치주인대섬유모세포들은 완전탈구 된 치아의 성공적인 재식을 위한 중요한 요소이다. 따라서, 외상으로 인해 완전탈구된 치아의 보존을 위한 보관액을 선택하는 것이 중요하다. 성장인자들과 호르몬들은 치주인대섬유모세포들의 생존을 위한 치료적 제제로 고려되고 있다. Epidermal growth factor(EGF)는 다른 조직에서 재생과 상처 치유 과정의 중요한 역할인자로 대두되고 있다. 따라서, 완전탈구 된 치아를 위한 치료적 적용을 위해 EGF의 세포 증식에 미치는 영향을 평가하였다. 또한 EGF와 tri-iodothyronine(T3)의 혼합액, EGF와 Heparin-binding epidermal growth factor-like growth factor(HB-EGF)의 혼합액이 세포 증식에 미치는 상승 효과를 평가하였다. 치주인대섬유모세포의 세포증식은 EGF 농도가 증가함에 따라 증가하였고, 10 ng/ml 농도에서 최대 세포증식을 보였다. EGF는 상처치유분석에서 상처 치유촉진과 이동성을 보여주었다. EGF에 T3와 HB-EGF를 첨가한 혼합액에서 배양한 세포는 EGF만 처리한 경우보다 세포 증식이 상승되었다. EGF와 T3 혼합액의 상승효과 기전을 유추하기 위해서 RT-PCR로 EGF 수용기의 발현을 확인하였고, T3가 EGF 수용기 발현을 증가시켰음을 확인하였다. 따라서 EGF와, EGF와 T3, EGF와 HB-EGF의 혼합액은 완전탈구된 치아의 치료에 있어 유용한 선택이 될 것이다.
치주인대섬유모세포들은 완전탈구 된 치아의 성공적인 재식을 위한 중요한 요소이다. 따라서, 외상으로 인해 완전탈구된 치아의 보존을 위한 보관액을 선택하는 것이 중요하다. 성장인자들과 호르몬들은 치주인대섬유모세포들의 생존을 위한 치료적 제제로 고려되고 있다. Epidermal growth factor(EGF)는 다른 조직에서 재생과 상처 치유 과정의 중요한 역할인자로 대두되고 있다. 따라서, 완전탈구 된 치아를 위한 치료적 적용을 위해 EGF의 세포 증식에 미치는 영향을 평가하였다. 또한 EGF와 tri-iodothyronine(T3)의 혼합액, EGF와 Heparin-binding epidermal growth factor-like growth factor(HB-EGF)의 혼합액이 세포 증식에 미치는 상승 효과를 평가하였다. 치주인대섬유모세포의 세포증식은 EGF 농도가 증가함에 따라 증가하였고, 10 ng/ml 농도에서 최대 세포증식을 보였다. EGF는 상처치유분석에서 상처 치유촉진과 이동성을 보여주었다. EGF에 T3와 HB-EGF를 첨가한 혼합액에서 배양한 세포는 EGF만 처리한 경우보다 세포 증식이 상승되었다. EGF와 T3 혼합액의 상승효과 기전을 유추하기 위해서 RT-PCR로 EGF 수용기의 발현을 확인하였고, T3가 EGF 수용기 발현을 증가시켰음을 확인하였다. 따라서 EGF와, EGF와 T3, EGF와 HB-EGF의 혼합액은 완전탈구된 치아의 치료에 있어 유용한 선택이 될 것이다.
Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth. Growth factors and hormones could be considered for the therapeutic appli...
Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth. Growth factors and hormones could be considered for the therapeutic application of the maintenance of viable periodontal ligament fibroblasts (PDLFs). Epidermal growth factor (EGF) has been suggested as an important player for the regeneration and wound healing process on other tissues. Therefore, EGF was evaluated for the therapeutic application on avulsed teeth. In addition, the synergic effect of EGF with tri-iodothyronine (T3) and heparin-binding epidermal growth factor-like growth factor (HB-EGF). The cell proliferation of PDLFs was determined by MTT assay and increased dose-dependently up to 10 ng/ml in the presence of EGF. Maximum cellular growth was shown at the concentration of 10 ng/ml EGF. Also, EGF promoted the wound healing of PDLFs examined by in vitro wound healing assay. Combined effects of EGF with T3 or HB-EGF on the proliferation of PDLFs were also studied. Interestingly, EGF showed the synergic effect on the proliferation of PDLFs with T3 and HB-EGF. To find out the mechanism of the synergic effect of EGF and T3, the effect of T3 on the expression of endogenous EGF receptor was determined by RT-PCR. The result was that T3 enhanced the expression of EGF receptor in PDLFs. It suggested that EGF might be a good choice for a therapeutic application, which can be used as combination with T3 and HB-EGF.
Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth. Growth factors and hormones could be considered for the therapeutic application of the maintenance of viable periodontal ligament fibroblasts (PDLFs). Epidermal growth factor (EGF) has been suggested as an important player for the regeneration and wound healing process on other tissues. Therefore, EGF was evaluated for the therapeutic application on avulsed teeth. In addition, the synergic effect of EGF with tri-iodothyronine (T3) and heparin-binding epidermal growth factor-like growth factor (HB-EGF). The cell proliferation of PDLFs was determined by MTT assay and increased dose-dependently up to 10 ng/ml in the presence of EGF. Maximum cellular growth was shown at the concentration of 10 ng/ml EGF. Also, EGF promoted the wound healing of PDLFs examined by in vitro wound healing assay. Combined effects of EGF with T3 or HB-EGF on the proliferation of PDLFs were also studied. Interestingly, EGF showed the synergic effect on the proliferation of PDLFs with T3 and HB-EGF. To find out the mechanism of the synergic effect of EGF and T3, the effect of T3 on the expression of endogenous EGF receptor was determined by RT-PCR. The result was that T3 enhanced the expression of EGF receptor in PDLFs. It suggested that EGF might be a good choice for a therapeutic application, which can be used as combination with T3 and HB-EGF.
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문제 정의
The purpose of this study was to evaluate the effect of EGF alone and combination with T3 or HBEGF on the proliferation of PDLFs and the effect of EGF on the wound healing and therefore to evaluate EGF for a candidate of therapeutical application of for the preservation and regeneration of PDLFs in a traumatically avulsed tooth.
The purpose of this study was to evaluate the effect of EGF on the proliferation of PDLFs. In the treatment of EGF, there was different cellular proliferation between control and 10 ng/ml ; control and 100 ng/ml on the 3rd day with statistical significance (p<0.
가설 설정
2. The optimal concentration of EGF in cellular growth was found at the concentrations of 10 ng/ml.
The study concluded that T3 enhanced EGF receptor gene expression increasing the number of cell surface EGF receptors on renal proximal tubule cells. Therefore, in this study, using RT-PCR, we tested if the addition of T3 have an effect on mRNA expression of endogenous EGFR in PDLFs. As a result, T3 enhanced the expression of EGFR in PDLFs and it may explain the synergic effect of T3 and EGF.
제안 방법
In this study, the effects of EGF alone and the synergic effect of EGF with T3 or HB-EGF on the proliferation of PDLFs and the effect of EGF on the wound healing were examined to evaluate the potential application of EGF as a storage media or a prereplantation conditioner of avulsed tooth. Cell proliferation of PDLFs was increased dose-dependently in the presence of EGF up to a concentration of 10 ng/ml.
In this study, the effects of combination of EGF with T3 or HB-EGF on PDLFs proliferation were evaluated by MTT assay. The combination of EGF at three different concentrations (0.
This study evaluated the effect of EGF alone and combination with T3 or HB-EGF on the proliferation of PDLFs by MTT assay and the effect of EGF on the wound healing by wound healing assay. To understand the synergic effect of EGF with T3, RT-PCR on EGF receptor was done considering the influence of T3 on EGF receptor expression.
대상 데이터
25% trypsin-EDTA in PBS for subculture. All the experiments were done within 5-10 cellular passages.
데이터처리
1). Absorbance of each concentration was tested by the analysis of Tukey’s multiple comparison tests at 95% confidence level.
성능/효과
The combination of 0.01 nM HB-EGF and EGF at every concentration on the 5th day, showed higher value of MTT assay than that treated with EGF alone (Fig. 5). The proliferation of PDLFs was increased dose-dependently in the combined treatment, except for 0.
The proliferation of PDLFs was increased in the group of combination compared with the single treatment. The combination of 0.1 nM HB-EGF and EGF at every concentration on the 5th day, showed higher value of MTT assay than that treated with EGF alone (Fig. 6). The proliferation of PDLFs was increased dose-dependently in the combined treatment on the 5th day.
In this study, the effects of combination of EGF with T3 or HB-EGF on PDLFs proliferation were evaluated by MTT assay. The combination of EGF at three different concentrations (0.01, 0.1 and 1 ng/ml) with 0.1 nM T3 on the 5th day, showed higher value of MTT assay than that treated with EGF alone (Fig. 3). However, there was no statistical significance.
In case of synergic effect of EGF and T3, it has been reported that T3 modulates EGF-induced renal proximal tubule cell proliferation by an effect on the EGF receptorng/ml20). The study concluded that T3 enhanced EGF receptor gene expression increasing the number of cell surface EGF receptors on renal proximal tubule cells. Therefore, in this study, using RT-PCR, we tested if the addition of T3 have an effect on mRNA expression of endogenous EGFR in PDLFs.
후속연구
From this study we could conclude that EGF could be used as a pretreatment agent for traumatically avulsed teeth. Further study is needed to find out the relationship between these specific growth factors and matrix synthesis by PDLFs. Also, we need to proceed in vivo experiment using EGF and the combination with T3 and HB-EGF.
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