[국내논문]음나무(Kalopanax pictus) 추출물과 비타민 C의 항산화, 항암 및 면역활성 상승효과 Synergistic Effect of Methanol Extract from Kalopanax pictus and Ascorbic Acid on Antioxidant, Anticancer and Immunomodulatory Activities원문보기
음나무 (Kalopanax pictus, 이하 KP)의 추출물과 ascorbic acid(AA)의 DPPH와 ABTS 라디칼, FRAP 및 NO 소거능 상승효과를 조사하였다. 라디칼 소거능과 항산화능은 농도에 비례하여 증가하였으며, AA 첨가에 의해서 그 활성이 향상되었다. 인간 간암세포주에 대한 KP추출물+AA의 항암능은 MTT법에서 우수한 효과를 나타내었으며 세포 사멸을 유도하였다. 또한 KP 추출물+AA는 세포주기의 G0/G1-phase 또는 G2/M-phase에 영향을 미쳤으며, 농도 의존적인 효과를 나타내었다. 그리고 KP추출물+AA는 대식세포주를 이용한 NO생성과 억제의 면역활성 영향을 나타내었다. 결론적으로 KP추출물의 항산화, 항암 및 면역조절 효과는 KP추출물 단독으로 처리할 때보다, KP 추출물과 AA를 동시에 처리한 경우가 더욱 효과적이었다.
음나무 (Kalopanax pictus, 이하 KP)의 추출물과 ascorbic acid(AA)의 DPPH와 ABTS 라디칼, FRAP 및 NO 소거능 상승효과를 조사하였다. 라디칼 소거능과 항산화능은 농도에 비례하여 증가하였으며, AA 첨가에 의해서 그 활성이 향상되었다. 인간 간암세포주에 대한 KP추출물+AA의 항암능은 MTT법에서 우수한 효과를 나타내었으며 세포 사멸을 유도하였다. 또한 KP 추출물+AA는 세포주기의 G0/G1-phase 또는 G2/M-phase에 영향을 미쳤으며, 농도 의존적인 효과를 나타내었다. 그리고 KP추출물+AA는 대식세포주를 이용한 NO생성과 억제의 면역활성 영향을 나타내었다. 결론적으로 KP추출물의 항산화, 항암 및 면역조절 효과는 KP추출물 단독으로 처리할 때보다, KP 추출물과 AA를 동시에 처리한 경우가 더욱 효과적이었다.
The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), nitric oxide (NO) scavenging activities and ferric- reducing/antioxidant power (FRAP) assay against extracts of Kalopanax pictus (KP) were measured. Radical scavenging and antioxidant activities we...
The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), nitric oxide (NO) scavenging activities and ferric- reducing/antioxidant power (FRAP) assay against extracts of Kalopanax pictus (KP) were measured. Radical scavenging and antioxidant activities were increased depend on the concentration and the effects were enhanced by ascorbic acid (AA). KP extracts and AA had a good anti-proliferating activity against HepG2 cells by MTT assay and induces cells apoptosis, which was demonstrated by flow cytometric analysis. KP extracts and AA caused the arrest of cell-cycle progression at either G0-G1-phase or G2/M-phase, which might be depending upon the KP extracts concentration. In addition, KP extracts and AA are effective in enhancing immunity and nitric oxide production by RAW 264.7 macrophages cells. KP extracts and AA inhibited tumor cell growth and exerted antioxidant effects as compared to controls. These results demonstrate that simultaneous AA and KP extracts treatment could be useful in preventing the oxidative damage and anti-proliferating HepG2 cells, and are effective in enhancing immunomodulatory and antioxidant activity.
The 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), nitric oxide (NO) scavenging activities and ferric- reducing/antioxidant power (FRAP) assay against extracts of Kalopanax pictus (KP) were measured. Radical scavenging and antioxidant activities were increased depend on the concentration and the effects were enhanced by ascorbic acid (AA). KP extracts and AA had a good anti-proliferating activity against HepG2 cells by MTT assay and induces cells apoptosis, which was demonstrated by flow cytometric analysis. KP extracts and AA caused the arrest of cell-cycle progression at either G0-G1-phase or G2/M-phase, which might be depending upon the KP extracts concentration. In addition, KP extracts and AA are effective in enhancing immunity and nitric oxide production by RAW 264.7 macrophages cells. KP extracts and AA inhibited tumor cell growth and exerted antioxidant effects as compared to controls. These results demonstrate that simultaneous AA and KP extracts treatment could be useful in preventing the oxidative damage and anti-proliferating HepG2 cells, and are effective in enhancing immunomodulatory and antioxidant activity.
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대상 데이터
TNF-az IL-6 and GM-CSF (Pharminggn, San Diego, USA) levels in macrophage culture medium were determined by enzyme-linked immunosorbent assay. DMEM (Dulbeco's modified Eagle's medium), FBS (fetal bovine serum), 0.05% trypsin-0.02% EDTA and 100 units/ml penicillin-streptomycin were purchased from GIBCO Co. (Grand Island, NY, USA). RAW264.
(Grand Island, NY, USA). RAW264.7 cells and HepG2 cancer cells were purchased from the Korean Cell Line Bank (Seoul, Korea).
데이터처리
Analysis of variance (ANOVA) was used for all data analysis, followed by Dunnett's test for multiple comparisons. Statistical significance was defined as p<0.
이론/모형
7 cells (5xl05 cells/well) were stimulated with LPS or KP and KP+AA for the production of nitric oxide. After 24 hours of culture, the amounts of NO production were measured by the Griess method as described under Materials and Methods. 1The values given are the mean±SD of at least three independent experiments, each performed in triplicate.
성능/효과
KP and KP+AA quenched RNS effectively. Therefore, in this study, NO scavenging activity correlate with total phenolics content, this suggested that KP and KP+AA have the critical ability for the NO scavenging activity. A correlation was found for the total phenolic content and the FRAP assays and this is in correspondence with Schlesier et al.
3, cells treated with the KP and KP+AA for 24 hr slightly decreased in the number of cells in S phase whereas accumulated in the G2/M and Ml phase of the cell cycle. The results indicated that the KP and KP+AA could suppress HepG2 liver cancer cell lines proliferation via the cell cycle blockage.
to tumorigenesis [6]. Our results demonstrated that the antitumor effects of the KP and KP+AA were similar to that of TNF-az one of the most potent antitumor molecules known [2디. In present study, KP and KP+AA was found to inhibit tumor cell growth and also showed potential as an anticancer agent.
NO has been identified as a major effecter molecule produced by macrophages and is involved in the regulation of apoptosis and in host defenses against microorganisms and tumor cells [3]. In conclusion, KP and KP+AA exhibited a good antioxidant activity in four models studied and showed a good immuno-modulating effect. In addition, our results suggested that cell cycle arrest at G2/M phase and induce apoptosis as a mechanism by which KP and KP+AA an antiproliferative effect.
In conclusion, KP and KP+AA exhibited a good antioxidant activity in four models studied and showed a good immuno-modulating effect. In addition, our results suggested that cell cycle arrest at G2/M phase and induce apoptosis as a mechanism by which KP and KP+AA an antiproliferative effect. However, further investigation at molecular level is required to identify the active components that could induce growth inhibition and establish the possible correlation among the mentioned activities of the KP and KP+AA.
후속연구
As an antioxidant it may be important to support the antiproliferative effect of KP and KP+AA is correlated with its scavenging ROS. Further investigation are needed to determine whether KP and KP+AA induced apoptosis in HepG2 liver cancer cell lines via modulate the intracellular redox status. As for inhibitory effect of KP and KP+AA on cell proliferation were found to be active against the cancer cell lines and showed a concentration-dependent affectivity.
In addition, our results suggested that cell cycle arrest at G2/M phase and induce apoptosis as a mechanism by which KP and KP+AA an antiproliferative effect. However, further investigation at molecular level is required to identify the active components that could induce growth inhibition and establish the possible correlation among the mentioned activities of the KP and KP+AA. Also, more detailed work is required on the protective effects against cancer cell and immuno-modulating effects to know their exact mechanism of action.
However, further investigation at molecular level is required to identify the active components that could induce growth inhibition and establish the possible correlation among the mentioned activities of the KP and KP+AA. Also, more detailed work is required on the protective effects against cancer cell and immuno-modulating effects to know their exact mechanism of action.
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