[논문]한약재인 황금의 궤양성 실험동물에 대한 장간막 임파절 임파구의 면역글로블린 수준, T세포집단, 사이토카인 생성의 조절작용

임병우; 박표잠; 최완수; 김종대

한약재인 황금의 궤양성 실험동물에 대한 장간막 임파절 임파구의 면역글로블린 수준, T세포집단, 사이토카인 생성의 조절작용
Scutellaria baicalensis Modulates Cytokine Production, T Cell Population and Immunoglobulin Level by Mesenteric Lymph Node Lymphocytes in Experimental Mice with Colitis 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.16 no.2, 2008년, pp.100 - 105

임병우 (건국대학교 의료생명대학 생명과학부) , 박표잠 (건국대학교 의료생명대학 생명과학부) , 최완수 (건국대학교 의학전문대학원 면역학 교실) , 김종대 (강원대학교 BT특성화 대학)

Abstract ▼ AI-Helper

We previously examined extracts, isolated from Scutellaria baicalensis (SB), chemical mediators, and IgE by mesenteric lymph node (MLN) lymphocytes in rats. The present study was to evaluate the effects of extracts of SB on the MLN lymphocytes function of mice given orally by 20 mg/kg for 2 weeks with dextran sulfate sodium (DS)-induced colitis. Results show that IgE levels in MLN lymphocytes was low, while IgA was high, in mice given SB compared to that fed water. Concentrations of $Inteferon-{\gamma}$ and interleukin (IL)-2 of T cells by concanavalin A treatment was significantly higher in the SB fed group than the normal group. Activation-induced IL-4 and IL-10 secretion was lower in SB fed mice compared control mice after DS-induced colitis. These results suggested that SB suppresses the inflammation in DS-induced colitis through the modulation of Th1/Th2 balance to down-regulate $Th_2$ response in MLN lymphocytes.

주제어

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문제 정의

On the other hand, very few studies have focused on the effects of SB on chemotherapeutic-induced colitis. The purpose of this current study was to evaluate the intestinal immunoregulatory effects of SB on DS induced colitis in mice. Oral administration of DS in mice induces colitis resembling human UC.

대상 데이터

Four-week old female Balb/c mice were obtained from the Samtako Bio Korea (Osan, Korea). All animal-care techniques were performed within the guidelines approved by the Institutional Animal Care and Use Committee.

데이터처리

The data are presented as mean ± SD. Diflferences between the means of the individual groups were assessed by oneway ANOVA with Duncan's multiple range tests (1955).

이론/모형

Measurements of IgE and IgA were executed using sandwich ELISA methods, as reported previously by Lim et al. (2000).

성능/효과

Our study was to evaluate the effects on extracts of Scutellaria baicalensis (SB) on the MLN lymphocytes function of mice given orally at 20 mg/kg fbr 2 weeks with dextran sulfate sodium (DS)-induced colitis. Results show that IgE levels in MLN lymphocytes was low, while IgA was high, in mice given SB compared to that fed water. Concentrations of inteferon-y and interleukin (IL)-2 by concanavalin A treatment was significantly higher in the SB fed gro니p than the normal group.

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