[논문]Evaluation of White-rot Fungi for Biopulping of Wood

Kang, Kyu-Young; Sung, Jung-Suk; Kim, Dae-Young

doi:10.4489/myco.2007.35.4.205

Evaluation of White-rot Fungi for Biopulping of Wood 원문보기

Mycobiology, v.35 no.4, 2007년, pp.205 - 209

Kang, Kyu-Young (Department of Forest Resources, Dongguk University) , Sung, Jung-Suk (Department of Life Science, Dongguk University) , Kim, Dae-Young (Department of Forest Resources, Dongguk University)

Abstract ▼ AI-Helper

Ergosterol involves in fungal cell growth as a major component in fungal cell membranes. It can be an indicator that shows the fungal activity, and its content depends on the fungal strains, culture, growth conditions and so on. In this study, fungal activities and growth patterns of three white-rot fungi strains isolated in Korea were evaluated by determination of ergosterol contents during the incubation. Wood decay test and chemical analyses of wood were also performed to verify the relationship between fungal activity and wood degrading capacity of white-rot fungi for 60 days. In the results of experiments, it is considered that the test strains selectively degrade large amount of lignin in wood at the early stage of decay. Especially, Phanerochaete chrysosporium showed the best capability on selective degradation of lignin among the test fungi. It is suggested that the determination of ergosterol content in the fungal culture during the incubation is the simple and effective screening method of white-rot fungi for the application to biopulping of wood.

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제안 방법

Chemical composition of wood blocks from the decay test was analyzed to characterize the wood degrad ing pattern of test fungi. It was performed according to the TAPPI Standard Test Methods T 212 om-93 (1% NaOH solubility) and T 222 om-88 (Holocellulose and lignin contents).
In this study, three whit-rot fungi, Phanerochaete chry sosporium, Ceriporiopsis subvermispora, and Trametes versicolor, were tested to evaluate the screening method of white-rot fungi for application to biopulping of wood. These fungi are well known as the wood degrading fungi, and have excellent capacity of lignin degradation in wood.

대상 데이터

KS F 2213. All tests were performed in triplicate. Total test period was 60 days.
Three white-rot fungi strains, P. chrysosporium KCCM 34740, T. versi color KCCM 11258, and C. subvermispora KFRI 21078, were obtained from the Korean Culture Center of Micro organisms (KCCM, Seoul, Korea) and the Korea Forest Research Institute (KFRI, Seoul, Korea). The strains were inoculated and grown in YM broth (Difco 271120) and potato dextrose liquid medium at 29 ± 1, RH 70 ± 1% for 7 days with shaking.
Twenty year old pine (Pinus densiflora S. et Z.) and poplar (Populus alba x glandulosd) were obtained from Dongguk University Research Forest in Namyangju, Korea in January 2001. The logs were debarked and then sawed immediately after felling.

이론/모형

pattern of test fungi. It was performed according to the TAPPI Standard Test Methods T 212 om-93 (1% NaOH solubility) and T 222 om-88 (Holocellulose and lignin contents). The sound wood block was used as the control.
Wood decay test for each strain was performed according to the Korean Standard Test Meth ods KS F 2213. All tests were performed in triplicate.

성능/효과

In the results of ergosterol content measurement and wood decay test, all of three test fungi showed the excel lent ability to degrade the wood by the high fungal activ ity during the early stage of decay. Especially, P.
In the results of the experiments, P. chrysospo rium have excellent colonization and delignification capa bilities on a variety of different substrates, softwood and hardwood. Therefore, it is considered that P.
In the wood block decay test during total 60 days, all test fongi showed the highest increasing rate of weight loss in the first 10 days of test period (Fig. 2 and 3). According to the progress of decay, the weight loss of wood was increased, but the increasing rate of weight loss showed a gentle slope after 10 days of test period.
In the result of ergosterol contents measurement, all test fungi showed the excellent growth capability in the early stage of incubation. It was verified that P. chrysos-porium and C. subvermispora especially have high fun gal activity and growth capability within 10 days of incubation in the optimum growth condition.
The weight loss of poplar wood block was higher than pine wood block, Therefore, it is considered that all test fungi prefer the hardwood to the softwood. P. chrysosporium showed the highest value of weight loss among the test fungi in the first 10 days of test period. It was 6.

참고문헌 (11)

1 Akhtar M Attridge MC Myers GC Blanchette RA Biomechanical pulping of loblolly pine chips with selected white-rot fungi Holzforschung 1993 47 36 40

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2 Akhtar M Kirk TK Blanchette RA Biopulping: An overview of consortia research Proc. of the 6th ICBPPI 1999 187 192
3 Blanchette RA Burnes TA Eerdmans MM Akhtar M Evaluating isolates of Phanerochaete chrysosporium and Ceriporiopsis subvermispora for use in biological pulping processes Holzforschung 1992 46 109 115

상세보기
4 Ekblad A Wallander H Nasholm T Chitin and ergorsterol combined to measure total and living fungal biomass in ectomycorrhizas New Phytol 1998 138 143 149
5 Hart MR Brokes PC Effects of two ergosterol inhibiting fungicides on soil ergosterol and microbial biomass Soil Biol Biochem 1996 28 885 892

상세보기
6 Koo CD Cho NS Kim JS Park JI Variation of ergosterol content in Lentinula edodes culture J Korean Wood Sci Technol 2000 28 65 70
7 Messner K Koller K Wall MB Akhtar M Scott GM Fungal treatment of wood chips for chemical pulping Environmentally Friendly Technologies for the Pulp and Paper Industry New York John Wiley & Sons, Inc 1998 382 419
8 Pasanen AL Yli-Pietila K Pasanen P Kalliokoski P Tarhanen J Ergosterol content in various fungal species and biocontaminated building materials Appl Environ Microbiol 1999 65 138 142 9872771

상세보기
9 Pennanen T Fritze H Vanhala P Kiikkila O Neuvonen S Baath E Structure of a microbial community in soil after prolonged addition of low levels of simulated acid rain Appl Environ Microbiol 1998 64 2173 2180 9603831

상세보기
10 Seitz LM Sauer DB Burroghs R Moohr HE Hubbard JD Ergosterol as measure of fungal growth Phytopathology 1979 69 1202 1203
11 Weete JD Gandhi SR Biochemistry and molecular biology of fungal sterols Esser K Lemke PA The Mycota. A comprehensive treatise on fungi as experimental systems for basic and applied research Berlin Springer 1996 421 438

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