee Jae-Won; Park Sang-Min; Lee Moo-Young; Ji Geun-Eog; Park Myeong-Soo; Heo Tae-Ryeon

Improvement of $\\gamma-Aminobutyric$ Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057 원문보기

Journal of microbiology and biotechnology, v.16 no.4, 2006년, pp.562 - 568

Choi Soo-Im (Department of Biological Engineering, Inha University) , Lee Jae-Won (Department of Biological Engineering, Inha University) , Park Sang-Min (Department of Biological Engineering, Inha University) , Lee Moo-Young (Department of Food and Nutrition, Seoul National University) , Ji Geun-Eog (Department of Food and Nutrition, Seoul National University, Research Center, BIFIDO Co., Ltd.) , Park Myeong-Soo (Research Center, BIFIDO Co., Ltd., Department of Nutrition and Hotel Culinary Art, Anyang Technical College) , Heo Tae-Ryeon (Department of Biological Engineering, Inha University)

Abstract ▼ AI-Helper

GABA $(\gamma-aminobutyric\;acid)$ is the principal inhibitory neurotransmitter in the brain. For the efficient production of GAB A, a semi continuous cell entrapment system using Lactobacillus brevis GABA 057 was optimized, including the substrate concentration, bead-stabilizing additives, and reaction conditions. The converted monosodium glutamate (MSG), which was added as a substrate for glutamic acid decarboxylase (GAD), increased from 2% (w/v) to 12% (w/v). The addition of isomaltooligosaccharide to the alginate beads also increased the stability of the entrapped L. brevis and GABA productivity. Consequently, when optimal conditions were applied, up to 223 mM GABA could be produced from 534 mM MSG after 48 h of reaction by using alginate-beadencapsulated L. brevis GABA 057.

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가설 설정

brevis GABA 057. A. Effect of media additives on GABA production. B.

제안 방법

A qualitative analysis of GABA was performed using TLC with a cellulose F aluminum plate (Merck Co., Germany) in a solvent mixture of n-butanol-acetic acid-water (4:1:1).
), after PITC (phenylisothiocyanate) derivatization. GABA analysis was performed using a Hewlett Packard 1100 series HPLC with a Waters Symmetry C₁₈ column (5 μ, 4.6><250 mm I.D.), HP 1100 series binary pump, HP 1100 series autosampler, column oven (46℃), and HP 1100 series UV detector (254 nm). The elution solvent system was comprised of (A) 1.
In this study, we have established a set of optimal conditions for the mass production of GABA by Lactobacillus brevis GABA 057 as a GABA-prod니cing organism, using a sodium alginate entrapment system.
2 at 12-h intervals (—△) throughout the culture period. One batch culture period was 48 h, and culture medium samples for analyzing the GABA conversion rate were collected at 12-h intervals, and the viable cell count was then assessed as described in Materials and Methods.

성능/효과

brevis GABA 057 that was entrapped in alginate and isomaltooligosaccharide, and also determined the optimal conditions for this process. The results showed that 12% MSG (640.8 mM) was completely converted into GABA at 34℃ and pH 4.2 within a 48-h culture period. Furthermore, the LAB entrapment system made it possible to reuse the beads up to 4 times in a batch fermentation system, because of increase of stability and viability, thereby maximizing the GABA productivity.

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Improvement of $\\gamma-Aminobutyric$ Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057 원문보기

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Improvement of $\\gamma-Aminobutyric$ Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057 원문보기

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