[논문]Regions in China identification and quality control of radix Codonopsis by chemical fingerprint: Evaluation of lobetyolin from different cultivated

Chou, Gui X; Gao, Qiu T; Li, Jun; Duan, Ran; Cheung, Anna WH; Chu, Glanice KY; Jiang, Zhi Y; Dong, Tina TX; Tsim, Karl WK

doi:10.3742/opem.2006.6.4.293

Regions in China identification and quality control of radix Codonopsis by chemical fingerprint: Evaluation of lobetyolin from different cultivated 원문보기

Oriental pharmacy and experimental medicine, v.6 no.4, 2006년, pp.293 - 299

Chou, Gui X (Department of Biology, Hong Kong University of Science and Technology) , Gao, Qiu T (Department of Biology, Hong Kong University of Science and Technology) , Li, Jun (Department of Biology, Hong Kong University of Science and Technology) , Duan, Ran (Department of Biology, Hong Kong University of Science and Technology) , Cheung, Anna WH (Department of Biology, Hong Kong University of Science and Technology) , Chu, Glanice KY (Department of Biology, Hong Kong University of Science and Technology) , Jiang, Zhi Y (Department of Biology, Hong Kong University of Science and Technology) , Dong, Tina TX (Department of Biology, Hong Kong University of Science and Technology) , Tsim, Karl WK (Department of Biology, Hong Kong University of Science and Technology)

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By using high-performance liquid chromatography-photodiode array detection, a simple and accurate chromatographic fingerprint method was developed for the identification of Radix Codonopsis (roots of Codonopsis) from different sources. Eighteen herbs of Codonopsis at different habitats in China, including roots from Codonopsis pilosula, Codonopsis pilosula var. modesta and Codonopsis tangshen were analyzed by the fingerprint. The amount of lobetyolin was calibrated, which was found to be more consistent in roots of Codonopsis pilosula as compared to that of Codonopsis pilosula var. modesta and Codonopsis tangshen. Having the fingerprint results, hierarchical clustering analyses were performed to classify the eighteen herbs into three groups: Codonopsis pilosula, Codonopsis pilosula var. modesta and Codonopsis tangshen. This clustering analysis agrees very well with the pharmacognostic identification result, and which could be used as a tool in the quality control of Radix Codonopsis.

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to KWKT. This work is part of a project on the study of some common Chinese Material Medica in Hong Kong being undertaken by Department of Health, Hong Kong SAR Government, China.

제안 방법

corresponding HPLC fingerprints. By using the results from HPLC analyses, different samples of Codonopsis roots were subject to hierarchical clustering analysis. The HPLC peaks at absorbance of 267 nm that had good resolution and peak shape, after the normalization, were selected, and therefore 15 constituents including lobetyolin were eluted and calibrated.
In order to achieve the best resolution in HPLC fingerprints as well as the calibration of lobetyolin, the extracting condition of Radix Codonopsis was determined. Methanol extract of Radix Codonopsis showed a poor resolution of peaks from 20 to 30 min of retention time (Fig.
0%. The recovery experiment was carried out to evaluate the accuracy of the method. Known amounts of lobetyolin was added to the sample and extracted accordingly; the extracted material was subjected for analysis, and the content of the marker was calibrated.

대상 데이터

modesta and Codonopsis tangshen derived from different regions of China. Eighteen different populations of Codonopsis roots from various geographical regions were collected; each chosen population contained over 10 different batches of samples. Table 1 shows there are a great variation regarding the content of lobetyolin from different regions of China, even if they are from the same region of cultivation.
Dong according to morphological characteristics. The voucher specimens were deposited in Hong Kong University of Science and Technology. The dried samples were kept in silica gel.
aEighteen populations of Codonopsis roots were collected as described in the experimental section, ^bFrom 10 to 15 individual samples from each population were analyzed. All roots were collected in Fall of 2003; they were ~2-3 years of growth.

데이터처리

A matrix of 18×15 was obtained, which gave the content differences of 15 constituents among the tested 18 populations, as listed in Table 1. In the hierarchical clustering analysis, a method called average linkage between groups was applied, and Pearson correlation was selected as measurement, and the result was shown in Fig. 4. The tested 18 populations of Codonapsis roots were divided into two main clusters: samples 1-5 from Codonopsis pilosula as duster one; samples 6-14 from Codemopsis pilosula var.

참고문헌 (17)

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Regions in China identification and quality control of radix Codonopsis by chemical fingerprint: Evaluation of lobetyolin from different cultivated 원문보기

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Regions in China identification and quality control of radix Codonopsis by chemical fingerprint: Evaluation of lobetyolin from different cultivated 원문보기

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