[논문]The Effects of Stress Related Genes on Carcass Traits and Meat Quality in Pigs

Jin, H.J.; Park, B.Y.; Park, J.C.; Hwang, I.H.; Lee, S.S.; Yeon, S.H.; Kim, C.D.; Cho, C.Y.; Kim, Y.K.; Min, K.S.; Feng, S.T.; Li, Z.D.; Park, C.K.; Kim, C.I.

doi:10.5713/ajas.2006.280

[국내논문] The Effects of Stress Related Genes on Carcass Traits and Meat Quality in Pigs 원문보기

Asian-Australasian journal of animal sciences, v.19 no.2, 2006년, pp.280 - 285

Jin, H.J. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) , Park, B.Y. (NLRI, RDA) , Park, J.C. (NLRI, RDA) , Hwang, I.H. (Department of Animal Resources and Biotechnology, Chonbuk National University) , Lee, S.S. (National Institute of Subtropical Agriculture, RDA) , Yeon, S.H. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) , Kim, C.D. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) , Cho, C.Y. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) , Kim, Y.K. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) , Min, K.S. (Graduate School of Bio. & Information Technology, Hankyong National University) , Feng, S.T. (Institute of Animal Science) , Li, Z.D. (College of Biological Science, China Agricultural University) , Park, C.K. (College of Animal Resource Science, Kangwon National University) , Kim, C.I. (College of Animal Resource Science, Kangwon National University)

Abstract ▼ AI-Helper

The current study was conducted to investigate the relationship between stress related gene and meat quality in pigs. A total number of 212 three-way cross bred (Landrace-$Yorkshire{\times}Duroc$) and 38 Duroc were sampled from the Korean pig industry to determine genotype frequency of porcine stress syndrome (PSS) and heat shock protein 70 kDa (HSP70) genes and their relationship with carcass traits and longissimus meat quality. Screen of HSP70 was performed by the single strand conformation polymorphism (SSCP) technique. Based on the analysis of restriction fragment length polymorphism (RFLP) in ryanodine receptor 1 (RYR1) gene, genetic disorder of PSS was related to a mutation at $18,168^{th}$ (C to T) of exon 17. There was no significant difference in ultimate meat pH and backfat thickness between HSP70 K1-AA type and -BB type in pure Duroc breed. In Landrace-$Yorkshire{\times}Duroc$ (L-$Y{\times}D$) cross bred pig, our results indicated that HSP70 derivate type in Duroc had a limited effect on backfat thickness, but L-$Y{\times}D$ type had a noticeable linkage with HSP70 K1-AA and K3-AB. This tendency was also observed in hot carcass weight where HSP70 K1-AA and K3-AB resulted in heavier weight with 86.3 kg compared to HSP70 K1-AB and K3-BB of 74.3 kg. Results imply that stress related HSP70 genotype has a potential association with backfat thickness and carcass weight.

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문제 정의

, 1993; Rosenvold and Andersen, 2003). Therefore, the purpose of this study was to determine the effects of RYR1 and HSP70 genes on carcass characteristics in pigs.

대상 데이터

Number of animals and genetic characteristics were described in Tables 1 and 2. M. longissimus samples were taken from two hundred and twelve pigs from commercial abattoir (Han Naeng Co. LTD., Ochang, Chungbuk) in Korea, while 38 blood samples were collected from the National Livestock Research Institute (NLRI). Genomic DNA was isolated from both whole blood and muscle samples, and purified according to the method of Sambrook et al.
The effects of PSS and HSP70 genotypes on objective meat qualities were evaluated by analysis of variance using GLM procedure of SAS/STAT. The model included HSP70 K1 genotype effect for pure Duroc Breed and PSS-HSP70 K1-HSP70 K3 for three-way cross bred. Differences in least squares means were analysed by Fisher’s least significant difference test (SAS, 1990) with a comparison error rate of 0.

데이터처리

The model included HSP70 K1 genotype effect for pure Duroc Breed and PSS-HSP70 K1-HSP70 K3 for three-way cross bred. Differences in least squares means were analysed by Fisher’s least significant difference test (SAS, 1990) with a comparison error rate of 0.05.
The effects of PSS and HSP70 genotypes on objective meat qualities were evaluated by analysis of variance using GLM procedure of SAS/STAT. The model included HSP70 K1 genotype effect for pure Duroc Breed and PSS-HSP70 K1-HSP70 K3 for three-way cross bred.

성능/효과

Our results indicate that HSP70 genotype from Duroc had a limited effect on meat quality characteristics but LY×D crossbred had a noticeable linkage with HSP70 K1- AA and K3-AB. This effect was observed in the hot carcass weight where significantly (p<0.
91 kg). The PSS-Normal genotype HSP70 K1-BB and K3-AB had the highest yield of tenderloin (442.9 g) while the lowest tenderloin yield was produced by the PSS-Normal genotype HSP70 K1-AB and K3-AB (434.5 g). Leach et al.

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