[논문]생분해성 광활성 물질 5-aminolevulinic acid의 제초활성

천상욱; 김영민

생분해성 광활성 물질 5-aminolevulinic acid의 제초활성
Herbicidal Effect of 5-Aminolevulinic Acid, a Biodegradable Photodynamic Substance 원문보기

농약과학회지 = The Korean journal of pesticide science, v.11 no.1, 2007년, pp.38 - 45

천상욱 (광주과학기술원 창업기술지원센터 (주)캐러스) , 김영민 (동신대학교 생물자원산업화지원센터 (주)동의나라)

초록
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미생물학적으로 생산된 것과 합성된 2종류의 ${\delta}$-aminolevulinic acid(ALA)가 배추의 생장과 엽록소 함량에 미치는 제초활성을 탐색하기 위해 실험실 및 온실시험을 수행하였다. 초기 유묘의 생장에 미치는 ALA의 효과는 농도에 비례하여 반응을 보이며 높은 농도에서 유의적인 억제효과를 보였다. 발생 전 및 발생 후 처리된 ALA는 유의적인 정도의 광활성을 나타냈다. 많은 잎을 가진 엽령의 유묘는 적은 엽령의 유묘보다 ALA에 대해 더 내성을 보였다. 미생물학적으로 생산된 것과 합성된 ALA간의 제초효과에 있어서 유의적인 차이는 인정되지 않았다 그러나 합성된 ALA의 잔류성과 물리화학적 특성은 미생물학적으로 생산된 ALA의 그것 보다 더 안정적이었다. 따라서 잡초발생 전후 처리한 ALA의 제초활성이 인정되었으며 그 화합물은 천연 미생물 제제로 가치 있는 친환경 잡초방제 수단이 될 것으로 사료되었다.

Abstract ▼ AI-Helper

Laboratory and greenhouse experiments were conducted to determine the herbicidal effect of two types of ${\delta}$-aminolevulinic acid (ALA), microbiologically-produced ALA (Bio-ALA) and synthetically produced ALA (Synthetic-ALA), on plant growth and chlorophyll content of Chinese cabbage. ALA effect on early plant growth was greatly concentration dependant, showing significant inhibition at higher concentrations. Both pre- and post-emergence application of ALA exhibited significant degree of photodynamic phytotoxicity. Older plants with many leaves were more tolerant to ALA than younger plants, showing less injury. No significant difference in herbicidal activity of two types of ALA, Bio-ALA and Synthetic-ALA, on plant height and chlorophyll content of Chinese cabbage was observed. However, residual biological activity and physico-chemical properties of Synthetic-ALA were more stable than those of Bio-ALA. Our results suggest that ALA had herbicidal potential with both pre- and post-emergence application, and that the chemical may be a valuable mean of eco-friendly weed control based on natural microbial substance.

주제어

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제안 방법

In addition, biological activity of Bio- and Synthetic-ALAs stored at room temperature was assayed on filter paper treated with 2mM at 5Oday interval for 150 days. Soil samples applied with ALA aqueous solutions of 0, 4, 8, and 12 mM were analyzed at 5~day interval for 20 days.
To determine the persistence of Synthetic-ALA performance when applied into soil surface, residual amount and herbicidal activity of ALA were measured. Growth conditions were the same as those described above.

이론/모형

To determine stability of 16 mM ALA stored at room temperature for 150 days, two types of ALA were analyzed by Lermontova and Grimm method (2000) at 5Oday interval. To 10 μL of supernatant, 0.

성능/효과

At 10 days after ALA application, shoot fresh weight of Chinese cabbage investigated was significantly reduced up to 5 days after application regardless of application concentration. At 0 day after soil application, ALA of 4, 8, and 12 mM reduced shoot growth of Chinese cabbage by 49, 70, and 79%, respectively, compared with control. However, from 5 to 20 days after soil application, ALA applied did not affect shoot growth of Chinese cabbage (Fig.
In conclusion, ALA has been proposed as a tetrapyrroledependent photodynamic herbicide and biodegradable plant growth enhancer, by the action of the protoporphyrinogen IX oxidase (Protox IX). Effect of ALA on early plant growth was greatly concentration dependant, showing significant inhibition at higher concentrations.
The results showed that at 0-days after soil application, more ALA content in the soil was detected with increasing of ALA treatment concentration. However, ALA in the soil was not detected 5 days treatment, indicating that ALA applied in soil could leach into downward or adsorb to organic matters.
ALA at 2 mM reduced shoot fresh weights of Chinese cabbage with 2-, 3~, 4-, and 5~leaf stage by 57~62, 51~52, 4041, and 13-25%, respectively. The results showed that older plants were less affected by ALA than younger plants, showing less injury. However, no significant difference in herbicidal activity between two types of ALA on shoot fresh weight of Giinese cabbage was observed (Fig.

후속연구

5). However, further investigations also needed to elucidate the fate of the ALA in soil.
and post-emergence applications. The fundamental study would be useful for development of ALA as a new bioherbicide that is biodegradable, environmentally sound, and safe to human, animals and crops.

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