선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 25% xylene cyanol and 40% sucrose) and separated on 2% agarose gels. After the gel was stained with ethidium bromide, DNA signals on the gel were imaged under ultraviolet light using a Bioprofile image analysis system (Viber Lourmat, Marne la Vallee, France).
대상 데이터
- Acrylamide, bisacrylamide and N,N,N’,N’-tetramethylethylenediamine were purchased from Bio-Rad (Hercules, CA, USA).
- Fibroblast growth factor (FGF)-2, FGF-4, hepatocyte growth factor (HGF) and epithelial growth factor (EGF) were purchased from Peprotech (Princeton, NJ, USA). AuroprobeTMBL PLUS goat anti-mouse immunoglobulin G (IgG) and reagent A and B were purchased from Amersham (Buckinghamshire, England). Collagenase A and DNase were purchased from Roche (Rotkreuz, Switzerland).
- AuroprobeTMBL PLUS goat anti-mouse immunoglobulin G (IgG) and reagent A and B were purchased from Amersham (Buckinghamshire, England). Collagenase A and DNase were purchased from Roche (Rotkreuz, Switzerland). Oligo (d) T and avian myeloblastosis virus reverse transcriptase (AMV-RT) were purchased from Promega (Madison, WI, USA).
- Oligo (d) T and avian myeloblastosis virus reverse transcriptase (AMV-RT) were purchased from Promega (Madison, WI, USA). Deoxyribonucleotides (dNTPs) and Taq polymerase were purchased from Fermentas (Hanover, MD, USA). RNase inhibitor was purchased from Takara (Japan).
- Acrylamide, bisacrylamide and N,N,N’,N’-tetramethylethylenediamine were purchased from Bio-Rad (Hercules, CA, USA). Mouse monoclonal antibodies against human albumin (ALB) and human alpha fetoprotein (AFP) were purchased from Zymed (San Francisco, CA, USA) and human cytokeratin 18 (CK18) and detection kit was from Dako (Carpinteria, CA, USA). Fibroblast growth factor (FGF)-2, FGF-4, hepatocyte growth factor (HGF) and epithelial growth factor (EGF) were purchased from Peprotech (Princeton, NJ, USA).
- Collagenase A and DNase were purchased from Roche (Rotkreuz, Switzerland). Oligo (d) T and avian myeloblastosis virus reverse transcriptase (AMV-RT) were purchased from Promega (Madison, WI, USA). Deoxyribonucleotides (dNTPs) and Taq polymerase were purchased from Fermentas (Hanover, MD, USA).
이론/모형
- Total RNA was isolated using Trizol method. They were allowed to stand at 65℃ for 5 min and was quantitated spectrophotometrically.
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