[논문]자궁내막증 환자의 정상위치 및 이소성 자궁내막에서의 Osteopontin의 발현

구윤희; 김정훈; 김지선; 이영진; 김성훈; 채희동; 강병문

자궁내막증 환자의 정상위치 및 이소성 자궁내막에서의 Osteopontin의 발현
Expression of Osteopontin in Eutopic and Ectopic Endometrial Tissues in Endometriosis 원문보기

대한생식의학회지= The Korean journal of reproductive medicine, v.34 no.3, 2007년, pp.149 - 157

구윤희 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 김정훈 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 김지선 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 이영진 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 김성훈 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 채희동 (울산대학교 의과대학 서울아산병원 산부인과학교실) , 강병문 (울산대학교 의과대학 서울아산병원 산부인과학교실)

초록
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목 적: 본 연구는 세포간 부착과 유착에 관여하는 당단백질의 하나인 osteopontin (OPN)의 mRNA와 단백질의 발현 양상을 자궁내막증 환자의 정상위치 자궁내막조직과 이소성 자궁내막조직, 대조군의 자궁내막조직에서 비교, 분석하기 위해 시행되었다. 연구방법: 개복 또는 복강경 수술을 통하여 자궁내막증으로 확진된 환자 32명을 연구 대상으로 하였고 같은 시기에 자궁경부 상피내암 또는 자궁내막증 이외의 양성 부인과 질환으로 수술적 치료를 받은 환자 34명을 대조군으로 하였다. 수술 시 자궁내막종 또는 복강내 자궁내막 이식물로부터 이소성 자궁내막조직을 얻었고, 동시에 정상위치 자궁내막조직을 생검하였다. 자궁내막조직 내의 OPN mRNA의 발현 정도는 실시간 역전사 중합효소연쇄반응을 이용하여 비교하고 단백질 발현에는 western blot 분석을 사용하였다. 각 군 간의 비교에는 ANOVA와 Krusxal-Wallis test를 사용하였으며 p-value 0.05 미만을 유의한 것으로 판정하였다. 결 과: 월경주기의 증식기와 분비기 모두에서 자궁내막중 환자의 정상위치 및 이소성 자궁내막조직에서의 OPN mRNA의 발현은 대조군의 자궁내막조직에 비해 유의하게 높은 것으로 나타났다 자궁내막중 환자에서 정상위치 자궁내막조직의 경우 OPN mRNA의 발현은 증식기에 비해 분비 기에서 의미 있게 증가하였으나 이소성 자궁내막 조직에서는 분비기에서 뚜렷하게 감소하는 양상으로 나타났다. OPN 단백질의 발현도 mRNA의 발현과 마찬가지로 자궁내막중 환자에서 유의하게 높은 것으로 확인되었다. 결 론: 본 연구의 결과는 자궁내막증이 있는 여성의 정상위치 및 이소성 자궁내막 조직에서의 증가된 OPN mRNA 및 단백질의 발현이 자궁내막 조직의 유착 및 침습에 관여할 수 있음을 시사한다.

Abstract ▼ AI-Helper

Objective: This study was performed to compare the expression of osteopontin (OPN) mRNA and protein in the eutopic and ectopic endometrial tissues in women with endometriosis and endometrial tissues in women without endometriosis. Methods: A total of 32 women with histologically confirmed endometriosis were recruited for study group. For controls, 34 women undergoing operative treatment for cervical intraepithelial neoplasia (CIN) or benign gynecologic condition other than endometriosis were recruited. At the time of laparoscopy or laparotomy, a biopsy specimen was taken from the endometrial cavity and peritoneal endometrial implant or endometrioma whenever appropriate. We employed real time quantitative RT-PCR to quantify OPN mRNA expression of these tissues and performed western blot analysis to measure the quantity of OPN. Results: The expression of OPN mRNA was significantly higher in both eutopic and ectopic endometrial tissues of women with endometriosis than in endometrial tissues of controls during both proliferative and secretory phase. In the eutopic endometrial tissue of women with endometriosis, OPN mRNA expression significantly increased during the secretory phase compared to the proliferative phase in women with endometriosis as well as controls. However, in the ectopic endometrial tissue, OPN mRNA expression significantly decreased during the secretory phase compared to the proliferative phase. The expression of OPN protein was significantly higher in women with endometriosis than in controls. Conclusion: This study shows the marked expression of OPN mRNA and protein in eutopic and ectopic endometrial tissues in women with endometriosis may be associated with the adhesion and invasion of endometrial explants.

주제어

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제안 방법

Quantitative real-time PCR was performed in the ABI PRISM 7700 Sequence Detection System (Applied biosystems, Foster city, CA) by using SYBR Green I kit. In a total volume of 20 μ 1, each reaction contained 2 |il of chromosomal DNA (cDNA), 10X Master Mix (consisting of SYBR Green I stain, MgCk, dNTPs, and Tag DNA polymerase), each primer, with standard or nuclease-free water as negative control.

대상 데이터

The study group was recruited from women undergoing surgery for endometriosis beginning in October 2004 in Asan Medical Center (Seoul, republic of Korea). All tissue samples were obtained with foil and informed consent.
This study group included 32 women aged 20~47 years. All patients did not receive hormonal treatments, such as GnRH agonist or sex steroids, and did not use intrauterine contraception for >6 months before surgery.

데이터처리

Statistical analysis was performed using ANOVA, Kruskal-Wallis test. The statistical significance was defined as aP value of <.

이론/모형

All patients did not receive hormonal treatments, such as GnRH agonist or sex steroids, and did not use intrauterine contraception for >6 months before surgery. Endometriosis was confirmed by pathological examination of biopsied tissue, and the extent of disease was staged according to the revised American Society of Reproductive Medicine (ASRM) classification of endometriosis.

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