[논문]Further Evidence for the Role of Cantharidin in the Mating Behaviour of Blister Beetles (Coleoptera: Meloidae)

Nikbakhtzadeh, Mahmood Reza; Hemp, Claudia; Ebrahimi, Babak

doi:10.1080/17386357.2007.9647327

Further Evidence for the Role of Cantharidin in the Mating Behaviour of Blister Beetles (Coleoptera: Meloidae) 원문보기

Integrative biosciences, v.11 no.2, 2007년, pp.141 - 146

Nikbakhtzadeh, Mahmood Reza (Department of Medical Parasitology & Entomology, Tarbiat Modares University) , Hemp, Claudia (Department of Animal Ecology II, University of Bayreuth) , Ebrahimi, Babak (Department of Entomology, The Ohio State University)

Abstract ▼ AI-Helper

Cantharidin is produced by blister beetles (Coleoptera: Meloidae) and smaller oedemerid beetles (Coleopetra: Oedemeridae) and is found in hemolymph and various tissues. The function of cantharidin in the courtship behaviour of meloids had never been fully established. Our studies show a correlation between density of cuticular pores and cantharidin titre of the scape and pedicel segments of male specimens of the East African species of Epicauta nyassensis (Haag-Rutenberg, 1880) (Coleoptera: Meloidae). Light microscopy of semi-thin cross sections of the male scape and pedicel indicates that there are many canal shaped structures that stretch from the antennal hemolymph to the antennomere surface. These structures may be tubules, which transport cantharidin circulating in the hemolymph to the surface, where the compound can be released via cuticular pore openings. Analyses of the head capsule and antennal segments of E. nyassensis females which had been copulated with males revealed low titre of cantharidin in the first two antennal segments. The density of the scape and pedicel pores of females was to some extent higher than the density of these pores on flagellum; however it was considerably lower than that of the males. Interestingly, no tubular cell or other transport structures were found in the cross sectioning of the female antennomeres or on the integument surface. During mating, male antennomeres, as well as cantharidin containing pores which are located on the $1^{st}\;and\;2^{nd}$ antennomeres, come into direct contact with the female antennae and may release cantharidin to their surface. Female E. nyassensis may be able to discriminate the opposite sex with abundant reserves of cantharidin prior to mating. This is another evidence that cantharidin function in close range sexual selection.

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대상 데이터

5 KV and AC: 40 mA. Ten random areas of the gold coated samples were examined under a Cambridge electron microscope (Cambridge Instruments®) and photograhps were taken with a Nikon Coolpix 995 digital camera. To determine pore density within any frame of a sample, cuticular pores were counted over an area of 24.
They were collected manually while sitting on different shrubs of the families Astraceae, Compositeae and Leguminoseae. The insects were transported to the Medical Entomology Department at Tarbiat Modares University, where they were maintained in the laboratory.

데이터처리

9% confidence level. Cuticular pore density in experiments with more than two groups was analysed by a one-way ANOVA as a parametric test, and significant means were separated with Tukey HSD posthoc test at the P < 0.01 and P< 0.001 levels. Whenever cuticular pore density was compared within two groups, student /-test was used to indicate the significance atP = 0.
A: Median (± minimum and maximum data, n = 5) titre of cantharidin (ng/mg dry weight) in the male antennal segments of Epicauta nyassensis. Kruskal-Wallis ANOVA test, P< 0.05. B: Mean number (±SD, n = 5) of cuticular pores on the male antennal segments of Epicauta nyassensis per 24.
A: Median (± minimum and maximum data, n = 5) titre of cantharidin (ng/mg dry weight) in the male antennal segments of Epicauta nyassensis. Kruskal-Wallis ANOVA test, P< 0.05. B: Mean number (±SD, n = 5) of cuticular pores on the male antennal segments of Epicauta nyassensis per 24.
3. Mean number (±SD, n = 5) of cuticular pores on the scape (A) and pedicel (B) segments of male and female antennae per 24.5 (um2 of SEM visible field, Epicauta nyassensis ***: statistically significant difference using Mann-Whitney U-test, at P< 0.001 level of confidence.
5 um² of SEM visile field. Rectangles with different letter on top indicate statistically significant differences from each other using ANOVA, Tukey HSD-test at P< 0.001 level of confidence.
5 pm2 of SEM visible field. Rectangles with the same letter on top are not statistically different from each other using ANOVA, Tukey HSD-test at P< 0.05 level of confidence.
The data of the internal distribution of cantharidin were analysed by Kruskal-Wallis ANOVA test. If the test showed any significant difference within an experiment, the statistically significant group(s) was determined by a Tukey Kramer test.
001 levels. Whenever cuticular pore density was compared within two groups, student /-test was used to indicate the significance atP = 0.001 level, unless variable P was more than 1 and therefore analysed by Mann- Whitney t7-test. Apart from the Tukey-Kramer test, the statistical analyses were carried out using Statistica Package (Kernel version 5.

이론/모형

001 level, unless variable P was more than 1 and therefore analysed by Mann- Whitney t7-test. Apart from the Tukey-Kramer test, the statistical analyses were carried out using Statistica Package (Kernel version 5.5 A, StatSoft Inc., 1999, USA).

성능/효과

After two weeks of laboratory maintenance, five males and five virgin females out of 30 were selected and placed in a screened cage (40 x 40 x 40 cm) until the sexual activity was observed. Subsequently, they were sacrificed and dissected two days following copulation.
If the test showed any significant difference within an experiment, the statistically significant group(s) was determined by a Tukey Kramer test. Repeatability of chemical experiments was tested over 5 samples (95% confidence level), and for determination of the cuticular pore density, 10 random locations of each of 5 samples were inspected at 99.9% confidence level. Cuticular pore density in experiments with more than two groups was analysed by a one-way ANOVA as a parametric test, and significant means were separated with Tukey HSD posthoc test at the P < 0.

후속연구

We hereby provide further evidence linking the probable role of cantharidin in close range sexual selection in family Meloidae. Further studies are needed to confirm the role of cantharidin as a precopulatory discriminator.

참고문헌 (24)

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