[논문]Kaempferol induced the apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 cells

Choi, Eun-Jeong; Ahn, Woong-Shick

doi:10.4162/nrp.2008.2.4.322

[국내논문] Kaempferol induced the apoptosis via cell cycle arrest in human breast cancer MDA-MB-453 cells 원문보기

Nutrition research and practice, v.2 no.4, 2008년, pp.322 - 325

Choi, Eun-Jeong (Cancer Research Institute, The Catholic University of Korea) , Ahn, Woong-Shick (Cancer Research Institute, The Catholic University of Korea)

Abstract ▼ AI-Helper

The aim of present study was to investigate the effects of kaempferol on cellular proliferation and cell cycle arrest and explore the mechanism for these effects in human breast carcinoma MDA-MB-453 cells. Cells were treated with kaempferol at various concentrations (ranging from 1 to $200\;{\mu}M$) for 24 and 48 hrs. Kaempferol significantly inhibited cancer cell growth in cells exposed to 50 and $10\;{\mu}M$ of kaempferol and incubated for 24 and 48 hrs, respectively. Exposure to kaempferol resulted in cell cycle arrest at the G2/M phase. Of the G2/M-phase related proteins, kaempferol down-regulated CDK1 and cyclin A and B in cells exposed to kaempferol. In addition, small DNA fragments at the sub-G0 phase were increased by up to 23.12 and 31.90% at 10 and $50\;{\mu}M$ incubated for 24 and 48 hrs, respectively. The kaempferol-induced apoptosis was associated with the up-regulation of p53. In addition, the phosphorylation of p53 at the Ser-15 residue was observed with kaempferol. Kaempferol inhibits cell proliferation by disrupting the cell cycle, which is strongly associated with the induction of arrest at G2/M phase and may induce apoptosis via p53 phosphorylation in human breast carcinoma MDA-MB-453 cells.

Keyword

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제안 방법

Therefore, the present study was performed to investigate the effects of kaempferol on cell proliferation and apoptosis and explored the mechanism for these effects in human breast carcinoma MDA-MB-453 cells.
To determine whether the anti-proliferative activity of kaempferol would lead to cell cycle arrest, we analyzed the cell cycle distribution of MDA-MB-453 cells treated with kaei叩fer이 using fluorescence-activated cell sorting (FACS) under the treatment conditions at which kaempferol had a significant result (50 and 10 μM for 24 and 48 hrs, respectively). As 아lovm in Fig.

대상 데이터

Human breast cancer MDA-MB-453 cells were purchased from the KCLB (Korean Cell Line Bank, Korea). Cells were routinely maintained in RPMI 1640 (Invitrogen [Molecular Probes], Gibco, Carlsbad, CA, USA), supplemented with 10% FBS and antibiotics (50 U/ml of penicillin and 50 ug/ml streptomycin, Gibco) at 37 ℃ in a humidified atmosphere containing 5% CO₂.

데이터처리

Data were analyzed by one-way analysis of variance followed by Dunnetfs multiple comparison test (Sigma Stat, Jandel, San Rafael, CA, USA). For all conparisons, differences were considered statistically significant at P<0.

이론/모형

Cell viability was determined using the MTT assay. At 24 and 48 hrs point, the cells exposed to kaempferol were added to methyl thiazolyl tetrazolium (MTT).

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